Kazachstania naganishii (strain ATCC MYA-139 / BCRC 22969 / CBS 8797 / KCTC 17520 / NBRC 10181 / NCYC 3082 / Yp74L-3) (Yeast) (Saccharomyces naganishii)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Kazachstania; Kazachstania naganishii

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5301 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J7S8N5|J7S8N5_KAZNA Uncharacterized protein OS=Kazachstania naganishii (strain ATCC MYA-139 / BCRC 22969 / CBS 8797 / KCTC 17520 / NBRC 10181 / NCYC 3082 / Yp74L-3) OX=1071383 GN=KNAG0F03290 PE=4 SV=1
MM1 pKa = 6.83NTWKK5 pKa = 10.69DD6 pKa = 3.69SLLSTLWQDD15 pKa = 2.3IDD17 pKa = 3.6AAGVADD23 pKa = 3.74ATVAAGSAAPLQDD36 pKa = 3.16IEE38 pKa = 4.6NICVVVAVWFVVCLLVVLSLSFGACLYY65 pKa = 10.95LSLTGMLCRR74 pKa = 11.84EE75 pKa = 4.22EE76 pKa = 4.05STDD79 pKa = 4.22IEE81 pKa = 4.42KK82 pKa = 10.65QQ83 pKa = 3.22

Molecular weight: 8.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J7RWY6|J7RWY6_KAZNA Arrestin_C domain-containing protein OS=Kazachstania naganishii (strain ATCC MYA-139 / BCRC 22969 / CBS 8797 / KCTC 17520 / NBRC 10181 / NCYC 3082 / Yp74L-3) OX=1071383 GN=KNAG0C04350 PE=4 SV=1
MM1 pKa = 7.69AAQKK5 pKa = 10.46SFKK8 pKa = 10.11IKK10 pKa = 10.14QRR12 pKa = 11.84LAKK15 pKa = 10.05AKK17 pKa = 9.74KK18 pKa = 8.79QNRR21 pKa = 11.84PLPQWVRR28 pKa = 11.84LRR30 pKa = 11.84TNNTVRR36 pKa = 11.84YY37 pKa = 7.58NAKK40 pKa = 9.13RR41 pKa = 11.84RR42 pKa = 11.84NWRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.56MNII51 pKa = 3.1

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5301

0

5301

2648969

21

4928

499.7

56.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.289 ± 0.031

1.286 ± 0.013

5.938 ± 0.023

6.47 ± 0.033

4.245 ± 0.02

5.655 ± 0.035

2.198 ± 0.014

5.647 ± 0.028

6.564 ± 0.031

9.638 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.133 ± 0.011

5.243 ± 0.026

4.581 ± 0.026

4.172 ± 0.026

4.965 ± 0.027

8.314 ± 0.038

6.061 ± 0.027

6.322 ± 0.023

1.061 ± 0.01

3.212 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski