Phytophthora cactorum

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae; Phytophthora

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23398 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A329RW33|A0A329RW33_9STRA Uncharacterized protein OS=Phytophthora cactorum OX=29920 GN=PC110_g14849 PE=4 SV=1
MM1 pKa = 7.77KK2 pKa = 10.45SPSSLVLLATAATFAFADD20 pKa = 3.49AGGIAAEE27 pKa = 4.45YY28 pKa = 8.03FTGDD32 pKa = 2.89GTAYY36 pKa = 9.79TLGDD40 pKa = 3.74TSSGNCNMMSALNFATTDD58 pKa = 3.58YY59 pKa = 11.49AALNNDD65 pKa = 3.04QWSGLQNCGRR75 pKa = 11.84CAEE78 pKa = 4.27VSCADD83 pKa = 4.19SRR85 pKa = 11.84CSDD88 pKa = 3.22QTKK91 pKa = 10.46SVVVQILDD99 pKa = 3.38RR100 pKa = 11.84CPEE103 pKa = 4.23CKK105 pKa = 10.06QGDD108 pKa = 4.41LDD110 pKa = 4.8LSPSVFKK117 pKa = 10.34TLTGSHH123 pKa = 7.3PSRR126 pKa = 11.84YY127 pKa = 7.04TIKK130 pKa = 10.03WKK132 pKa = 10.51FVDD135 pKa = 4.75CPVAGNVNYY144 pKa = 10.11CLKK147 pKa = 10.76GGSNNYY153 pKa = 5.91WTAIQPTNVATGVKK167 pKa = 9.46SLKK170 pKa = 10.43INGQATKK177 pKa = 9.46MLDD180 pKa = 2.98GAYY183 pKa = 10.49YY184 pKa = 10.59YY185 pKa = 11.17LLDD188 pKa = 4.53GAGTSQTDD196 pKa = 3.67LSSVSVSLTDD206 pKa = 3.33INGVSITDD214 pKa = 3.84KK215 pKa = 10.81VSLSAGSCAEE225 pKa = 4.29GSHH228 pKa = 5.46QFPSGGSPSKK238 pKa = 10.62PSTNTSTPTGAPKK251 pKa = 9.11PTTATPTMAPTKK263 pKa = 10.48VPTVTTKK270 pKa = 10.14ATTPTTPTPSQEE282 pKa = 4.25QQTPAPVPTTSTPMTDD298 pKa = 3.41TPASTTPTSTTQSGSGDD315 pKa = 3.85DD316 pKa = 3.98ASQTAQQDD324 pKa = 3.46LGSASRR330 pKa = 11.84NDD332 pKa = 4.08DD333 pKa = 3.41ATQSSPQASSSGNGDD348 pKa = 3.32SPEE351 pKa = 3.85QSIEE355 pKa = 3.79QDD357 pKa = 3.23PGSEE361 pKa = 4.23SNDD364 pKa = 3.18VEE366 pKa = 4.35TQAPITQQSSSSSEE380 pKa = 3.74GDD382 pKa = 3.06EE383 pKa = 4.33TQQQSSTDD391 pKa = 3.7GEE393 pKa = 4.72TSTEE397 pKa = 4.21SPDD400 pKa = 3.45VPATVAPSTPFSSASQQSGSGVDD423 pKa = 3.79SEE425 pKa = 5.17TEE427 pKa = 3.9ASSSASTYY435 pKa = 8.96MDD437 pKa = 4.46CGSSSHH443 pKa = 6.99PSEE446 pKa = 4.09QTPSSSNNDD455 pKa = 3.21NPSQEE460 pKa = 4.36SQQSAEE466 pKa = 3.82QDD468 pKa = 3.68LNSASVDD475 pKa = 3.43GNTDD479 pKa = 2.61NSTPIQQSGSTSDD492 pKa = 3.48NDD494 pKa = 3.7GTQVTQQDD502 pKa = 4.07PDD504 pKa = 3.92SVDD507 pKa = 4.21SEE509 pKa = 4.68DD510 pKa = 3.63STVDD514 pKa = 3.34NASSAVGADD523 pKa = 2.84VDD525 pKa = 4.22MVISQSGSADD535 pKa = 3.46QNDD538 pKa = 4.34EE539 pKa = 4.28NPVQQTSSDD548 pKa = 3.5VGADD552 pKa = 3.51TDD554 pKa = 4.63SEE556 pKa = 4.72TPEE559 pKa = 3.82QTSNEE564 pKa = 4.21DD565 pKa = 3.81GEE567 pKa = 4.93TEE569 pKa = 4.26TPQQDD574 pKa = 3.22SSSANTEE581 pKa = 3.85TTEE584 pKa = 4.24IPSSTGTANCPMM596 pKa = 5.11

Molecular weight:
61.03 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A329S3I7|A0A329S3I7_9STRA Dymeclin OS=Phytophthora cactorum OX=29920 GN=PC110_g12338 PE=3 SV=1
MM1 pKa = 7.31KK2 pKa = 10.19RR3 pKa = 11.84WILPTLKK10 pKa = 10.29LARR13 pKa = 11.84VRR15 pKa = 11.84TLPVVLNKK23 pKa = 9.78IKK25 pKa = 9.81KK26 pKa = 8.41TITSFKK32 pKa = 10.65KK33 pKa = 10.45SLQQLSTNSPQRR45 pKa = 11.84MFRR48 pKa = 11.84SSLKK52 pKa = 10.44KK53 pKa = 10.38

Molecular weight:
6.24 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23095

303

23398

9185206

24

10474

392.6

43.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.859 ± 0.015

1.621 ± 0.008

5.698 ± 0.011

6.655 ± 0.023

3.711 ± 0.011

6.046 ± 0.019

2.315 ± 0.008

4.14 ± 0.012

5.168 ± 0.017

9.335 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.477 ± 0.008

3.582 ± 0.009

4.7 ± 0.014

4.226 ± 0.014

6.22 ± 0.02

8.289 ± 0.021

6.018 ± 0.018

7.121 ± 0.016

1.209 ± 0.005

2.604 ± 0.009

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski