Actinotignum urinale

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Actinotignum

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1431 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N6UXI8|A0A2N6UXI8_9ACTO Dipeptidase OS=Actinotignum urinale OX=190146 GN=CJ184_06410 PE=4 SV=1
MM1 pKa = 7.14ATDD4 pKa = 3.42YY5 pKa = 10.32DD6 pKa = 3.89APRR9 pKa = 11.84KK10 pKa = 9.09QDD12 pKa = 3.04EE13 pKa = 4.4EE14 pKa = 4.41LRR16 pKa = 11.84DD17 pKa = 3.62DD18 pKa = 4.81AFPLVAGRR26 pKa = 11.84HH27 pKa = 5.5SDD29 pKa = 3.65QQSGAVDD36 pKa = 3.61EE37 pKa = 5.41DD38 pKa = 3.84EE39 pKa = 4.96VEE41 pKa = 3.96AAEE44 pKa = 4.66GFEE47 pKa = 5.34LPGADD52 pKa = 4.58LSNLEE57 pKa = 4.4LSVNVIPAQDD67 pKa = 3.96DD68 pKa = 3.87EE69 pKa = 5.11FTCSQCFLVHH79 pKa = 6.5HH80 pKa = 7.02RR81 pKa = 11.84SQLDD85 pKa = 3.3HH86 pKa = 6.86MEE88 pKa = 5.38DD89 pKa = 3.82GLPVCADD96 pKa = 3.58CAGG99 pKa = 3.3

Molecular weight: 10.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N6V0U2|A0A2N6V0U2_9ACTO Uncharacterized protein OS=Actinotignum urinale OX=190146 GN=CJ184_01470 PE=4 SV=1
MM1 pKa = 8.08RR2 pKa = 11.84KK3 pKa = 9.28RR4 pKa = 11.84LLKK7 pKa = 10.56LRR9 pKa = 11.84LRR11 pKa = 11.84KK12 pKa = 9.45QPLRR16 pKa = 11.84KK17 pKa = 9.3KK18 pKa = 9.4RR19 pKa = 11.84PIRR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84VKK26 pKa = 10.35LKK28 pKa = 10.48QLLAVSTRR36 pKa = 11.84GKK38 pKa = 10.18SIRR41 pKa = 11.84LSRR44 pKa = 11.84MLMKK48 pKa = 10.81LKK50 pKa = 10.25LKK52 pKa = 10.3PMWHH56 pKa = 6.05VKK58 pKa = 9.16RR59 pKa = 4.4

Molecular weight: 7.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1431

0

1431

498572

30

3001

348.4

38.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.062 ± 0.072

0.842 ± 0.021

5.594 ± 0.057

6.419 ± 0.066

3.43 ± 0.043

8.091 ± 0.063

2.201 ± 0.029

5.973 ± 0.054

4.471 ± 0.063

9.038 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.391 ± 0.026

3.499 ± 0.042

4.673 ± 0.038

3.303 ± 0.036

5.912 ± 0.057

6.115 ± 0.053

6.218 ± 0.047

7.875 ± 0.049

1.302 ± 0.024

2.591 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski