Pasteurella phage PMP-GADVASU-IND

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L6BZJ4|A0A1L6BZJ4_9CAUD Uncharacterized protein OS=Pasteurella phage PMP-GADVASU-IND OX=1927015 PE=4 SV=1
MM1 pKa = 7.87IKK3 pKa = 10.32RR4 pKa = 11.84LLPIAAAALLLAACGAPEE22 pKa = 4.18STPAPEE28 pKa = 4.33STSVTTSASSTSSTTSTTSSAAPTTSAEE56 pKa = 4.06PTPTIEE62 pKa = 4.62PAPEE66 pKa = 3.95QPSPDD71 pKa = 3.42SEE73 pKa = 4.55YY74 pKa = 10.15PAQSTMIQHH83 pKa = 6.85GANAEE88 pKa = 4.18RR89 pKa = 11.84INVFEE94 pKa = 4.28DD95 pKa = 3.36TAGQFWLCDD104 pKa = 3.04KK105 pKa = 9.26MYY107 pKa = 10.54PNGSPEE113 pKa = 4.21SDD115 pKa = 4.2FYY117 pKa = 11.66AAPNGCAGPYY127 pKa = 9.94GSVNAAQEE135 pKa = 4.08GLARR139 pKa = 11.84AMHH142 pKa = 6.81DD143 pKa = 3.33ALAEE147 pKa = 4.04EE148 pKa = 5.03FDD150 pKa = 3.69IDD152 pKa = 5.76SNFDD156 pKa = 3.65DD157 pKa = 4.8YY158 pKa = 11.39ATPAAPEE165 pKa = 4.15PQSSDD170 pKa = 3.52EE171 pKa = 4.1IAQGIADD178 pKa = 3.95SMGCEE183 pKa = 3.13SAVFNEE189 pKa = 4.8EE190 pKa = 3.77IQGYY194 pKa = 9.75EE195 pKa = 4.19YY196 pKa = 11.31VDD198 pKa = 3.85CQQ200 pKa = 3.8

Molecular weight: 20.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L6BZH4|A0A1L6BZH4_9CAUD Uncharacterized protein OS=Pasteurella phage PMP-GADVASU-IND OX=1927015 PE=4 SV=1
MM1 pKa = 7.75AKK3 pKa = 9.95CQCTYY8 pKa = 10.88SYY10 pKa = 10.69PEE12 pKa = 3.69EE13 pKa = 4.14WLPVPGYY20 pKa = 9.74EE21 pKa = 4.83GLYY24 pKa = 9.69EE25 pKa = 4.11VSNHH29 pKa = 4.68GQVWSCGRR37 pKa = 11.84KK38 pKa = 8.83VLQKK42 pKa = 10.6NGRR45 pKa = 11.84IYY47 pKa = 10.61NRR49 pKa = 11.84KK50 pKa = 9.11PGMLKK55 pKa = 10.2ARR57 pKa = 11.84AGQKK61 pKa = 9.1YY62 pKa = 10.14ASVMLYY68 pKa = 10.98NNDD71 pKa = 2.94GVTKK75 pKa = 8.8TFRR78 pKa = 11.84IHH80 pKa = 6.65RR81 pKa = 11.84LVVTAFLGVIPDD93 pKa = 4.1DD94 pKa = 3.94LEE96 pKa = 3.97VRR98 pKa = 11.84HH99 pKa = 6.46WDD101 pKa = 3.37DD102 pKa = 3.71QPRR105 pKa = 11.84NNHH108 pKa = 6.15LSNLLLGDD116 pKa = 3.9RR117 pKa = 11.84SDD119 pKa = 3.61NMRR122 pKa = 11.84DD123 pKa = 3.17AVRR126 pKa = 11.84NGLHH130 pKa = 6.69PEE132 pKa = 3.82ASKK135 pKa = 8.94TVCVNGHH142 pKa = 5.43EE143 pKa = 4.48FTRR146 pKa = 11.84EE147 pKa = 3.54NTILIDD153 pKa = 3.68KK154 pKa = 10.05PNGRR158 pKa = 11.84SRR160 pKa = 11.84RR161 pKa = 11.84YY162 pKa = 9.26CRR164 pKa = 11.84KK165 pKa = 8.41CTQDD169 pKa = 2.78SRR171 pKa = 11.84AKK173 pKa = 9.69IHH175 pKa = 6.83RR176 pKa = 11.84KK177 pKa = 7.85SQEE180 pKa = 3.96KK181 pKa = 10.26VDD183 pKa = 3.39AGYY186 pKa = 10.05RR187 pKa = 11.84APKK190 pKa = 10.18RR191 pKa = 11.84FGGTCHH197 pKa = 7.05KK198 pKa = 10.02GHH200 pKa = 7.4PFGEE204 pKa = 4.48EE205 pKa = 3.09NTYY208 pKa = 10.57FYY210 pKa = 11.61ANGNRR215 pKa = 11.84EE216 pKa = 4.43CKK218 pKa = 7.57TCRR221 pKa = 11.84RR222 pKa = 11.84VRR224 pKa = 11.84DD225 pKa = 3.69KK226 pKa = 10.8IRR228 pKa = 11.84RR229 pKa = 11.84RR230 pKa = 11.84KK231 pKa = 9.2

Molecular weight: 26.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

13861

34

1569

213.2

23.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.771 ± 0.629

0.75 ± 0.191

6.594 ± 0.29

6.839 ± 0.252

3.059 ± 0.211

7.691 ± 0.523

2.186 ± 0.238

4.942 ± 0.176

5.057 ± 0.253

7.727 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.287 ± 0.12

3.816 ± 0.19

4.826 ± 0.305

3.694 ± 0.179

5.981 ± 0.44

6.709 ± 0.291

5.88 ± 0.273

6.695 ± 0.315

1.861 ± 0.144

2.633 ± 0.247
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski