Pseudomonas lundensis

Taxonomy: cellular organisms; Bacteria;

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3977 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D9IJV2|A0A1D9IJV2_9PSED DEAD/DEAH box helicase OS=Pseudomonas lundensis OX=86185 GN=AA042_06585 PE=3 SV=1
MM1 pKa = 7.52TISITYY7 pKa = 9.73NSAFGAFSVQDD18 pKa = 4.14ALSAWSTGFTTAGHH32 pKa = 5.84GTGNTGGFNTGGRR45 pKa = 11.84AMTGEE50 pKa = 4.26QYY52 pKa = 9.45ATHH55 pKa = 6.9GAQGSEE61 pKa = 4.23YY62 pKa = 10.82AFIAASDD69 pKa = 3.65TDD71 pKa = 3.16KK72 pKa = 11.44GLNYY76 pKa = 9.98VFDD79 pKa = 4.81PKK81 pKa = 10.96VSPGDD86 pKa = 3.71NMNHH90 pKa = 5.55YY91 pKa = 10.47LWGEE95 pKa = 4.13LDD97 pKa = 3.73TVTLGTGLQGGNGTDD112 pKa = 4.1FGLNDD117 pKa = 3.36YY118 pKa = 7.45TASFNGLDD126 pKa = 3.92LSAASDD132 pKa = 3.32AGRR135 pKa = 11.84AGNAVQDD142 pKa = 3.96VIYY145 pKa = 10.67GLMKK149 pKa = 10.6GDD151 pKa = 3.52VSGLEE156 pKa = 3.96GALNNLLSGFGLSTDD171 pKa = 3.4STFDD175 pKa = 3.53DD176 pKa = 4.2LAAAGLAHH184 pKa = 6.92TDD186 pKa = 3.18APLAADD192 pKa = 3.42IDD194 pKa = 4.27LVGVQEE200 pKa = 4.75VAQDD204 pKa = 3.19WALAAA209 pKa = 4.59

Molecular weight: 21.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D9IMR0|A0A1D9IMR0_9PSED Aromatic amino acid lyase OS=Pseudomonas lundensis OX=86185 GN=AA042_12175 PE=4 SV=1
MM1 pKa = 8.01DD2 pKa = 6.19LATQPVSTRR11 pKa = 11.84RR12 pKa = 11.84DD13 pKa = 2.99AWCWRR18 pKa = 11.84LIATAISFGLFGLGCLGLRR37 pKa = 11.84LVIFPLLSVLPGTAVRR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84EE56 pKa = 3.7RR57 pKa = 11.84ARR59 pKa = 11.84ATVSRR64 pKa = 11.84LFWLFIRR71 pKa = 11.84SMARR75 pKa = 11.84MGVLTYY81 pKa = 10.45DD82 pKa = 3.33VQGAEE87 pKa = 3.81RR88 pKa = 11.84LGRR91 pKa = 11.84PGQMIIANHH100 pKa = 6.84PSLIDD105 pKa = 3.46VVFLIGLVRR114 pKa = 11.84NANCVVKK121 pKa = 10.63QSLWQNPFTRR131 pKa = 11.84SPVRR135 pKa = 11.84ATQYY139 pKa = 10.3ISNDD143 pKa = 2.98GSVDD147 pKa = 3.45MLDD150 pKa = 3.7NAAQALQEE158 pKa = 4.23GQTLIIFPEE167 pKa = 4.83GTRR170 pKa = 11.84TPPGHH175 pKa = 6.15TPAFHH180 pKa = 7.13RR181 pKa = 11.84GAASIALRR189 pKa = 11.84GAKK192 pKa = 9.81VLTPVIIKK200 pKa = 9.17VSPTTLTKK208 pKa = 10.56AEE210 pKa = 3.82PWYY213 pKa = 9.94RR214 pKa = 11.84IPDD217 pKa = 3.56RR218 pKa = 11.84RR219 pKa = 11.84VHH221 pKa = 5.96FSFRR225 pKa = 11.84VGADD229 pKa = 3.13IDD231 pKa = 3.82PHH233 pKa = 5.63TFAALGPPPKK243 pKa = 10.23ASRR246 pKa = 11.84RR247 pKa = 11.84LNDD250 pKa = 3.3FVHH253 pKa = 7.5DD254 pKa = 3.96YY255 pKa = 10.48FIKK258 pKa = 10.57EE259 pKa = 3.91LATDD263 pKa = 3.68EE264 pKa = 4.45RR265 pKa = 11.84SEE267 pKa = 4.14HH268 pKa = 6.52ANN270 pKa = 3.21

Molecular weight: 30.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3977

0

3977

1276776

29

2135

321.0

35.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.642 ± 0.044

1.003 ± 0.012

5.38 ± 0.028

5.612 ± 0.031

3.647 ± 0.024

7.701 ± 0.039

2.42 ± 0.02

4.894 ± 0.027

3.759 ± 0.031

11.571 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.416 ± 0.019

3.2 ± 0.026

4.875 ± 0.024

4.602 ± 0.031

6.141 ± 0.038

5.803 ± 0.021

5.111 ± 0.03

7.178 ± 0.031

1.428 ± 0.016

2.616 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski