Amino acid dipepetide frequency for Salmonella phage SPN3US

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.235AlaAla: 7.235 ± 0.38
0.72AlaCys: 0.72 ± 0.108
4.823AlaAsp: 4.823 ± 0.251
4.957AlaGlu: 4.957 ± 0.278
3.158AlaPhe: 3.158 ± 0.209
4.903AlaGly: 4.903 ± 0.28
1.412AlaHis: 1.412 ± 0.115
5.09AlaIle: 5.09 ± 0.225
4.21AlaLys: 4.21 ± 0.287
7.222AlaLeu: 7.222 ± 0.325
2.265AlaMet: 2.265 ± 0.155
3.398AlaAsn: 3.398 ± 0.217
3.118AlaPro: 3.118 ± 0.248
2.558AlaGln: 2.558 ± 0.188
3.504AlaArg: 3.504 ± 0.218
3.957AlaSer: 3.957 ± 0.242
5.023AlaThr: 5.023 ± 0.293
5.569AlaVal: 5.569 ± 0.281
1.186AlaTrp: 1.186 ± 0.126
3.011AlaTyr: 3.011 ± 0.201
0.0AlaXaa: 0.0 ± 0.0
Cys
0.573CysAla: 0.573 ± 0.096
0.12CysCys: 0.12 ± 0.041
0.506CysAsp: 0.506 ± 0.069
0.6CysGlu: 0.6 ± 0.084
0.2CysPhe: 0.2 ± 0.042
0.746CysGly: 0.746 ± 0.112
0.306CysHis: 0.306 ± 0.064
0.413CysIle: 0.413 ± 0.079
0.386CysLys: 0.386 ± 0.084
0.839CysLeu: 0.839 ± 0.098
0.173CysMet: 0.173 ± 0.05
0.533CysAsn: 0.533 ± 0.098
0.36CysPro: 0.36 ± 0.065
0.386CysGln: 0.386 ± 0.077
0.6CysArg: 0.6 ± 0.104
0.626CysSer: 0.626 ± 0.078
0.56CysThr: 0.56 ± 0.092
0.666CysVal: 0.666 ± 0.103
0.133CysTrp: 0.133 ± 0.041
0.453CysTyr: 0.453 ± 0.077
0.0CysXaa: 0.0 ± 0.0
Asp
5.037AspAla: 5.037 ± 0.293
0.6AspCys: 0.6 ± 0.1
3.811AspAsp: 3.811 ± 0.236
4.477AspGlu: 4.477 ± 0.296
2.505AspPhe: 2.505 ± 0.177
4.837AspGly: 4.837 ± 0.27
1.039AspHis: 1.039 ± 0.115
3.824AspIle: 3.824 ± 0.229
2.985AspLys: 2.985 ± 0.228
5.516AspLeu: 5.516 ± 0.289
1.612AspMet: 1.612 ± 0.145
2.758AspAsn: 2.758 ± 0.213
3.238AspPro: 3.238 ± 0.205
1.759AspGln: 1.759 ± 0.16
3.211AspArg: 3.211 ± 0.244
3.025AspSer: 3.025 ± 0.206
3.877AspThr: 3.877 ± 0.193
4.943AspVal: 4.943 ± 0.251
1.133AspTrp: 1.133 ± 0.125
2.705AspTyr: 2.705 ± 0.185
0.0AspXaa: 0.0 ± 0.0
Glu
4.77GluAla: 4.77 ± 0.25
0.6GluCys: 0.6 ± 0.093
3.957GluAsp: 3.957 ± 0.233
4.104GluGlu: 4.104 ± 0.307
2.771GluPhe: 2.771 ± 0.221
3.931GluGly: 3.931 ± 0.222
1.319GluHis: 1.319 ± 0.145
3.984GluIle: 3.984 ± 0.244
3.571GluLys: 3.571 ± 0.251
7.048GluLeu: 7.048 ± 0.286
1.945GluMet: 1.945 ± 0.168
2.598GluAsn: 2.598 ± 0.201
2.345GluPro: 2.345 ± 0.19
2.025GluGln: 2.025 ± 0.171
3.797GluArg: 3.797 ± 0.257
3.131GluSer: 3.131 ± 0.182
3.664GluThr: 3.664 ± 0.186
4.317GluVal: 4.317 ± 0.236
1.226GluTrp: 1.226 ± 0.108
2.865GluTyr: 2.865 ± 0.183
0.0GluXaa: 0.0 ± 0.0
Phe
2.558PheAla: 2.558 ± 0.175
0.386PheCys: 0.386 ± 0.071
2.771PheAsp: 2.771 ± 0.175
2.465PheGlu: 2.465 ± 0.185
1.572PhePhe: 1.572 ± 0.156
2.505PheGly: 2.505 ± 0.183
0.799PheHis: 0.799 ± 0.122
1.825PheIle: 1.825 ± 0.148
1.852PheLys: 1.852 ± 0.159
2.985PheLeu: 2.985 ± 0.196
1.093PheMet: 1.093 ± 0.125
2.572PheAsn: 2.572 ± 0.197
1.745PhePro: 1.745 ± 0.158
1.173PheGln: 1.173 ± 0.126
2.052PheArg: 2.052 ± 0.145
2.558PheSer: 2.558 ± 0.207
3.184PheThr: 3.184 ± 0.189
2.825PheVal: 2.825 ± 0.207
0.493PheTrp: 0.493 ± 0.073
1.825PheTyr: 1.825 ± 0.15
0.0PheXaa: 0.0 ± 0.0
Gly
4.011GlyAla: 4.011 ± 0.254
0.586GlyCys: 0.586 ± 0.106
3.877GlyAsp: 3.877 ± 0.217
4.823GlyGlu: 4.823 ± 0.236
3.144GlyPhe: 3.144 ± 0.184
4.663GlyGly: 4.663 ± 0.429
1.199GlyHis: 1.199 ± 0.136
3.771GlyIle: 3.771 ± 0.205
4.144GlyLys: 4.144 ± 0.269
6.156GlyLeu: 6.156 ± 0.297
1.839GlyMet: 1.839 ± 0.144
3.144GlyAsn: 3.144 ± 0.192
1.732GlyPro: 1.732 ± 0.16
2.691GlyGln: 2.691 ± 0.186
3.584GlyArg: 3.584 ± 0.218
3.598GlySer: 3.598 ± 0.241
4.464GlyThr: 4.464 ± 0.299
4.663GlyVal: 4.663 ± 0.249
1.026GlyTrp: 1.026 ± 0.12
2.918GlyTyr: 2.918 ± 0.181
0.0GlyXaa: 0.0 ± 0.0
His
1.412HisAla: 1.412 ± 0.137
0.227HisCys: 0.227 ± 0.046
1.412HisAsp: 1.412 ± 0.131
1.093HisGlu: 1.093 ± 0.107
0.853HisPhe: 0.853 ± 0.11
1.173HisGly: 1.173 ± 0.122
0.573HisHis: 0.573 ± 0.095
1.186HisIle: 1.186 ± 0.115
0.573HisLys: 0.573 ± 0.099
1.799HisLeu: 1.799 ± 0.157
0.493HisMet: 0.493 ± 0.098
0.786HisAsn: 0.786 ± 0.093
1.053HisPro: 1.053 ± 0.113
0.666HisGln: 0.666 ± 0.097
1.346HisArg: 1.346 ± 0.143
0.906HisSer: 0.906 ± 0.097
1.279HisThr: 1.279 ± 0.148
1.386HisVal: 1.386 ± 0.128
0.28HisTrp: 0.28 ± 0.06
1.199HisTyr: 1.199 ± 0.123
0.0HisXaa: 0.0 ± 0.0
Ile
4.304IleAla: 4.304 ± 0.227
0.653IleCys: 0.653 ± 0.078
4.357IleAsp: 4.357 ± 0.248
3.931IleGlu: 3.931 ± 0.235
1.732IlePhe: 1.732 ± 0.144
3.544IleGly: 3.544 ± 0.217
0.906IleHis: 0.906 ± 0.103
2.771IleIle: 2.771 ± 0.173
2.851IleLys: 2.851 ± 0.197
3.957IleLeu: 3.957 ± 0.225
1.079IleMet: 1.079 ± 0.11
3.091IleAsn: 3.091 ± 0.219
3.291IlePro: 3.291 ± 0.207
1.679IleGln: 1.679 ± 0.155
3.637IleArg: 3.637 ± 0.261
3.558IleSer: 3.558 ± 0.22
4.064IleThr: 4.064 ± 0.225
3.731IleVal: 3.731 ± 0.191
0.64IleTrp: 0.64 ± 0.096
2.332IleTyr: 2.332 ± 0.137
0.0IleXaa: 0.0 ± 0.0
Lys
4.29LysAla: 4.29 ± 0.313
0.413LysCys: 0.413 ± 0.066
2.971LysAsp: 2.971 ± 0.239
3.624LysGlu: 3.624 ± 0.269
1.892LysPhe: 1.892 ± 0.167
3.118LysGly: 3.118 ± 0.216
1.106LysHis: 1.106 ± 0.135
2.705LysIle: 2.705 ± 0.184
2.638LysLys: 2.638 ± 0.223
5.663LysLeu: 5.663 ± 0.308
1.812LysMet: 1.812 ± 0.147
2.145LysAsn: 2.145 ± 0.16
2.891LysPro: 2.891 ± 0.187
2.225LysGln: 2.225 ± 0.207
3.344LysArg: 3.344 ± 0.243
2.585LysSer: 2.585 ± 0.207
3.238LysThr: 3.238 ± 0.231
3.651LysVal: 3.651 ± 0.23
0.653LysTrp: 0.653 ± 0.083
1.919LysTyr: 1.919 ± 0.164
0.0LysXaa: 0.0 ± 0.0
Leu
7.528LeuAla: 7.528 ± 0.314
0.946LeuCys: 0.946 ± 0.124
5.809LeuAsp: 5.809 ± 0.283
5.303LeuGlu: 5.303 ± 0.297
3.224LeuPhe: 3.224 ± 0.198
5.276LeuGly: 5.276 ± 0.296
1.839LeuHis: 1.839 ± 0.161
4.344LeuIle: 4.344 ± 0.288
4.983LeuLys: 4.983 ± 0.273
7.488LeuLeu: 7.488 ± 0.388
2.198LeuMet: 2.198 ± 0.191
5.17LeuAsn: 5.17 ± 0.26
5.116LeuPro: 5.116 ± 0.251
3.384LeuGln: 3.384 ± 0.21
5.396LeuArg: 5.396 ± 0.277
6.289LeuSer: 6.289 ± 0.307
6.555LeuThr: 6.555 ± 0.34
5.676LeuVal: 5.676 ± 0.313
1.026LeuTrp: 1.026 ± 0.131
3.304LeuTyr: 3.304 ± 0.253
0.0LeuXaa: 0.0 ± 0.0
Met
2.452MetAla: 2.452 ± 0.167
0.333MetCys: 0.333 ± 0.072
1.199MetAsp: 1.199 ± 0.118
1.452MetGlu: 1.452 ± 0.141
1.186MetPhe: 1.186 ± 0.128
1.652MetGly: 1.652 ± 0.133
0.573MetHis: 0.573 ± 0.084
1.466MetIle: 1.466 ± 0.154
1.452MetLys: 1.452 ± 0.15
2.545MetLeu: 2.545 ± 0.204
0.826MetMet: 0.826 ± 0.113
1.252MetAsn: 1.252 ± 0.135
0.986MetPro: 0.986 ± 0.107
0.959MetGln: 0.959 ± 0.107
1.612MetArg: 1.612 ± 0.142
1.932MetSer: 1.932 ± 0.142
1.692MetThr: 1.692 ± 0.138
1.852MetVal: 1.852 ± 0.177
0.293MetTrp: 0.293 ± 0.073
1.039MetTyr: 1.039 ± 0.127
0.0MetXaa: 0.0 ± 0.0
Asn
4.197AsnAla: 4.197 ± 0.238
0.36AsnCys: 0.36 ± 0.08
2.811AsnAsp: 2.811 ± 0.182
2.811AsnGlu: 2.811 ± 0.184
1.705AsnPhe: 1.705 ± 0.138
4.304AsnGly: 4.304 ± 0.305
0.866AsnHis: 0.866 ± 0.097
2.438AsnIle: 2.438 ± 0.167
2.425AsnLys: 2.425 ± 0.179
4.024AsnLeu: 4.024 ± 0.261
1.053AsnMet: 1.053 ± 0.113
2.412AsnAsn: 2.412 ± 0.199
3.065AsnPro: 3.065 ± 0.22
1.666AsnGln: 1.666 ± 0.167
2.598AsnArg: 2.598 ± 0.191
2.518AsnSer: 2.518 ± 0.187
3.025AsnThr: 3.025 ± 0.215
3.318AsnVal: 3.318 ± 0.212
0.746AsnTrp: 0.746 ± 0.111
1.919AsnTyr: 1.919 ± 0.166
0.0AsnXaa: 0.0 ± 0.0
Pro
3.651ProAla: 3.651 ± 0.271
0.2ProCys: 0.2 ± 0.053
3.504ProAsp: 3.504 ± 0.231
3.571ProGlu: 3.571 ± 0.236
1.532ProPhe: 1.532 ± 0.165
3.051ProGly: 3.051 ± 0.218
0.906ProHis: 0.906 ± 0.113
2.758ProIle: 2.758 ± 0.187
2.545ProLys: 2.545 ± 0.184
3.784ProLeu: 3.784 ± 0.222
1.159ProMet: 1.159 ± 0.115
1.972ProAsn: 1.972 ± 0.183
1.799ProPro: 1.799 ± 0.147
1.705ProGln: 1.705 ± 0.164
2.265ProArg: 2.265 ± 0.207
2.678ProSer: 2.678 ± 0.174
3.065ProThr: 3.065 ± 0.215
3.904ProVal: 3.904 ± 0.226
0.64ProTrp: 0.64 ± 0.105
1.839ProTyr: 1.839 ± 0.145
0.0ProXaa: 0.0 ± 0.0
Gln
2.678GlnAla: 2.678 ± 0.198
0.333GlnCys: 0.333 ± 0.074
1.666GlnAsp: 1.666 ± 0.149
2.198GlnGlu: 2.198 ± 0.183
1.666GlnPhe: 1.666 ± 0.156
1.972GlnGly: 1.972 ± 0.194
0.746GlnHis: 0.746 ± 0.105
2.145GlnIle: 2.145 ± 0.148
1.785GlnLys: 1.785 ± 0.154
3.717GlnLeu: 3.717 ± 0.238
1.159GlnMet: 1.159 ± 0.131
1.546GlnAsn: 1.546 ± 0.131
1.426GlnPro: 1.426 ± 0.131
1.919GlnGln: 1.919 ± 0.228
2.452GlnArg: 2.452 ± 0.193
1.759GlnSer: 1.759 ± 0.151
2.185GlnThr: 2.185 ± 0.156
2.305GlnVal: 2.305 ± 0.164
0.613GlnTrp: 0.613 ± 0.076
1.386GlnTyr: 1.386 ± 0.123
0.0GlnXaa: 0.0 ± 0.0
Arg
3.704ArgAla: 3.704 ± 0.257
0.613ArgCys: 0.613 ± 0.083
3.304ArgAsp: 3.304 ± 0.225
3.318ArgGlu: 3.318 ± 0.247
2.518ArgPhe: 2.518 ± 0.183
3.624ArgGly: 3.624 ± 0.239
1.079ArgHis: 1.079 ± 0.123
3.398ArgIle: 3.398 ± 0.22
3.371ArgLys: 3.371 ± 0.241
5.383ArgLeu: 5.383 ± 0.278
1.652ArgMet: 1.652 ± 0.142
2.785ArgAsn: 2.785 ± 0.192
2.198ArgPro: 2.198 ± 0.184
1.985ArgGln: 1.985 ± 0.153
3.478ArgArg: 3.478 ± 0.24
2.785ArgSer: 2.785 ± 0.215
3.171ArgThr: 3.171 ± 0.215
3.518ArgVal: 3.518 ± 0.239
1.146ArgTrp: 1.146 ± 0.152
2.585ArgTyr: 2.585 ± 0.177
0.0ArgXaa: 0.0 ± 0.0
Ser
4.29SerAla: 4.29 ± 0.259
0.44SerCys: 0.44 ± 0.074
3.091SerAsp: 3.091 ± 0.192
3.171SerGlu: 3.171 ± 0.206
2.159SerPhe: 2.159 ± 0.184
4.277SerGly: 4.277 ± 0.251
0.933SerHis: 0.933 ± 0.11
3.451SerIle: 3.451 ± 0.219
3.251SerLys: 3.251 ± 0.22
5.223SerLeu: 5.223 ± 0.275
1.546SerMet: 1.546 ± 0.146
2.505SerAsn: 2.505 ± 0.183
2.612SerPro: 2.612 ± 0.181
2.198SerGln: 2.198 ± 0.157
2.865SerArg: 2.865 ± 0.22
3.371SerSer: 3.371 ± 0.269
3.598SerThr: 3.598 ± 0.248
4.064SerVal: 4.064 ± 0.248
0.786SerTrp: 0.786 ± 0.104
2.012SerTyr: 2.012 ± 0.151
0.0SerXaa: 0.0 ± 0.0
Thr
5.17ThrAla: 5.17 ± 0.289
0.426ThrCys: 0.426 ± 0.083
4.144ThrAsp: 4.144 ± 0.238
4.024ThrGlu: 4.024 ± 0.251
2.878ThrPhe: 2.878 ± 0.23
4.663ThrGly: 4.663 ± 0.276
1.399ThrHis: 1.399 ± 0.114
3.558ThrIle: 3.558 ± 0.257
3.038ThrLys: 3.038 ± 0.194
6.929ThrLeu: 6.929 ± 0.388
1.452ThrMet: 1.452 ± 0.155
2.931ThrAsn: 2.931 ± 0.211
3.717ThrPro: 3.717 ± 0.236
1.879ThrGln: 1.879 ± 0.168
3.025ThrArg: 3.025 ± 0.214
3.598ThrSer: 3.598 ± 0.241
4.61ThrThr: 4.61 ± 0.314
5.17ThrVal: 5.17 ± 0.294
1.053ThrTrp: 1.053 ± 0.102
2.412ThrTyr: 2.412 ± 0.167
0.0ThrXaa: 0.0 ± 0.0
Val
5.649ValAla: 5.649 ± 0.285
0.613ValCys: 0.613 ± 0.094
5.05ValAsp: 5.05 ± 0.268
5.103ValGlu: 5.103 ± 0.306
2.478ValPhe: 2.478 ± 0.175
4.024ValGly: 4.024 ± 0.242
1.226ValHis: 1.226 ± 0.129
4.104ValIle: 4.104 ± 0.213
4.051ValLys: 4.051 ± 0.257
5.436ValLeu: 5.436 ± 0.261
1.745ValMet: 1.745 ± 0.134
3.677ValAsn: 3.677 ± 0.198
3.371ValPro: 3.371 ± 0.201
2.398ValGln: 2.398 ± 0.165
3.611ValArg: 3.611 ± 0.215
3.771ValSer: 3.771 ± 0.293
5.01ValThr: 5.01 ± 0.264
5.809ValVal: 5.809 ± 0.357
0.919ValTrp: 0.919 ± 0.103
2.865ValTyr: 2.865 ± 0.214
0.0ValXaa: 0.0 ± 0.0
Trp
1.119TrpAla: 1.119 ± 0.109
0.147TrpCys: 0.147 ± 0.045
0.946TrpAsp: 0.946 ± 0.115
0.919TrpGlu: 0.919 ± 0.107
0.653TrpPhe: 0.653 ± 0.091
0.693TrpGly: 0.693 ± 0.103
0.373TrpHis: 0.373 ± 0.079
0.759TrpIle: 0.759 ± 0.118
0.919TrpLys: 0.919 ± 0.107
1.612TrpLeu: 1.612 ± 0.16
0.453TrpMet: 0.453 ± 0.07
0.6TrpAsn: 0.6 ± 0.087
0.533TrpPro: 0.533 ± 0.1
0.52TrpGln: 0.52 ± 0.078
0.773TrpArg: 0.773 ± 0.101
0.813TrpSer: 0.813 ± 0.101
0.959TrpThr: 0.959 ± 0.101
1.026TrpVal: 1.026 ± 0.119
0.306TrpTrp: 0.306 ± 0.082
0.72TrpTyr: 0.72 ± 0.108
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.691TyrAla: 2.691 ± 0.199
0.48TyrCys: 0.48 ± 0.089
2.878TyrAsp: 2.878 ± 0.193
2.025TyrGlu: 2.025 ± 0.152
1.412TyrPhe: 1.412 ± 0.125
3.078TyrGly: 3.078 ± 0.2
1.093TyrHis: 1.093 ± 0.129
2.119TyrIle: 2.119 ± 0.195
1.852TyrLys: 1.852 ± 0.162
3.531TyrLeu: 3.531 ± 0.218
1.186TyrMet: 1.186 ± 0.137
2.385TyrAsn: 2.385 ± 0.193
1.932TyrPro: 1.932 ± 0.142
1.879TyrGln: 1.879 ± 0.171
2.465TyrArg: 2.465 ± 0.195
2.385TyrSer: 2.385 ± 0.195
2.851TyrThr: 2.851 ± 0.197
2.518TyrVal: 2.518 ± 0.202
0.546TyrTrp: 0.546 ± 0.079
2.039TyrTyr: 2.039 ± 0.16
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 264 proteins (75053 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski