Aeromonas phage 62AhydR11PP

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1PDK6|A0A2S1PDK6_9CAUD Uncharacterized protein OS=Aeromonas phage 62AhydR11PP OX=2163963 PE=4 SV=1
MM1 pKa = 7.72DD2 pKa = 4.14TMFLNPTTWDD12 pKa = 3.61LDD14 pKa = 3.44VDD16 pKa = 4.03ASGSIALSSSAYY28 pKa = 10.44SVAQDD33 pKa = 3.4VASQSLLWRR42 pKa = 11.84GEE44 pKa = 3.87APYY47 pKa = 9.2NTGDD51 pKa = 4.3GIPYY55 pKa = 9.46EE56 pKa = 4.21EE57 pKa = 4.67SVLGQRR63 pKa = 11.84PAQATLAAWYY73 pKa = 8.63QQEE76 pKa = 4.22ALRR79 pKa = 11.84VPDD82 pKa = 3.69VASATPVLIYY92 pKa = 10.61DD93 pKa = 3.51QARR96 pKa = 11.84GVTGQIQVTLTDD108 pKa = 3.54GTSINVV114 pKa = 3.2

Molecular weight: 12.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1PDN0|A0A2S1PDN0_9CAUD Uncharacterized protein OS=Aeromonas phage 62AhydR11PP OX=2163963 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.4KK3 pKa = 8.73PVKK6 pKa = 9.75MHH8 pKa = 4.86GHH10 pKa = 5.86FLKK13 pKa = 11.0GSDD16 pKa = 3.61MQNTRR21 pKa = 11.84HH22 pKa = 5.73TKK24 pKa = 9.97SEE26 pKa = 3.75ARR28 pKa = 11.84SIAVRR33 pKa = 11.84SAKK36 pKa = 10.17RR37 pKa = 11.84AGVWPLVIEE46 pKa = 4.31RR47 pKa = 11.84FDD49 pKa = 3.69AGEE52 pKa = 4.19HH53 pKa = 5.14YY54 pKa = 10.63VVSSHH59 pKa = 5.81TVGHH63 pKa = 6.56RR64 pKa = 11.84PP65 pKa = 3.28

Molecular weight: 7.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

13421

52

854

209.7

23.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.08 ± 0.48

1.334 ± 0.144

5.991 ± 0.237

6.184 ± 0.352

3.517 ± 0.201

7.861 ± 0.313

1.699 ± 0.185

5.313 ± 0.164

5.149 ± 0.346

7.473 ± 0.289

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.891 ± 0.162

3.726 ± 0.201

4.486 ± 0.223

4.299 ± 0.218

5.67 ± 0.267

6.162 ± 0.25

5.7 ± 0.289

6.915 ± 0.272

1.676 ± 0.148

2.876 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski