Pedobacter steynii

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Pedobacter

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5356 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D7QM25|A0A1D7QM25_9SPHI Uncharacterized protein OS=Pedobacter steynii OX=430522 GN=BFS30_22390 PE=4 SV=1
MM1 pKa = 7.31PQLKK5 pKa = 10.36AGEE8 pKa = 4.23TANITFTFSEE18 pKa = 4.62DD19 pKa = 3.31PGTTFAWDD27 pKa = 3.46GTTGDD32 pKa = 3.93VVVSGGTLGAISGSGLTRR50 pKa = 11.84TATFTPTPASSGTASITVAAATYY73 pKa = 9.97TDD75 pKa = 3.34AAGNDD80 pKa = 3.96GGAGTTPALTFDD92 pKa = 4.01TQSPNAPSAPVLAAASDD109 pKa = 3.96SGISNSDD116 pKa = 3.56NITNVTTPVFTGTAEE131 pKa = 4.32PGSTVTLYY139 pKa = 9.67DD140 pKa = 3.39TDD142 pKa = 3.55GTTVIGTVLLPVEE155 pKa = 4.73TGQLQQDD162 pKa = 2.9IDD164 pKa = 3.88TRR166 pKa = 11.84HH167 pKa = 5.76TYY169 pKa = 10.2HH170 pKa = 6.78YY171 pKa = 10.96GG172 pKa = 3.22

Molecular weight: 17.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D7QIB4|A0A1D7QIB4_9SPHI ATP phosphoribosyltransferase OS=Pedobacter steynii OX=430522 GN=hisG PE=3 SV=1
MM1 pKa = 7.4NNIIHH6 pKa = 6.61IFVLVGFLLNCFTRR20 pKa = 11.84IVKK23 pKa = 10.47GSTQSFQSSDD33 pKa = 2.96RR34 pKa = 11.84HH35 pKa = 6.36RR36 pKa = 11.84GFCFYY41 pKa = 10.65IRR43 pKa = 11.84VALLRR48 pKa = 11.84LGADD52 pKa = 3.23VSVDD56 pKa = 3.94FFHH59 pKa = 7.5LVGLVRR65 pKa = 11.84FKK67 pKa = 10.7IGFVVFIFSQQ77 pKa = 3.23

Molecular weight: 8.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5356

0

5356

1988063

36

6653

371.2

41.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.326 ± 0.037

0.734 ± 0.01

5.215 ± 0.023

5.901 ± 0.042

4.907 ± 0.025

6.935 ± 0.034

1.775 ± 0.018

7.131 ± 0.03

7.148 ± 0.037

9.76 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.271 ± 0.016

5.626 ± 0.034

3.829 ± 0.018

3.709 ± 0.021

3.906 ± 0.024

6.682 ± 0.033

5.675 ± 0.059

6.159 ± 0.025

1.18 ± 0.013

4.132 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski