Gavia stellata (Red-throated diver) (Colymbus stellatus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7730 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A093FCD2|A0A093FCD2_GAVST Striated muscle-specific serine/threonine-protein kinase (Fragment) OS=Gavia stellata OX=37040 GN=N328_05227 PE=4 SV=1
MM1 pKa = 6.61TTGDD5 pKa = 3.72VLDD8 pKa = 4.14SRR10 pKa = 11.84TAAEE14 pKa = 4.4LRR16 pKa = 11.84LDD18 pKa = 4.02SLYY21 pKa = 10.39LQCMDD26 pKa = 3.28NCKK29 pKa = 10.62ADD31 pKa = 3.62VSDD34 pKa = 4.11VLPKK38 pKa = 10.28QEE40 pKa = 4.23IQSSEE45 pKa = 4.06CQAAASDD52 pKa = 4.2AEE54 pKa = 4.42VLHH57 pKa = 6.03TARR60 pKa = 11.84GHH62 pKa = 4.77YY63 pKa = 6.39TTPEE67 pKa = 3.88VLLSANAPQIDD78 pKa = 3.67ATEE81 pKa = 4.58CEE83 pKa = 4.35VGLADD88 pKa = 4.52TYY90 pKa = 11.44LSPTADD96 pKa = 3.12SCEE99 pKa = 4.25NISLATTDD107 pKa = 4.35KK108 pKa = 11.42GNLPHH113 pKa = 7.14SIVYY117 pKa = 10.03QNEE120 pKa = 3.61EE121 pKa = 4.41GKK123 pKa = 9.57WVTDD127 pKa = 3.51LAYY130 pKa = 9.07YY131 pKa = 10.17TSFDD135 pKa = 3.7EE136 pKa = 4.54EE137 pKa = 3.83QDD139 pKa = 3.52INLSEE144 pKa = 4.41DD145 pKa = 3.39DD146 pKa = 4.69KK147 pKa = 11.6INEE150 pKa = 4.01EE151 pKa = 4.77FITT154 pKa = 4.04

Molecular weight: 16.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A093HCC6|A0A093HCC6_GAVST Membrane progestin receptor beta OS=Gavia stellata OX=37040 GN=N328_12892 PE=3 SV=1
KKK2 pKa = 10.12RR3 pKa = 11.84QGKKK7 pKa = 8.85RR8 pKa = 11.84QGKKK12 pKa = 8.73RR13 pKa = 11.84QGKKK17 pKa = 8.73RR18 pKa = 11.84QGKKK22 pKa = 8.73RR23 pKa = 11.84QGKKK27 pKa = 8.73RR28 pKa = 11.84QGKKK32 pKa = 8.73RR33 pKa = 11.84QGKKK37 pKa = 8.73RR38 pKa = 11.84QGKKK42 pKa = 8.73RR43 pKa = 11.84QGKKK47 pKa = 8.73RR48 pKa = 11.84QGKKK52 pKa = 8.73RR53 pKa = 11.84QGKKK57 pKa = 9.02RR58 pKa = 11.84QSKKK62 pKa = 10.33K

Molecular weight: 6.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7730

0

7730

3192604

33

4973

413.0

46.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.319 ± 0.023

2.218 ± 0.024

5.037 ± 0.019

7.213 ± 0.033

3.956 ± 0.02

5.74 ± 0.033

2.529 ± 0.011

5.112 ± 0.019

6.563 ± 0.033

9.706 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.276 ± 0.012

4.228 ± 0.019

5.195 ± 0.034

4.682 ± 0.025

5.121 ± 0.019

8.167 ± 0.038

5.44 ± 0.02

6.296 ± 0.023

1.175 ± 0.01

3.021 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski