Janthinobacterium sp. KBS0711 
Average proteome isoelectric point is 6.75 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 5384 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A0M2WRK1|A0A0M2WRK1_9BURK Hca operon transcriptional activator OS=Janthinobacterium sp. KBS0711 OX=1649647 GN=hcaR_2 PE=3 SV=1MM1 pKa = 7.1  NAVAEE6 pKa = 4.3  MQDD9 pKa = 4.15  VIPSPIIFTDD19 pKa = 3.6  SAAEE23 pKa = 4.01  KK24 pKa = 9.59  VAQLIEE30 pKa = 4.38  EE31 pKa = 4.51  EE32 pKa = 4.63  GNPDD36 pKa = 2.85  LKK38 pKa = 11.13  LRR40 pKa = 11.84  VFVQGGGCSGFQYY53 pKa = 10.94  GFTFDD58 pKa = 4.69  EE59 pKa = 4.93  IVNEE63 pKa = 4.83  DD64 pKa = 3.85  DD65 pKa = 3.11  TTMVKK70 pKa = 10.58  NGVQLLIDD78 pKa = 3.77  SMSYY82 pKa = 10.23  QYY84 pKa = 11.55  LVGAEE89 pKa = 4.01  IDD91 pKa = 3.89  YY92 pKa = 11.28  KK93 pKa = 11.39  DD94 pKa = 4.47  DD95 pKa = 4.08  LEE97 pKa = 4.56  GAQFVIKK104 pKa = 10.68  NPTATSTCGCGSSFSVV120 pKa = 3.54  
 12.98 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.752 
IPC2_protein 3.834 
IPC_protein 3.77 
Toseland    3.567 
ProMoST     3.935 
Dawson      3.757 
Bjellqvist  3.923 
Wikipedia   3.694 
Rodwell     3.605 
Grimsley    3.49 
Solomon     3.745 
Lehninger   3.694 
Nozaki      3.884 
DTASelect   4.075 
Thurlkill   3.63 
EMBOSS      3.706 
Sillero     3.897 
Patrickios  1.875 
IPC_peptide 3.745 
IPC2_peptide  3.872 
IPC2.peptide.svr19  3.808 
 Protein with the highest isoelectric point: 
>tr|A0A0M2WPU7|A0A0M2WPU7_9BURK Inosine 5'-monophosphate dehydrogenase OS=Janthinobacterium sp. KBS0711 OX=1649647 GN=VM94_01574 PE=4 SV=1MM1 pKa = 7.91  PKK3 pKa = 9.63  MKK5 pKa = 9.83  TKK7 pKa = 10.72  SSAKK11 pKa = 9.15  KK12 pKa = 9.67  RR13 pKa = 11.84  FRR15 pKa = 11.84  VRR17 pKa = 11.84  PGGTVKK23 pKa = 10.48  SGHH26 pKa = 5.47  AFKK29 pKa = 10.85  RR30 pKa = 11.84  HH31 pKa = 5.44  ILTKK35 pKa = 9.78  KK36 pKa = 3.98  TTKK39 pKa = 10.23  NKK41 pKa = 9.21  RR42 pKa = 11.84  QLRR45 pKa = 11.84  GTRR48 pKa = 11.84  NINASDD54 pKa = 3.65  VTSVMRR60 pKa = 11.84  MMPTAA65 pKa = 4.09  
 7.38 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.358 
IPC2_protein 10.789 
IPC_protein 12.325 
Toseland    12.501 
ProMoST     12.983 
Dawson      12.515 
Bjellqvist  12.486 
Wikipedia   12.969 
Rodwell     12.427 
Grimsley    12.544 
Solomon     12.983 
Lehninger   12.896 
Nozaki      12.501 
DTASelect   12.486 
Thurlkill   12.501 
EMBOSS      12.998 
Sillero     12.501 
Patrickios  12.149 
IPC_peptide 12.998 
IPC2_peptide  11.974 
IPC2.peptide.svr19  9.0 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        5384 
0
5384 
1820683
29
4558
338.2
36.67
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        12.81 ± 0.048
0.9 ± 0.01
5.278 ± 0.023
4.897 ± 0.031
3.549 ± 0.021
8.09 ± 0.037
2.218 ± 0.016
4.764 ± 0.027
3.629 ± 0.03
10.912 ± 0.044
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.574 ± 0.017
2.976 ± 0.021
4.906 ± 0.027
4.334 ± 0.023
6.124 ± 0.034
5.693 ± 0.029
5.207 ± 0.027
7.197 ± 0.027
1.341 ± 0.014
2.566 ± 0.02
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here