Capybara microvirus Cap1_SP_216

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 5.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W4M2|A0A4P8W4M2_9VIRU Replication initiator protein OS=Capybara microvirus Cap1_SP_216 OX=2585411 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.33KK3 pKa = 10.42LYY5 pKa = 9.56TIHH8 pKa = 7.64DD9 pKa = 4.63KK10 pKa = 10.77IANKK14 pKa = 10.5CGDD17 pKa = 3.32IFTAEE22 pKa = 3.88NDD24 pKa = 3.64KK25 pKa = 10.97IAMRR29 pKa = 11.84NCIHH33 pKa = 6.67MSSKK37 pKa = 10.12MDD39 pKa = 3.38DD40 pKa = 3.42WLKK43 pKa = 10.18TDD45 pKa = 3.62YY46 pKa = 9.72EE47 pKa = 4.43LCVFPIEE54 pKa = 4.69FYY56 pKa = 11.34DD57 pKa = 4.24DD58 pKa = 3.82FGHH61 pKa = 6.89FAVLHH66 pKa = 6.14DD67 pKa = 4.24LCKK70 pKa = 11.06GNIIPYY76 pKa = 10.15DD77 pKa = 3.73VICNFSEE84 pKa = 5.44LSDD87 pKa = 3.53KK88 pKa = 11.37YY89 pKa = 11.56NLVEE93 pKa = 4.96LNEE96 pKa = 4.12QTEE99 pKa = 4.39DD100 pKa = 3.2

Molecular weight: 11.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W5F8|A0A4P8W5F8_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_216 OX=2585411 PE=3 SV=1
MM1 pKa = 7.63ALGFNCLDD9 pKa = 3.84CFHH12 pKa = 6.87TRR14 pKa = 11.84TIKK17 pKa = 10.83RR18 pKa = 11.84PDD20 pKa = 3.46NGSPMVVPCGNCMACRR36 pKa = 11.84IARR39 pKa = 11.84SRR41 pKa = 11.84EE42 pKa = 3.32WSLRR46 pKa = 11.84LLMEE50 pKa = 5.05LEE52 pKa = 3.91SHH54 pKa = 6.8DD55 pKa = 4.36FKK57 pKa = 11.71GCFITLTYY65 pKa = 10.92DD66 pKa = 4.76DD67 pKa = 4.14EE68 pKa = 5.02HH69 pKa = 8.22LPNDD73 pKa = 3.81YY74 pKa = 10.93GLHH77 pKa = 6.63KK78 pKa = 10.65EE79 pKa = 4.08DD80 pKa = 5.77LKK82 pKa = 11.56NFWKK86 pKa = 10.68RR87 pKa = 11.84LRR89 pKa = 11.84KK90 pKa = 10.1DD91 pKa = 2.96LDD93 pKa = 3.75FKK95 pKa = 10.87IKK97 pKa = 10.58YY98 pKa = 6.86YY99 pKa = 11.17ACGEE103 pKa = 4.11YY104 pKa = 10.53GDD106 pKa = 4.28TYY108 pKa = 11.23GRR110 pKa = 11.84PHH112 pKa = 5.37YY113 pKa = 10.04HH114 pKa = 7.1AIIFGLDD121 pKa = 3.11PDD123 pKa = 4.34DD124 pKa = 3.44FTRR127 pKa = 11.84RR128 pKa = 11.84LLVDD132 pKa = 3.14NWRR135 pKa = 11.84FCSPDD140 pKa = 2.99RR141 pKa = 11.84FNGTKK146 pKa = 9.88QGLADD151 pKa = 3.69VTIDD155 pKa = 3.44SINYY159 pKa = 4.03VTGYY163 pKa = 9.15IQKK166 pKa = 10.33KK167 pKa = 9.43YY168 pKa = 10.91NGDD171 pKa = 3.69LAKK174 pKa = 10.21EE175 pKa = 4.58VYY177 pKa = 10.67GEE179 pKa = 4.01LQPPFSSSSQGLGLEE194 pKa = 4.24TFLKK198 pKa = 10.22NRR200 pKa = 11.84EE201 pKa = 3.86RR202 pKa = 11.84FEE204 pKa = 4.18NDD206 pKa = 3.11GYY208 pKa = 10.65IGYY211 pKa = 9.77QGVKK215 pKa = 7.76YY216 pKa = 8.3TIPRR220 pKa = 11.84YY221 pKa = 9.2FIKK224 pKa = 10.69KK225 pKa = 9.94LDD227 pKa = 3.81LEE229 pKa = 4.43VSKK232 pKa = 11.08VYY234 pKa = 9.6STEE237 pKa = 3.75KK238 pKa = 9.56VNEE241 pKa = 3.95YY242 pKa = 10.62LEE244 pKa = 4.57KK245 pKa = 11.15NGIDD249 pKa = 3.23PSRR252 pKa = 11.84FVKK255 pKa = 9.61ATSPNFIKK263 pKa = 10.66NVAYY267 pKa = 10.58HH268 pKa = 6.31EE269 pKa = 4.41VADD272 pKa = 3.8PHH274 pKa = 6.67LAIAEE279 pKa = 4.05RR280 pKa = 11.84RR281 pKa = 11.84LLRR284 pKa = 11.84QKK286 pKa = 10.66QLFEE290 pKa = 4.29KK291 pKa = 10.65DD292 pKa = 3.0KK293 pKa = 11.56LKK295 pKa = 11.15

Molecular weight: 34.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1406

74

559

234.3

26.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.117 ± 1.386

1.351 ± 0.539

6.472 ± 0.97

6.615 ± 0.808

5.334 ± 0.565

6.686 ± 0.732

2.134 ± 0.259

5.69 ± 0.628

6.401 ± 0.905

8.606 ± 0.328

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.418 ± 0.429

7.183 ± 0.688

3.698 ± 0.653

3.698 ± 0.775

4.41 ± 0.866

6.828 ± 0.888

4.836 ± 0.448

5.548 ± 0.369

1.138 ± 0.26

4.836 ± 0.415
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski