Tolypothrix sp. NIES-4075

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Tolypothrichaceae; Tolypothrix; unclassified Tolypothrix

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7285 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A218QB29|A0A218QB29_9CYAN DUF3598 domain-containing protein OS=Tolypothrix sp. NIES-4075 OX=2005459 GN=NIES4075_00490 PE=3 SV=1
MM1 pKa = 7.47ALEE4 pKa = 3.99FDD6 pKa = 4.17YY7 pKa = 11.4NEE9 pKa = 4.1EE10 pKa = 3.85EE11 pKa = 4.29IYY13 pKa = 10.64PPFTGTEE20 pKa = 4.05FVANNEE26 pKa = 4.67TIDD29 pKa = 3.49SHH31 pKa = 6.81PYY33 pKa = 9.8AVAFGQFLGSGIHH46 pKa = 7.26PDD48 pKa = 3.53TANEE52 pKa = 4.03LAFGLHH58 pKa = 5.83SAISQISDD66 pKa = 3.48DD67 pKa = 4.38PFDD70 pKa = 3.93EE71 pKa = 4.28LRR73 pKa = 11.84VRR75 pKa = 11.84AVNIFDD81 pKa = 4.74RR82 pKa = 11.84LNEE85 pKa = 3.76MNYY88 pKa = 9.28WEE90 pKa = 4.87FSSSEE95 pKa = 4.38DD96 pKa = 4.7GDD98 pKa = 3.95HH99 pKa = 6.8QPDD102 pKa = 3.12NFVFTEE108 pKa = 4.02

Molecular weight: 12.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A218QQI8|A0A218QQI8_9CYAN Twitching motility protein PilT OS=Tolypothrix sp. NIES-4075 OX=2005459 GN=pilT_2 PE=3 SV=1
MM1 pKa = 7.68RR2 pKa = 11.84VILKK6 pKa = 9.85RR7 pKa = 11.84DD8 pKa = 3.22RR9 pKa = 11.84LLLFRR14 pKa = 11.84LVQNGGNKK22 pKa = 8.45ATISNTQKK30 pKa = 10.3PCISSFLNFADD41 pKa = 3.12IVVANATVNHH51 pKa = 6.96CDD53 pKa = 3.33DD54 pKa = 3.31MGRR57 pKa = 11.84ILRR60 pKa = 11.84EE61 pKa = 3.63AARR64 pKa = 11.84LVRR67 pKa = 11.84PGGLLFTDD75 pKa = 3.74QDD77 pKa = 4.08PQRR80 pKa = 11.84SAWNFKK86 pKa = 10.61GFGLFLRR93 pKa = 11.84DD94 pKa = 2.83IRR96 pKa = 11.84YY97 pKa = 10.1SFMCIARR104 pKa = 11.84RR105 pKa = 11.84NYY107 pKa = 10.37

Molecular weight: 12.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7285

0

7285

2192051

29

4039

300.9

33.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.092 ± 0.035

1.025 ± 0.01

4.823 ± 0.02

6.185 ± 0.031

3.989 ± 0.022

6.506 ± 0.034

1.806 ± 0.013

6.77 ± 0.024

5.206 ± 0.03

10.882 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.857 ± 0.014

4.573 ± 0.022

4.609 ± 0.022

5.266 ± 0.028

5.072 ± 0.021

6.608 ± 0.027

5.673 ± 0.025

6.598 ± 0.025

1.391 ± 0.013

3.068 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski