Oscillatoriales cyanobacterium USR001

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales; unclassified Oscillatoriales

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4908 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C0VT21|A0A1C0VT21_9CYAN DUF2281 domain-containing protein OS=Oscillatoriales cyanobacterium USR001 OX=1880991 GN=BCD67_02985 PE=4 SV=1
MM1 pKa = 7.72SGGLFGWIGDD11 pKa = 4.02GLAEE15 pKa = 4.32IASEE19 pKa = 4.27VIGVFKK25 pKa = 10.95SDD27 pKa = 4.26DD28 pKa = 3.63ASGSNPGATQGTTTSRR44 pKa = 11.84NQEE47 pKa = 3.92RR48 pKa = 11.84GRR50 pKa = 11.84ASYY53 pKa = 10.54LQGQDD58 pKa = 3.1LEE60 pKa = 5.4RR61 pKa = 11.84YY62 pKa = 7.93TEE64 pKa = 3.88QQLDD68 pKa = 3.92EE69 pKa = 5.07FGLNALWDD77 pKa = 4.16KK78 pKa = 11.62NNLVYY83 pKa = 10.86DD84 pKa = 4.17EE85 pKa = 4.76TLEE88 pKa = 4.15QYY90 pKa = 11.37GKK92 pKa = 6.96TTQVDD97 pKa = 3.52VLIRR101 pKa = 11.84DD102 pKa = 3.84GNDD105 pKa = 2.91NEE107 pKa = 4.38VAIIEE112 pKa = 4.51CKK114 pKa = 10.47AYY116 pKa = 8.78TSKK119 pKa = 10.73WNDD122 pKa = 3.27DD123 pKa = 3.82DD124 pKa = 6.13AVDD127 pKa = 3.45QARR130 pKa = 11.84RR131 pKa = 11.84LVSLANEE138 pKa = 4.04RR139 pKa = 11.84GIPLIFSTYY148 pKa = 10.93DD149 pKa = 2.93GTTDD153 pKa = 3.48CFGSRR158 pKa = 11.84VKK160 pKa = 10.58EE161 pKa = 4.02VLNQTQYY168 pKa = 11.4FVISSDD174 pKa = 3.66NIFFADD180 pKa = 3.77PSDD183 pKa = 3.64VKK185 pKa = 11.25EE186 pKa = 4.24NGVTQGLPPEE196 pKa = 4.93LRR198 pKa = 11.84SHH200 pKa = 6.36WDD202 pKa = 2.21WWFGQNYY209 pKa = 7.84EE210 pKa = 4.58PKK212 pKa = 9.85SSCRR216 pKa = 11.84SDD218 pKa = 3.64YY219 pKa = 11.4SSNDD223 pKa = 3.03PNGFDD228 pKa = 3.37SSYY231 pKa = 8.76EE232 pKa = 4.23TEE234 pKa = 4.47SSSSPDD240 pKa = 3.38YY241 pKa = 10.79EE242 pKa = 4.84RR243 pKa = 11.84SDD245 pKa = 3.76SADD248 pKa = 3.36SGGSWDD254 pKa = 5.58LFSNWFGDD262 pKa = 3.48SSEE265 pKa = 4.89AEE267 pKa = 3.98SSNNSNSSSINSRR280 pKa = 11.84DD281 pKa = 3.46SGNSWFSNWFDD292 pKa = 3.98DD293 pKa = 4.08SSEE296 pKa = 4.43SEE298 pKa = 4.22SSNASDD304 pKa = 3.77SSSINSNSRR313 pKa = 11.84DD314 pKa = 3.41SSDD317 pKa = 3.84YY318 pKa = 10.9GSSDD322 pKa = 3.69YY323 pKa = 11.64GSGDD327 pKa = 3.43YY328 pKa = 11.3GNSGDD333 pKa = 3.78SDD335 pKa = 4.29YY336 pKa = 11.71GSSHH340 pKa = 6.43YY341 pKa = 11.04GSGDD345 pKa = 3.39YY346 pKa = 11.0GDD348 pKa = 5.4SGDD351 pKa = 4.22SDD353 pKa = 4.2SGSSSSDD360 pKa = 3.19SGDD363 pKa = 3.21SDD365 pKa = 4.2YY366 pKa = 11.74GSSDD370 pKa = 3.75YY371 pKa = 11.61GSGDD375 pKa = 3.59YY376 pKa = 11.04GDD378 pKa = 4.93SSGSDD383 pKa = 3.36SSSSSSDD390 pKa = 3.22SWWW393 pKa = 3.06

Molecular weight: 42.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C0VW16|A0A1C0VW16_9CYAN Cytosine-specific methyltransferase OS=Oscillatoriales cyanobacterium USR001 OX=1880991 GN=BCD67_05635 PE=3 SV=1
MM1 pKa = 6.86TQQTLHH7 pKa = 5.03GTSRR11 pKa = 11.84KK12 pKa = 9.46RR13 pKa = 11.84KK14 pKa = 7.05RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TKK26 pKa = 10.26SGQAVISARR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05KK38 pKa = 10.18GRR40 pKa = 11.84HH41 pKa = 4.82RR42 pKa = 11.84LAVV45 pKa = 3.37

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4908

0

4908

1600603

31

3712

326.1

36.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.839 ± 0.037

0.969 ± 0.013

4.959 ± 0.027

6.709 ± 0.04

3.969 ± 0.022

6.672 ± 0.049

1.609 ± 0.018

7.32 ± 0.033

5.207 ± 0.036

10.888 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.884 ± 0.016

4.768 ± 0.035

4.646 ± 0.028

5.015 ± 0.035

5.006 ± 0.031

6.435 ± 0.036

5.543 ± 0.039

6.206 ± 0.029

1.366 ± 0.017

2.992 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski