Scytalidium lignicola (Hyphomycete)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes;

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12794 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3E2HPT5|A0A3E2HPT5_SCYLI CID domain-containing protein (Fragment) OS=Scytalidium lignicola OX=5539 GN=B7463_g980 PE=4 SV=1
MM1 pKa = 7.33NCDD4 pKa = 3.5DD5 pKa = 4.43TRR7 pKa = 11.84TEE9 pKa = 4.43AIKK12 pKa = 9.83MALVKK17 pKa = 9.92VQNVAYY23 pKa = 10.06RR24 pKa = 11.84AYY26 pKa = 10.75DD27 pKa = 3.51RR28 pKa = 11.84LDD30 pKa = 3.52TLHH33 pKa = 7.45DD34 pKa = 4.47LLNVAQVQLSNFDD47 pKa = 3.67YY48 pKa = 11.37LEE50 pKa = 3.97WSTFNTFEE58 pKa = 4.05TFYY61 pKa = 11.23GQMLVDD67 pKa = 3.89TTRR70 pKa = 11.84TNGGVITEE78 pKa = 4.26TLNYY82 pKa = 9.81LDD84 pKa = 5.37EE85 pKa = 5.43IRR87 pKa = 11.84DD88 pKa = 3.67FLGNIVLAAQNVNADD103 pKa = 3.71QYY105 pKa = 11.57HH106 pKa = 6.5DD107 pKa = 4.13TLSLDD112 pKa = 3.53IWCDD116 pKa = 3.79SAWEE120 pKa = 4.26TTTDD124 pKa = 3.31NNGRR128 pKa = 11.84YY129 pKa = 9.15YY130 pKa = 10.8DD131 pKa = 4.0GPTDD135 pKa = 4.36VMVYY139 pKa = 9.53WDD141 pKa = 3.73GSGNNMGWIDD151 pKa = 4.1PPEE154 pKa = 4.72DD155 pKa = 3.29ATTPADD161 pKa = 6.1LILCTNSPLDD171 pKa = 3.76LGGYY175 pKa = 7.2TINHH179 pKa = 5.98DD180 pKa = 4.03TEE182 pKa = 4.45PVSVITICGDD192 pKa = 3.4ALDD195 pKa = 5.37DD196 pKa = 4.44MISNGMTLTSLGAGDD211 pKa = 4.38IKK213 pKa = 11.14DD214 pKa = 3.78GTDD217 pKa = 3.59LSDD220 pKa = 3.81EE221 pKa = 4.34VEE223 pKa = 4.74KK224 pKa = 10.91IPSFLLLHH232 pKa = 6.23EE233 pKa = 5.24LSHH236 pKa = 6.43AVSVMGRR243 pKa = 11.84GSNTIDD249 pKa = 3.65VKK251 pKa = 10.8FQNPTEE257 pKa = 4.12EE258 pKa = 4.57GVLNAYY264 pKa = 9.78GLEE267 pKa = 4.01NCILLAVNRR276 pKa = 11.84PDD278 pKa = 3.78LTIEE282 pKa = 4.16NADD285 pKa = 3.84TLALLAAALYY295 pKa = 9.05LQQKK299 pKa = 5.91TWWTGEE305 pKa = 4.06AEE307 pKa = 4.16DD308 pKa = 4.04LSYY311 pKa = 10.77RR312 pKa = 11.84ASPSIQFLPPGAAAPP327 pKa = 3.91

Molecular weight: 36.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3E2HHG2|A0A3E2HHG2_SCYLI Uncharacterized protein (Fragment) OS=Scytalidium lignicola OX=5539 GN=B7463_g3444 PE=3 SV=1
MM1 pKa = 7.73ILIRR5 pKa = 11.84IVQPRR10 pKa = 11.84LEE12 pKa = 4.36EE13 pKa = 3.86QDD15 pKa = 3.33QLLMQRR21 pKa = 11.84RR22 pKa = 11.84SEE24 pKa = 3.98RR25 pKa = 11.84DD26 pKa = 2.89RR27 pKa = 11.84KK28 pKa = 10.66NGLRR32 pKa = 11.84QKK34 pKa = 9.86SVRR37 pKa = 11.84YY38 pKa = 9.93DD39 pKa = 2.99IGTVQYY45 pKa = 10.16FSVANLPTTSGQAEE59 pKa = 4.48LTTSEE64 pKa = 4.2TLKK67 pKa = 10.48QLRR70 pKa = 11.84LKK72 pKa = 10.97ALGTGISGQSSDD84 pKa = 3.48YY85 pKa = 10.46TVISRR90 pKa = 11.84AIGHH94 pKa = 6.46RR95 pKa = 11.84PRR97 pKa = 11.84NGLGFGGGDD106 pKa = 4.46DD107 pKa = 5.22DD108 pKa = 6.84DD109 pKa = 5.51NNQTRR114 pKa = 11.84RR115 pKa = 11.84RR116 pKa = 11.84ASGLFCFVGNWGFVCKK132 pKa = 10.31QRR134 pKa = 11.84DD135 pKa = 3.61GMDD138 pKa = 3.6CGLVFLPQSDD148 pKa = 3.79DD149 pKa = 4.47LAWQPAPKK157 pKa = 9.53RR158 pKa = 11.84RR159 pKa = 11.84KK160 pKa = 9.01VVNFPSASLPPQLLSRR176 pKa = 11.84RR177 pKa = 11.84LRR179 pKa = 11.84RR180 pKa = 11.84PWTAAPASCVGG191 pKa = 3.39

Molecular weight: 21.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12794

0

12794

6524524

37

4968

510.0

56.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.981 ± 0.017

1.227 ± 0.008

5.519 ± 0.015

6.25 ± 0.021

3.826 ± 0.012

6.758 ± 0.02

2.283 ± 0.008

5.666 ± 0.014

5.082 ± 0.021

9.029 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.129 ± 0.007

4.048 ± 0.011

5.709 ± 0.02

3.957 ± 0.014

5.613 ± 0.016

8.365 ± 0.023

6.106 ± 0.019

6.037 ± 0.014

1.478 ± 0.008

2.926 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski