Pseudoroseicyclus aestuarii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Pseudoroseicyclus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3295 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A318SVW2|A0A318SVW2_9RHOB YVTN family beta-propeller protein OS=Pseudoroseicyclus aestuarii OX=1795041 GN=DFP88_102286 PE=4 SV=1
MM1 pKa = 7.07MRR3 pKa = 11.84TSIALLAALGLGSAATAQDD22 pKa = 3.75LSIATGGTGGTYY34 pKa = 9.96YY35 pKa = 10.36PYY37 pKa = 11.05GGALAEE43 pKa = 4.74LIGAHH48 pKa = 5.99VDD50 pKa = 3.39GASAVAEE57 pKa = 4.11VTGASVEE64 pKa = 3.99NMALIAQEE72 pKa = 3.83QSDD75 pKa = 4.06IAFVLADD82 pKa = 3.52TAYY85 pKa = 10.12GAVNGTIDD93 pKa = 4.05PFTDD97 pKa = 3.16RR98 pKa = 11.84AVDD101 pKa = 4.02GQALASIYY109 pKa = 9.94PNAVHH114 pKa = 7.09IVTLAGSGIEE124 pKa = 4.04SLQDD128 pKa = 3.43LVGKK132 pKa = 9.61RR133 pKa = 11.84VSVGAPGSGTEE144 pKa = 4.11VNAEE148 pKa = 3.94QLLSANGITYY158 pKa = 10.73DD159 pKa = 5.48DD160 pKa = 4.17IDD162 pKa = 3.8EE163 pKa = 4.09QRR165 pKa = 11.84LNFNEE170 pKa = 4.05TADD173 pKa = 3.72ALRR176 pKa = 11.84DD177 pKa = 3.49GDD179 pKa = 3.62IDD181 pKa = 3.85AGIWSVGPPTSSILNLAATTDD202 pKa = 3.39IAFVPLTEE210 pKa = 4.44EE211 pKa = 3.93EE212 pKa = 4.17VAAAQEE218 pKa = 4.17VQPVFAAYY226 pKa = 9.15EE227 pKa = 4.09LRR229 pKa = 11.84AGTYY233 pKa = 10.2DD234 pKa = 3.61GVDD237 pKa = 3.14EE238 pKa = 4.75GVATIAIPNVLVVNAAMDD256 pKa = 3.73EE257 pKa = 4.07EE258 pKa = 4.5LAYY261 pKa = 10.7QITSAIFEE269 pKa = 4.56HH270 pKa = 6.63TDD272 pKa = 3.03EE273 pKa = 5.69LIAVHH278 pKa = 6.76PAAEE282 pKa = 4.21DD283 pKa = 3.64TTVDD287 pKa = 3.94FSVNSTPIPFHH298 pKa = 6.81PGAIRR303 pKa = 11.84YY304 pKa = 8.34YY305 pKa = 10.34DD306 pKa = 3.49EE307 pKa = 4.57VGAEE311 pKa = 4.04VSDD314 pKa = 3.78AQRR317 pKa = 11.84PP318 pKa = 3.58

Molecular weight: 32.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A318SQB0|A0A318SQB0_9RHOB Threonine/homoserine/homoserine lactone efflux protein OS=Pseudoroseicyclus aestuarii OX=1795041 GN=DFP88_104295 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84VRR12 pKa = 11.84KK13 pKa = 9.2ARR15 pKa = 11.84HH16 pKa = 4.71GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.97VLSAA44 pKa = 4.11

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3295

0

3295

1035711

26

2801

314.3

33.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.664 ± 0.079

0.781 ± 0.014

5.77 ± 0.042

6.332 ± 0.045

3.289 ± 0.03

9.311 ± 0.052

1.877 ± 0.023

4.551 ± 0.032

2.116 ± 0.038

10.68 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.598 ± 0.021

1.972 ± 0.025

5.599 ± 0.039

3.304 ± 0.025

7.507 ± 0.048

4.846 ± 0.032

5.269 ± 0.031

7.037 ± 0.033

1.452 ± 0.02

2.045 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski