Beet black scorch virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Betanecrovirus

Average proteome isoelectric point is 8.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8B5S0|Q8B5S0_9TOMB RNA-directed RNA polymerase OS=Beet black scorch virus OX=196375 PE=3 SV=2
MM1 pKa = 8.0DD2 pKa = 6.13SIPYY6 pKa = 9.68VILRR10 pKa = 11.84ILDD13 pKa = 4.46FIFHH17 pKa = 6.86SIFFPSLLFIINHH30 pKa = 5.43NTTILWACACAYY42 pKa = 10.09GFYY45 pKa = 10.28RR46 pKa = 11.84AFRR49 pKa = 11.84LIFKK53 pKa = 10.11IKK55 pKa = 10.72VEE57 pKa = 3.86VHH59 pKa = 5.58PATRR63 pKa = 11.84AVFKK67 pKa = 11.36DD68 pKa = 3.05MVTRR72 pKa = 11.84FQRR75 pKa = 11.84EE76 pKa = 4.41SMFSPDD82 pKa = 3.74DD83 pKa = 3.81EE84 pKa = 4.64VPEE87 pKa = 4.98GIPIHH92 pKa = 6.76EE93 pKa = 5.03DD94 pKa = 2.76VDD96 pKa = 4.42LVSDD100 pKa = 4.26PTHH103 pKa = 6.86KK104 pKa = 10.55DD105 pKa = 2.72IKK107 pKa = 10.13RR108 pKa = 11.84VRR110 pKa = 11.84ASRR113 pKa = 11.84RR114 pKa = 11.84VSYY117 pKa = 10.44AVRR120 pKa = 11.84VAHH123 pKa = 5.49VAKK126 pKa = 10.72SKK128 pKa = 11.07VGLLANTKK136 pKa = 10.39ANEE139 pKa = 4.02LVYY142 pKa = 10.99SRR144 pKa = 11.84LCRR147 pKa = 11.84DD148 pKa = 3.16EE149 pKa = 4.24MVTHH153 pKa = 6.71GVRR156 pKa = 11.84PSHH159 pKa = 6.84IAHH162 pKa = 7.05AVPLAVAACFIPLDD176 pKa = 3.96SDD178 pKa = 3.7FLAASIRR185 pKa = 11.84NCDD188 pKa = 3.22EE189 pKa = 3.71MEE191 pKa = 3.87EE192 pKa = 4.05RR193 pKa = 11.84RR194 pKa = 11.84AVLGPSYY201 pKa = 11.22GKK203 pKa = 10.5

Molecular weight: 23.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8B5R9|Q8B5R9_9TOMB RNA-directed RNA polymerase OS=Beet black scorch virus OX=196375 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.32NRR3 pKa = 11.84LPWRR7 pKa = 11.84MGFLVSALISWTRR20 pKa = 11.84TPRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84SASSSSLGHH34 pKa = 6.28EE35 pKa = 4.3NLTTFHH41 pKa = 6.45GTTAA45 pKa = 3.56

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1332

45

724

222.0

24.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.408 ± 1.31

1.652 ± 0.352

4.73 ± 0.794

5.03 ± 0.739

4.955 ± 0.631

6.682 ± 0.824

2.853 ± 0.651

5.405 ± 0.958

4.279 ± 0.48

7.883 ± 0.986

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.928 ± 0.282

3.829 ± 0.575

5.105 ± 0.409

2.853 ± 0.677

7.733 ± 0.644

7.432 ± 1.143

5.631 ± 1.324

8.183 ± 0.642

1.201 ± 0.261

3.153 ± 0.29
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski