Pseudogymnoascus sp. VKM F-4520 (FW-2644)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus; unclassified Pseudogymnoascus

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11550 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A094HH73|A0A094HH73_9PEZI Ubiquitin carboxyl-terminal hydrolase OS=Pseudogymnoascus sp. VKM F-4520 (FW-2644) OX=1420915 GN=V502_06173 PE=3 SV=1
MM1 pKa = 7.05VAIKK5 pKa = 10.14PVFALSLLAGLITPALSAAVKK26 pKa = 10.17INAFGDD32 pKa = 4.24SITGSPGCWRR42 pKa = 11.84ALLYY46 pKa = 10.54QKK48 pKa = 10.54LVEE51 pKa = 5.28AGTTDD56 pKa = 2.88IDD58 pKa = 4.5FVGTLPGQGCGIEE71 pKa = 3.98YY72 pKa = 10.6DD73 pKa = 4.23GEE75 pKa = 4.34NDD77 pKa = 3.27GHH79 pKa = 7.39GGFLATGIVADD90 pKa = 3.97NQLPGWLAISQPDD103 pKa = 3.44IVMMQLATNDD113 pKa = 2.73IWSNIATATILDD125 pKa = 4.09AFSALVDD132 pKa = 3.29QMRR135 pKa = 11.84DD136 pKa = 3.28SKK138 pKa = 10.74STMHH142 pKa = 6.54IVVAQITPMDD152 pKa = 4.24PTDD155 pKa = 3.45GCATCEE161 pKa = 3.88AGIIALNDD169 pKa = 5.02AIPAWVAEE177 pKa = 4.17KK178 pKa = 9.96STIEE182 pKa = 4.04SPITVVDD189 pKa = 4.99CYY191 pKa = 10.57TGYY194 pKa = 8.59DD195 pKa = 3.85TATDD199 pKa = 3.96TYY201 pKa = 11.53DD202 pKa = 3.5GVHH205 pKa = 6.79PNDD208 pKa = 3.71SGNVKK213 pKa = 10.25LADD216 pKa = 3.21AWFEE220 pKa = 3.91PLQAAILAASSGTNSTIAA238 pKa = 3.51

Molecular weight: 24.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A094HZ68|A0A094HZ68_9PEZI CUE domain-containing protein OS=Pseudogymnoascus sp. VKM F-4520 (FW-2644) OX=1420915 GN=V502_03319 PE=4 SV=1
AA1 pKa = 7.98DD2 pKa = 3.92SEE4 pKa = 4.62NLVLAADD11 pKa = 4.0PADD14 pKa = 3.86RR15 pKa = 11.84ATSRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84KK22 pKa = 10.04LILLACWVPSSPDD35 pKa = 3.32DD36 pKa = 4.04GEE38 pKa = 4.41AAGSRR43 pKa = 11.84SRR45 pKa = 11.84AARR48 pKa = 11.84MVSWGVVMGDD58 pKa = 3.29STADD62 pKa = 3.32TDD64 pKa = 3.66AGSAKK69 pKa = 10.48GPTEE73 pKa = 3.38KK74 pKa = 10.06RR75 pKa = 11.84RR76 pKa = 11.84EE77 pKa = 3.82GRR79 pKa = 11.84RR80 pKa = 11.84RR81 pKa = 11.84GMQGMSKK88 pKa = 10.43GSWARR93 pKa = 11.84RR94 pKa = 11.84TQVIGADD101 pKa = 3.49TSAEE105 pKa = 3.97EE106 pKa = 4.17RR107 pKa = 11.84GVEE110 pKa = 4.17LMQRR114 pKa = 11.84SLGRR118 pKa = 11.84HH119 pKa = 5.12EE120 pKa = 5.72LSTALRR126 pKa = 11.84NPASEE131 pKa = 4.09RR132 pKa = 11.84RR133 pKa = 11.84LPASATRR140 pKa = 11.84VQYY143 pKa = 11.48DD144 pKa = 3.56DD145 pKa = 4.61ASSHH149 pKa = 5.99GSADD153 pKa = 3.48LLRR156 pKa = 11.84RR157 pKa = 11.84VADD160 pKa = 3.6VAGGSAGGVAGGRR173 pKa = 11.84RR174 pKa = 11.84AANGRR179 pKa = 11.84LWGGGTGGGGSYY191 pKa = 10.6CGG193 pKa = 3.64

Molecular weight: 20.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11550

0

11550

5669913

10

5591

490.9

54.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.923 ± 0.023

1.219 ± 0.007

5.642 ± 0.015

6.258 ± 0.023

3.657 ± 0.013

7.255 ± 0.023

2.272 ± 0.009

5.138 ± 0.015

4.928 ± 0.018

8.807 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.145 ± 0.008

3.745 ± 0.011

5.834 ± 0.022

3.866 ± 0.017

5.792 ± 0.019

8.036 ± 0.026

6.053 ± 0.017

6.196 ± 0.015

1.452 ± 0.008

2.781 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski