Cryobacterium sp. Hh4

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Cryobacterium; unclassified Cryobacterium

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3130 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R9AZC9|A0A4R9AZC9_9MICO VOC family protein OS=Cryobacterium sp. Hh4 OX=1259157 GN=E3T48_14795 PE=4 SV=1
MM1 pKa = 7.2FGVASFYY8 pKa = 11.38AVLGVPMSARR18 pKa = 11.84SWLITSALTVAAVTLVGCAQPTPEE42 pKa = 4.34PSVTPVAEE50 pKa = 4.15PVHH53 pKa = 5.92WSYY56 pKa = 11.99DD57 pKa = 3.64GASGPASWAEE67 pKa = 3.63LDD69 pKa = 4.82SSFEE73 pKa = 4.15TCAVGTDD80 pKa = 3.6QSPIDD85 pKa = 4.2LPATVPAPSTSIRR98 pKa = 11.84LSAEE102 pKa = 3.96DD103 pKa = 3.77ADD105 pKa = 4.37GDD107 pKa = 4.47VFDD110 pKa = 4.9TGHH113 pKa = 6.2AVEE116 pKa = 5.24FEE118 pKa = 4.16SDD120 pKa = 3.8GEE122 pKa = 4.59GEE124 pKa = 4.43TLTFAGDD131 pKa = 3.83EE132 pKa = 4.23YY133 pKa = 11.5SLQQMHH139 pKa = 6.24AHH141 pKa = 5.95VPSEE145 pKa = 4.14HH146 pKa = 5.99TVNGQPAAAEE156 pKa = 3.99LHH158 pKa = 6.39LVHH161 pKa = 7.19ADD163 pKa = 3.0ADD165 pKa = 3.86GRR167 pKa = 11.84LLVLGILVTEE177 pKa = 5.03GQASDD182 pKa = 3.48ALMPFIEE189 pKa = 4.85AATHH193 pKa = 5.61VADD196 pKa = 4.53EE197 pKa = 4.81EE198 pKa = 4.5DD199 pKa = 3.37VTMDD203 pKa = 3.17VSAVLPPSLEE213 pKa = 3.94NYY215 pKa = 8.29EE216 pKa = 4.14YY217 pKa = 10.78SGSLTTPPCTEE228 pKa = 3.83DD229 pKa = 3.57VQWVVMSTPISMSAEE244 pKa = 3.82QIGTLEE250 pKa = 4.49GAHH253 pKa = 5.85SHH255 pKa = 5.76NARR258 pKa = 11.84PTQPLGDD265 pKa = 3.37RR266 pKa = 11.84VVVGGTADD274 pKa = 4.98LDD276 pKa = 3.98VEE278 pKa = 4.54DD279 pKa = 4.51

Molecular weight: 29.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R9B4C3|A0A4R9B4C3_9MICO CbiA domain-containing protein OS=Cryobacterium sp. Hh4 OX=1259157 GN=E3T48_11555 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3130

0

3130

988464

30

1966

315.8

33.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.127 ± 0.06

0.558 ± 0.011

5.868 ± 0.036

5.211 ± 0.047

3.244 ± 0.029

9.052 ± 0.042

1.966 ± 0.019

4.708 ± 0.035

2.25 ± 0.034

10.513 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.806 ± 0.016

2.241 ± 0.023

5.367 ± 0.033

2.805 ± 0.023

7.058 ± 0.049

5.829 ± 0.027

6.201 ± 0.038

8.783 ± 0.044

1.407 ± 0.016

2.006 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski