Gremmeniella abietina RNA virus MS2

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; Gammapartitivirus; Gremmeniella abietina RNA virus MS1

Average proteome isoelectric point is 8.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6GYB9|Q6GYB9_9VIRU Putative RNA-dependent RNA polymerase OS=Gremmeniella abietina RNA virus MS2 OX=279540 PE=4 SV=1
MM1 pKa = 7.97SDD3 pKa = 3.28RR4 pKa = 11.84QSEE7 pKa = 4.39ISSSVAPGDD16 pKa = 4.09SVSSRR21 pKa = 11.84GGKK24 pKa = 9.03KK25 pKa = 10.03GKK27 pKa = 9.08PGKK30 pKa = 10.24AEE32 pKa = 3.6RR33 pKa = 11.84AARR36 pKa = 11.84RR37 pKa = 11.84AGQGSTPGVAASSAKK52 pKa = 10.49ASLFSSAGQADD63 pKa = 4.05INPTPGKK70 pKa = 9.98FPVVFATGAGEE81 pKa = 3.97PTRR84 pKa = 11.84DD85 pKa = 3.33AEE87 pKa = 4.15FSYY90 pKa = 11.18DD91 pKa = 3.32FSAVKK96 pKa = 10.17RR97 pKa = 11.84CVVGFGDD104 pKa = 4.52RR105 pKa = 11.84YY106 pKa = 10.25VKK108 pKa = 10.19NARR111 pKa = 11.84YY112 pKa = 9.93SEE114 pKa = 4.46FKK116 pKa = 10.84ANSGVADD123 pKa = 3.91SAFKK127 pKa = 10.68CYY129 pKa = 10.0LAASCLLGLAQQTVHH144 pKa = 6.1AHH146 pKa = 4.87VNMGLPQGDD155 pKa = 4.21FAPVSSSEE163 pKa = 4.01VLNFTAVKK171 pKa = 10.26AYY173 pKa = 9.48IGQFGEE179 pKa = 4.31FAVPSTGTRR188 pKa = 11.84YY189 pKa = 8.97MLADD193 pKa = 3.77YY194 pKa = 10.85QSTVCKK200 pKa = 10.17LVYY203 pKa = 10.39LADD206 pKa = 3.61KK207 pKa = 10.53CLRR210 pKa = 11.84DD211 pKa = 3.77ANHH214 pKa = 5.77NTVLSRR220 pKa = 11.84AWLPMSSGDD229 pKa = 3.22RR230 pKa = 11.84TTKK233 pKa = 10.5VVIAAALSQLLSNAEE248 pKa = 4.15LKK250 pKa = 10.56ISSTVLEE257 pKa = 4.49DD258 pKa = 3.48AVLSGDD264 pKa = 5.79VPDD267 pKa = 3.92AWNAVKK273 pKa = 9.38TVFGDD278 pKa = 3.61APGEE282 pKa = 4.22GVPDD286 pKa = 3.31ARR288 pKa = 11.84DD289 pKa = 3.22RR290 pKa = 11.84FDD292 pKa = 5.59FLFKK296 pKa = 10.7SYY298 pKa = 11.52ADD300 pKa = 3.29VGQFTTAFTTGAASAVLAEE319 pKa = 4.72LHH321 pKa = 6.7LPWSSPSAGHH331 pKa = 7.38LDD333 pKa = 3.04WDD335 pKa = 4.46YY336 pKa = 11.79NPKK339 pKa = 9.1TRR341 pKa = 11.84FSALADD347 pKa = 2.99SWARR351 pKa = 11.84ISASYY356 pKa = 10.96SGFFHH361 pKa = 6.48MASGLSDD368 pKa = 3.5RR369 pKa = 11.84SNALGSEE376 pKa = 4.29SQFVEE381 pKa = 4.12VRR383 pKa = 11.84SIDD386 pKa = 3.46SVTVVKK392 pKa = 9.9TFLALSAPQFSLAACFPPEE411 pKa = 4.4CIFVGSLDD419 pKa = 3.39RR420 pKa = 11.84RR421 pKa = 11.84VVVTTPIPVSQRR433 pKa = 11.84ATEE436 pKa = 4.0FCLRR440 pKa = 11.84DD441 pKa = 3.44WKK443 pKa = 11.18

Molecular weight: 47.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6GYB8|Q6GYB8_9VIRU Coat protein OS=Gremmeniella abietina RNA virus MS2 OX=279540 PE=4 SV=1
MM1 pKa = 7.54SKK3 pKa = 7.84THH5 pKa = 6.11TNLVGVKK12 pKa = 10.16SSPPKK17 pKa = 8.94PKK19 pKa = 10.81GNIRR23 pKa = 11.84QPSASQLRR31 pKa = 11.84FKK33 pKa = 10.66KK34 pKa = 10.46ALARR38 pKa = 11.84ALSVRR43 pKa = 11.84SKK45 pKa = 10.19FLKK48 pKa = 10.18RR49 pKa = 11.84RR50 pKa = 11.84LSKK53 pKa = 11.01PEE55 pKa = 3.66FVQLSAAVKK64 pKa = 9.93KK65 pKa = 10.32QIKK68 pKa = 9.93QEE70 pKa = 4.05TTPVNRR76 pKa = 11.84RR77 pKa = 11.84LNRR80 pKa = 11.84VIDD83 pKa = 3.31IVVRR87 pKa = 11.84LEE89 pKa = 3.65EE90 pKa = 4.29AANSSLSCLDD100 pKa = 3.8RR101 pKa = 11.84PALDD105 pKa = 3.25AAVRR109 pKa = 11.84NLYY112 pKa = 10.43DD113 pKa = 3.28RR114 pKa = 11.84LNLVRR119 pKa = 11.84SNLLEE124 pKa = 3.96AMRR127 pKa = 11.84EE128 pKa = 4.1SSSLSEE134 pKa = 4.42GCSHH138 pKa = 6.67VSKK141 pKa = 10.68KK142 pKa = 10.58SVIHH146 pKa = 6.03LPEE149 pKa = 4.08PVKK152 pKa = 10.62VDD154 pKa = 3.57KK155 pKa = 10.8IFPGFGCISARR166 pKa = 11.84IPSGRR171 pKa = 11.84QVLCCSRR178 pKa = 11.84CSGLRR183 pKa = 11.84NDD185 pKa = 6.09AFFEE189 pKa = 4.3WSGLQPSFIDD199 pKa = 3.8KK200 pKa = 9.76EE201 pKa = 4.27GHH203 pKa = 5.97SMVEE207 pKa = 4.49AYY209 pKa = 8.39PLPKK213 pKa = 9.9SIKK216 pKa = 10.09NISPLDD222 pKa = 3.82FQHH225 pKa = 5.9VWTSVLDD232 pKa = 3.16IHH234 pKa = 7.22YY235 pKa = 10.91SRR237 pKa = 5.53

Molecular weight: 26.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1219

237

539

406.3

45.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.368 ± 2.195

1.723 ± 0.324

5.414 ± 0.559

5.086 ± 0.947

5.168 ± 0.533

7.055 ± 1.155

1.559 ± 0.336

3.035 ± 0.718

5.742 ± 0.82

7.875 ± 0.917

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.133 ± 0.712

3.035 ± 0.415

5.25 ± 0.369

3.363 ± 0.216

7.137 ± 0.901

10.5 ± 1.541

4.594 ± 0.848

7.547 ± 0.731

2.297 ± 0.864

3.117 ± 0.881
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski