Limisphaera ngatamarikiensis

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Verrucomicrobiae; Verrucomicrobiales; Verrucomicrobia subdivision 3; Limisphaera

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2810 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M1RDE8|A0A6M1RDE8_9BACT Ferrous iron transport protein A OS=Limisphaera ngatamarikiensis OX=1324935 GN=G4L39_01845 PE=4 SV=1
MM1 pKa = 7.76SYY3 pKa = 10.14QVHH6 pKa = 6.45RR7 pKa = 11.84FLSLSTLLVGLWVVGYY23 pKa = 7.73PSRR26 pKa = 11.84AGEE29 pKa = 4.18VALAWDD35 pKa = 4.88PSPDD39 pKa = 3.17AWVTGYY45 pKa = 10.66AIHH48 pKa = 6.69YY49 pKa = 6.67GTSSGIHH56 pKa = 5.7PVRR59 pKa = 11.84VDD61 pKa = 3.15VGNVTNAIIDD71 pKa = 3.78GLQPGVTYY79 pKa = 10.22YY80 pKa = 10.29FVATAYY86 pKa = 8.78TADD89 pKa = 4.02GLEE92 pKa = 4.29SDD94 pKa = 4.36PSNEE98 pKa = 3.53VSYY101 pKa = 8.86TVPLPQPDD109 pKa = 4.04PTNAPPVVNAGPDD122 pKa = 3.2QTIRR126 pKa = 11.84LPAPALLAGQASDD139 pKa = 4.95DD140 pKa = 4.47GLPADD145 pKa = 4.84PGQLTVWWEE154 pKa = 4.14LVSGPAPVTWSTTNNLTVQVNFTQPGLYY182 pKa = 10.21LFRR185 pKa = 11.84LVASDD190 pKa = 4.03GALSASDD197 pKa = 3.52EE198 pKa = 4.34VQVQVEE204 pKa = 4.46APLPINQPPMVNAGPDD220 pKa = 3.17QTIRR224 pKa = 11.84LPAPALLAGQASDD237 pKa = 4.95DD238 pKa = 4.47GLPADD243 pKa = 4.84PGQLTVWWEE252 pKa = 4.25MVSGPAPVSWSTTNN266 pKa = 3.06

Molecular weight: 28.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M1RS36|A0A6M1RS36_9BACT Uncharacterized protein (Fragment) OS=Limisphaera ngatamarikiensis OX=1324935 GN=G4L39_01810 PE=4 SV=1
MM1 pKa = 7.58GSLKK5 pKa = 10.12KK6 pKa = 10.09RR7 pKa = 11.84RR8 pKa = 11.84KK9 pKa = 9.95AKK11 pKa = 9.7INKK14 pKa = 8.46HH15 pKa = 4.27KK16 pKa = 10.21RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 7.61KK20 pKa = 10.27RR21 pKa = 11.84RR22 pKa = 11.84MHH24 pKa = 5.52RR25 pKa = 11.84HH26 pKa = 6.23KK27 pKa = 10.79KK28 pKa = 8.91RR29 pKa = 11.84TWQTT33 pKa = 2.84

Molecular weight: 4.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2810

0

2810

1068520

33

6189

380.3

42.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.477 ± 0.046

1.121 ± 0.016

4.767 ± 0.035

6.171 ± 0.047

3.479 ± 0.029

8.651 ± 0.054

2.222 ± 0.021

3.88 ± 0.038

2.222 ± 0.034

10.905 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.78 ± 0.021

2.672 ± 0.038

6.551 ± 0.041

3.649 ± 0.028

8.842 ± 0.059

4.461 ± 0.029

5.409 ± 0.044

8.093 ± 0.04

2.22 ± 0.03

2.427 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski