Demequina mangrovi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Demequinaceae; Demequina

Average proteome isoelectric point is 5.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2745 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H6XH86|A0A1H6XH86_9MICO Ribonucleoside-diphosphate reductase OS=Demequina mangrovi OX=1043493 GN=SAMN05421637_1293 PE=3 SV=1
MM1 pKa = 6.82QRR3 pKa = 11.84RR4 pKa = 11.84TIVPPLVLALAATAVVSAPASAAAPDD30 pKa = 4.15LFISEE35 pKa = 4.53YY36 pKa = 11.08VEE38 pKa = 4.04GSGFNKK44 pKa = 10.02AVEE47 pKa = 4.31IFNGTGAAVDD57 pKa = 3.88LADD60 pKa = 3.86YY61 pKa = 11.14AVVPYY66 pKa = 10.47HH67 pKa = 6.6NGSTDD72 pKa = 3.28PYY74 pKa = 8.47TTIEE78 pKa = 3.95LSGTLEE84 pKa = 4.34PGDD87 pKa = 3.73VFVVASSTTSSTNDD101 pKa = 2.81VAALADD107 pKa = 3.93LTTGSVAWNGNDD119 pKa = 4.09AVLLTHH125 pKa = 7.46DD126 pKa = 4.28GATIDD131 pKa = 3.49VLGTVGDD138 pKa = 4.02DD139 pKa = 4.19SNWGANVTLRR149 pKa = 11.84RR150 pKa = 11.84LEE152 pKa = 4.43SICAGAAAYY161 pKa = 10.11SAGDD165 pKa = 3.42WDD167 pKa = 4.7SYY169 pKa = 11.79ASNTVDD175 pKa = 3.91GLGTHH180 pKa = 6.56TASCDD185 pKa = 3.19GGPVDD190 pKa = 5.56DD191 pKa = 5.49AAPALASSSPEE202 pKa = 4.02DD203 pKa = 3.43GATGVNAAAEE213 pKa = 4.21LTVTFTEE220 pKa = 4.49PVAAEE225 pKa = 3.94GAFALVCDD233 pKa = 4.81DD234 pKa = 3.84ATVPAAVTGGPTTWTVAPEE253 pKa = 4.03TEE255 pKa = 4.09LAAGAVCVLTVTAAAVTDD273 pKa = 4.47LDD275 pKa = 4.01EE276 pKa = 4.76TDD278 pKa = 4.53PPDD281 pKa = 4.43AMEE284 pKa = 4.92ADD286 pKa = 3.52AVIGFTVADD295 pKa = 4.82GPTLIHH301 pKa = 7.11DD302 pKa = 4.01VQGTEE307 pKa = 3.88DD308 pKa = 3.26TAAMVGEE315 pKa = 4.4TVTIEE320 pKa = 4.04GVVVGDD326 pKa = 3.95YY327 pKa = 9.85EE328 pKa = 4.94GPSPTLRR335 pKa = 11.84GFYY338 pKa = 10.03VQEE341 pKa = 3.9EE342 pKa = 4.34DD343 pKa = 4.32ADD345 pKa = 4.08ADD347 pKa = 4.04ADD349 pKa = 4.07PSTSEE354 pKa = 4.83AIFVYY359 pKa = 10.02HH360 pKa = 6.8GSSDD364 pKa = 3.57TVEE367 pKa = 4.29VGDD370 pKa = 4.16LVSVTGTVAEE380 pKa = 4.6RR381 pKa = 11.84FGQTQLGYY389 pKa = 9.85PSALTVLDD397 pKa = 3.63SGLTVTPAAVSLPFADD413 pKa = 3.48ADD415 pKa = 3.54EE416 pKa = 4.67AEE418 pKa = 4.81RR419 pKa = 11.84YY420 pKa = 10.02EE421 pKa = 4.18GMLVEE426 pKa = 5.01FAQTLTVTEE435 pKa = 4.76LYY437 pKa = 10.42EE438 pKa = 3.92LGRR441 pKa = 11.84FGEE444 pKa = 4.65VTVSSGGRR452 pKa = 11.84LDD454 pKa = 3.53QPTAVVEE461 pKa = 4.48PGADD465 pKa = 3.43AIALQEE471 pKa = 4.21ANNLRR476 pKa = 11.84TVIVDD481 pKa = 3.81DD482 pKa = 4.01ATNAQNPDD490 pKa = 3.09VLMGRR495 pKa = 11.84GGDD498 pKa = 3.67PLSAEE503 pKa = 3.86NTLRR507 pKa = 11.84GGDD510 pKa = 4.05TITGLTGVMTFGYY523 pKa = 10.17DD524 pKa = 3.26GYY526 pKa = 11.07YY527 pKa = 10.25SSSNAWRR534 pKa = 11.84VRR536 pKa = 11.84PVDD539 pKa = 3.57ALPAFEE545 pKa = 5.13AVNARR550 pKa = 11.84PTEE553 pKa = 4.06VPDD556 pKa = 3.22VGGDD560 pKa = 3.5VQVASFNVLNYY571 pKa = 10.19FLTLDD576 pKa = 4.69AGDD579 pKa = 4.09NQCGPVGSEE588 pKa = 3.77NDD590 pKa = 4.57CRR592 pKa = 11.84GAWNATEE599 pKa = 5.8LEE601 pKa = 4.16RR602 pKa = 11.84QTDD605 pKa = 3.69KK606 pKa = 11.35LVAALAGLDD615 pKa = 3.47ADD617 pKa = 4.46VIGIMEE623 pKa = 4.45MEE625 pKa = 4.32NTTGVEE631 pKa = 3.77PLAYY635 pKa = 10.21LAEE638 pKa = 4.04QLNAYY643 pKa = 9.89LGTDD647 pKa = 2.73TWSYY651 pKa = 11.26VDD653 pKa = 3.75TGTIGTDD660 pKa = 3.51VIRR663 pKa = 11.84VGILYY668 pKa = 10.39DD669 pKa = 3.49SAVVSEE675 pKa = 4.84LGDD678 pKa = 3.65FAVLDD683 pKa = 3.9STVDD687 pKa = 3.15ARR689 pKa = 11.84FDD691 pKa = 3.46DD692 pKa = 3.91TRR694 pKa = 11.84NRR696 pKa = 11.84PSLAQTFVTEE706 pKa = 4.23DD707 pKa = 3.33DD708 pKa = 3.74AAFTVVTNHH717 pKa = 6.36LKK719 pKa = 10.81SKK721 pKa = 10.24GCSEE725 pKa = 4.52SSGADD730 pKa = 3.16ADD732 pKa = 3.86QGDD735 pKa = 4.95GAGCWNEE742 pKa = 3.78SRR744 pKa = 11.84TLAAEE749 pKa = 4.25ALVDD753 pKa = 3.73WLASDD758 pKa = 3.79PTDD761 pKa = 3.9AHH763 pKa = 6.92DD764 pKa = 5.32ADD766 pKa = 4.24ALVIGDD772 pKa = 3.96LNSYY776 pKa = 10.21GMEE779 pKa = 4.24DD780 pKa = 4.06PIDD783 pKa = 3.7VLRR786 pKa = 11.84EE787 pKa = 3.75AGYY790 pKa = 10.94VEE792 pKa = 5.47LGGGDD797 pKa = 3.63YY798 pKa = 10.97SYY800 pKa = 11.59VFSGQWGSLDD810 pKa = 3.45YY811 pKa = 11.07TFASPSLAAQVTGSAHH827 pKa = 3.8VHH829 pKa = 5.76INADD833 pKa = 3.54EE834 pKa = 4.09PSILDD839 pKa = 3.11YY840 pKa = 7.11TTRR843 pKa = 11.84YY844 pKa = 9.23KK845 pKa = 10.71PSALVEE851 pKa = 4.17SLYY854 pKa = 11.25APDD857 pKa = 3.52WYY859 pKa = 9.59RR860 pKa = 11.84TSDD863 pKa = 3.54HH864 pKa = 7.16DD865 pKa = 4.35PVVLGLDD872 pKa = 3.7LDD874 pKa = 4.31TPVCEE879 pKa = 3.93VSYY882 pKa = 10.71AVNGTWPGGFISQLWIRR899 pKa = 11.84NVSDD903 pKa = 3.65EE904 pKa = 5.54PIDD907 pKa = 3.7GWQLGWDD914 pKa = 3.66FAGDD918 pKa = 4.01EE919 pKa = 4.66EE920 pKa = 5.7VSSLWGGTVAQDD932 pKa = 3.57GAAVTVDD939 pKa = 3.36AMPWNSVLAPVSEE952 pKa = 4.38VKK954 pKa = 10.39GKK956 pKa = 10.39KK957 pKa = 9.57KK958 pKa = 10.89SLGTTLGFLGTSQAGPEE975 pKa = 4.17DD976 pKa = 3.68VTAFTLNGEE985 pKa = 4.3RR986 pKa = 11.84CSVGG990 pKa = 3.09

Molecular weight: 102.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H6ZWS1|A0A1H6ZWS1_9MICO Chemotaxis protein MotA OS=Demequina mangrovi OX=1043493 GN=SAMN05421637_2194 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84KK12 pKa = 8.98RR13 pKa = 11.84AKK15 pKa = 8.46THH17 pKa = 5.11GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SIMASRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 10.21GRR40 pKa = 11.84GKK42 pKa = 10.78LSAA45 pKa = 4.32

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2745

0

2745

912256

29

4176

332.3

35.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.526 ± 0.08

0.599 ± 0.013

6.385 ± 0.054

6.292 ± 0.054

2.771 ± 0.03

9.097 ± 0.047

2.072 ± 0.024

4.094 ± 0.036

1.824 ± 0.035

9.855 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.033 ± 0.021

1.646 ± 0.028

5.27 ± 0.041

2.432 ± 0.023

7.174 ± 0.065

5.236 ± 0.034

5.993 ± 0.056

9.224 ± 0.043

1.525 ± 0.022

1.952 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski