Clostridium sp. CAG:354

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1707 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7F753|R7F753_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:354 OX=1262799 GN=BN623_01664 PE=4 SV=1
MM1 pKa = 7.75RR2 pKa = 11.84KK3 pKa = 9.76KK4 pKa = 10.27ILVVVMSLAVLMCGGVVVVAGTDD27 pKa = 3.53PEE29 pKa = 4.44SPEE32 pKa = 4.32TISDD36 pKa = 3.43NSASGTSVDD45 pKa = 3.83EE46 pKa = 4.23YY47 pKa = 11.42EE48 pKa = 4.41MYY50 pKa = 10.8LVRR53 pKa = 11.84DD54 pKa = 3.88NPDD57 pKa = 2.95LCVYY61 pKa = 10.57SMRR64 pKa = 11.84PNKK67 pKa = 9.31TLLLIGSSDD76 pKa = 3.19MANYY80 pKa = 7.74ITYY83 pKa = 11.0ANVNTDD89 pKa = 3.91EE90 pKa = 4.3LTGTPVALADD100 pKa = 4.08DD101 pKa = 4.94FSAVVIEE108 pKa = 4.34NEE110 pKa = 3.89DD111 pKa = 3.48GKK113 pKa = 11.17FVVSTAEE120 pKa = 3.95GEE122 pKa = 3.97EE123 pKa = 4.48AYY125 pKa = 9.92PEE127 pKa = 4.48EE128 pKa = 5.46GPVITPFQEE137 pKa = 4.23FEE139 pKa = 5.25KK140 pKa = 10.49SVEE143 pKa = 4.06WNEE146 pKa = 3.96LYY148 pKa = 10.93YY149 pKa = 11.19

Molecular weight: 16.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7F7D1|R7F7D1_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:354 OX=1262799 GN=BN623_00108 PE=4 SV=1
MM1 pKa = 7.35ARR3 pKa = 11.84ISGIDD8 pKa = 3.44LPRR11 pKa = 11.84EE12 pKa = 3.69KK13 pKa = 10.1RR14 pKa = 11.84VEE16 pKa = 3.92VGLTYY21 pKa = 10.0IYY23 pKa = 10.71GIGLSTSQKK32 pKa = 9.73LLKK35 pKa = 9.77EE36 pKa = 4.21AEE38 pKa = 4.45VNPDD42 pKa = 3.15TRR44 pKa = 11.84VKK46 pKa = 10.84DD47 pKa = 3.56LTEE50 pKa = 4.59DD51 pKa = 3.13QVQAIRR57 pKa = 11.84KK58 pKa = 9.41AIDD61 pKa = 3.44SNHH64 pKa = 6.54LLLEE68 pKa = 4.48GDD70 pKa = 3.93LRR72 pKa = 11.84RR73 pKa = 11.84EE74 pKa = 3.55VALNIKK80 pKa = 9.97RR81 pKa = 11.84LTEE84 pKa = 3.76IGCYY88 pKa = 9.29RR89 pKa = 11.84GLRR92 pKa = 11.84HH93 pKa = 6.72RR94 pKa = 11.84RR95 pKa = 11.84GLPVRR100 pKa = 11.84GQRR103 pKa = 11.84TKK105 pKa = 10.45TNARR109 pKa = 11.84TRR111 pKa = 11.84KK112 pKa = 9.44GPRR115 pKa = 11.84KK116 pKa = 9.46LVSKK120 pKa = 10.76KK121 pKa = 10.17KK122 pKa = 10.43

Molecular weight: 13.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1707

0

1707

476769

29

2892

279.3

31.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.212 ± 0.056

1.055 ± 0.022

5.694 ± 0.053

8.403 ± 0.071

4.001 ± 0.045

5.58 ± 0.063

1.214 ± 0.022

10.157 ± 0.074

9.922 ± 0.072

8.56 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.398 ± 0.028

7.144 ± 0.065

2.555 ± 0.03

2.713 ± 0.027

3.283 ± 0.04

5.507 ± 0.047

5.476 ± 0.064

5.942 ± 0.053

0.647 ± 0.018

4.533 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski