Pararhizobium sp. BGMRC6574

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Pararhizobium

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3546 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A506U7A4|A0A506U7A4_9RHIZ Glucose-6-phosphate 1-dehydrogenase OS=Pararhizobium sp. BGMRC6574 OX=2590452 GN=zwf PE=3 SV=1
MM1 pKa = 7.21MKK3 pKa = 10.54KK4 pKa = 10.28LLTGAALAVVLSTPAFAADD23 pKa = 3.39MVMSPEE29 pKa = 4.13PAPAPVQTAPSFSWTGFYY47 pKa = 10.82VGGQLGYY54 pKa = 11.27AFTDD58 pKa = 3.61VSGDD62 pKa = 3.68GADD65 pKa = 4.37DD66 pKa = 4.78DD67 pKa = 5.8DD68 pKa = 5.94GISGGGMVGYY78 pKa = 10.49NYY80 pKa = 10.83DD81 pKa = 3.27LGGFVAGAEE90 pKa = 4.01VGYY93 pKa = 10.81DD94 pKa = 3.39FMGSDD99 pKa = 4.28FGNGVGADD107 pKa = 3.05GVMQAKK113 pKa = 9.98LRR115 pKa = 11.84VGADD119 pKa = 3.41LGRR122 pKa = 11.84TLLYY126 pKa = 9.59GTGGYY131 pKa = 10.91ALLHH135 pKa = 6.63GDD137 pKa = 3.4ASEE140 pKa = 4.66DD141 pKa = 3.17KK142 pKa = 10.21SGYY145 pKa = 10.09VIGAGVDD152 pKa = 3.56YY153 pKa = 10.77AISDD157 pKa = 3.69NTFVGAEE164 pKa = 3.78YY165 pKa = 10.23DD166 pKa = 3.85YY167 pKa = 11.89NSFDD171 pKa = 3.91NVEE174 pKa = 3.89SHH176 pKa = 7.03ANNNEE181 pKa = 3.72SDD183 pKa = 3.63VDD185 pKa = 3.95FHH187 pKa = 6.49QVKK190 pKa = 10.35ARR192 pKa = 11.84VGFKK196 pKa = 10.13FF197 pKa = 3.44

Molecular weight: 20.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A506U673|A0A506U673_9RHIZ 50S ribosomal protein L24 OS=Pararhizobium sp. BGMRC6574 OX=2590452 GN=rplX PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.84LVRR13 pKa = 11.84KK14 pKa = 9.15RR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 4.55GFRR20 pKa = 11.84ARR22 pKa = 11.84LATKK26 pKa = 10.16GGRR29 pKa = 11.84KK30 pKa = 9.14VLSARR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84KK41 pKa = 9.03RR42 pKa = 11.84LSAA45 pKa = 4.03

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3546

0

3546

1155526

28

2838

325.9

35.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.368 ± 0.053

0.848 ± 0.011

6.094 ± 0.034

6.113 ± 0.04

3.799 ± 0.027

8.624 ± 0.034

2.126 ± 0.019

5.0 ± 0.032

3.165 ± 0.032

9.566 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.555 ± 0.017

2.56 ± 0.024

4.957 ± 0.029

2.715 ± 0.02

7.565 ± 0.049

5.496 ± 0.032

5.476 ± 0.02

7.538 ± 0.032

1.229 ± 0.016

2.206 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski