Amino acid dipepetide frequency for Klebsiella phage phiBO1E

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.63AlaAla: 15.63 ± 1.338
0.514AlaCys: 0.514 ± 0.2
6.09AlaAsp: 6.09 ± 0.562
4.696AlaGlu: 4.696 ± 0.489
3.742AlaPhe: 3.742 ± 0.439
8.218AlaGly: 8.218 ± 1.058
1.027AlaHis: 1.027 ± 0.301
4.77AlaIle: 4.77 ± 0.674
5.357AlaLys: 5.357 ± 1.038
9.539AlaLeu: 9.539 ± 0.838
2.642AlaMet: 2.642 ± 0.352
2.935AlaAsn: 2.935 ± 0.308
4.183AlaPro: 4.183 ± 0.714
5.136AlaGln: 5.136 ± 0.759
6.164AlaArg: 6.164 ± 0.731
5.063AlaSer: 5.063 ± 0.643
5.136AlaThr: 5.136 ± 0.724
7.264AlaVal: 7.264 ± 0.884
1.174AlaTrp: 1.174 ± 0.287
4.476AlaTyr: 4.476 ± 0.541
0.0AlaXaa: 0.0 ± 0.0
Cys
0.881CysAla: 0.881 ± 0.288
0.44CysCys: 0.44 ± 0.265
0.44CysAsp: 0.44 ± 0.17
0.587CysGlu: 0.587 ± 0.232
0.44CysPhe: 0.44 ± 0.206
0.514CysGly: 0.514 ± 0.191
0.367CysHis: 0.367 ± 0.158
0.367CysIle: 0.367 ± 0.189
0.44CysLys: 0.44 ± 0.232
0.954CysLeu: 0.954 ± 0.268
0.587CysMet: 0.587 ± 0.211
0.294CysAsn: 0.294 ± 0.156
0.66CysPro: 0.66 ± 0.238
0.22CysGln: 0.22 ± 0.138
0.66CysArg: 0.66 ± 0.196
1.174CysSer: 1.174 ± 0.37
1.027CysThr: 1.027 ± 0.314
1.101CysVal: 1.101 ± 0.287
0.22CysTrp: 0.22 ± 0.122
0.44CysTyr: 0.44 ± 0.176
0.0CysXaa: 0.0 ± 0.0
Asp
7.558AspAla: 7.558 ± 0.93
0.954AspCys: 0.954 ± 0.328
3.522AspAsp: 3.522 ± 0.45
3.082AspGlu: 3.082 ± 0.452
2.568AspPhe: 2.568 ± 0.361
4.256AspGly: 4.256 ± 0.441
0.587AspHis: 0.587 ± 0.21
3.229AspIle: 3.229 ± 0.415
2.642AspLys: 2.642 ± 0.489
4.99AspLeu: 4.99 ± 0.563
2.642AspMet: 2.642 ± 0.407
2.935AspAsn: 2.935 ± 0.573
2.348AspPro: 2.348 ± 0.432
1.541AspGln: 1.541 ± 0.345
2.421AspArg: 2.421 ± 0.512
4.916AspSer: 4.916 ± 0.589
4.036AspThr: 4.036 ± 0.53
3.742AspVal: 3.742 ± 0.437
1.027AspTrp: 1.027 ± 0.2
2.568AspTyr: 2.568 ± 0.411
0.0AspXaa: 0.0 ± 0.0
Glu
6.017GluAla: 6.017 ± 0.73
0.44GluCys: 0.44 ± 0.181
2.642GluAsp: 2.642 ± 0.389
4.036GluGlu: 4.036 ± 0.924
2.348GluPhe: 2.348 ± 0.367
4.256GluGly: 4.256 ± 0.828
2.788GluHis: 2.788 ± 0.436
2.421GluIle: 2.421 ± 0.44
1.981GluLys: 1.981 ± 0.426
5.136GluLeu: 5.136 ± 0.611
2.275GluMet: 2.275 ± 0.393
1.761GluAsn: 1.761 ± 0.368
1.834GluPro: 1.834 ± 0.318
3.375GluGln: 3.375 ± 0.507
3.889GluArg: 3.889 ± 0.538
2.642GluSer: 2.642 ± 0.528
2.642GluThr: 2.642 ± 0.453
4.843GluVal: 4.843 ± 0.492
0.954GluTrp: 0.954 ± 0.228
2.421GluTyr: 2.421 ± 0.523
0.0GluXaa: 0.0 ± 0.0
Phe
2.862PheAla: 2.862 ± 0.419
0.514PheCys: 0.514 ± 0.193
2.128PheAsp: 2.128 ± 0.384
2.055PheGlu: 2.055 ± 0.408
1.394PhePhe: 1.394 ± 0.252
2.421PheGly: 2.421 ± 0.362
0.367PheHis: 0.367 ± 0.141
1.247PheIle: 1.247 ± 0.287
2.201PheLys: 2.201 ± 0.418
2.128PheLeu: 2.128 ± 0.382
0.66PheMet: 0.66 ± 0.268
1.688PheAsn: 1.688 ± 0.325
1.321PhePro: 1.321 ± 0.275
1.321PheGln: 1.321 ± 0.254
1.834PheArg: 1.834 ± 0.337
1.834PheSer: 1.834 ± 0.442
2.348PheThr: 2.348 ± 0.435
1.834PheVal: 1.834 ± 0.473
0.514PheTrp: 0.514 ± 0.172
1.688PheTyr: 1.688 ± 0.28
0.0PheXaa: 0.0 ± 0.0
Gly
5.944GlyAla: 5.944 ± 0.664
1.468GlyCys: 1.468 ± 0.345
4.623GlyAsp: 4.623 ± 0.636
4.109GlyGlu: 4.109 ± 0.449
2.715GlyPhe: 2.715 ± 0.496
4.843GlyGly: 4.843 ± 0.64
1.174GlyHis: 1.174 ± 0.282
4.256GlyIle: 4.256 ± 0.709
3.742GlyLys: 3.742 ± 0.68
6.531GlyLeu: 6.531 ± 0.608
1.981GlyMet: 1.981 ± 0.442
3.596GlyAsn: 3.596 ± 0.731
1.614GlyPro: 1.614 ± 0.323
3.009GlyGln: 3.009 ± 0.411
4.843GlyArg: 4.843 ± 0.52
5.357GlySer: 5.357 ± 0.62
4.696GlyThr: 4.696 ± 0.742
6.09GlyVal: 6.09 ± 0.746
0.587GlyTrp: 0.587 ± 0.201
3.155GlyTyr: 3.155 ± 0.562
0.0GlyXaa: 0.0 ± 0.0
His
1.321HisAla: 1.321 ± 0.344
0.367HisCys: 0.367 ± 0.16
0.954HisAsp: 0.954 ± 0.251
1.394HisGlu: 1.394 ± 0.364
0.367HisPhe: 0.367 ± 0.138
1.908HisGly: 1.908 ± 0.525
0.073HisHis: 0.073 ± 0.062
0.954HisIle: 0.954 ± 0.278
0.807HisLys: 0.807 ± 0.231
2.128HisLeu: 2.128 ± 0.433
0.44HisMet: 0.44 ± 0.15
0.881HisAsn: 0.881 ± 0.24
0.734HisPro: 0.734 ± 0.342
0.44HisGln: 0.44 ± 0.181
1.688HisArg: 1.688 ± 0.35
1.174HisSer: 1.174 ± 0.281
0.66HisThr: 0.66 ± 0.204
0.954HisVal: 0.954 ± 0.21
0.22HisTrp: 0.22 ± 0.108
0.954HisTyr: 0.954 ± 0.302
0.0HisXaa: 0.0 ± 0.0
Ile
3.522IleAla: 3.522 ± 0.644
0.367IleCys: 0.367 ± 0.173
3.229IleAsp: 3.229 ± 0.382
2.348IleGlu: 2.348 ± 0.498
0.587IlePhe: 0.587 ± 0.166
2.495IleGly: 2.495 ± 0.406
0.807IleHis: 0.807 ± 0.248
2.055IleIle: 2.055 ± 0.404
3.155IleLys: 3.155 ± 0.402
4.329IleLeu: 4.329 ± 0.561
1.101IleMet: 1.101 ± 0.215
2.128IleAsn: 2.128 ± 0.455
2.201IlePro: 2.201 ± 0.366
2.495IleGln: 2.495 ± 0.449
2.935IleArg: 2.935 ± 0.387
3.375IleSer: 3.375 ± 0.53
2.568IleThr: 2.568 ± 0.485
2.568IleVal: 2.568 ± 0.384
0.073IleTrp: 0.073 ± 0.072
0.954IleTyr: 0.954 ± 0.203
0.0IleXaa: 0.0 ± 0.0
Lys
5.944LysAla: 5.944 ± 0.781
0.514LysCys: 0.514 ± 0.196
2.788LysAsp: 2.788 ± 0.385
3.155LysGlu: 3.155 ± 0.523
1.247LysPhe: 1.247 ± 0.308
3.155LysGly: 3.155 ± 0.601
0.881LysHis: 0.881 ± 0.295
1.394LysIle: 1.394 ± 0.267
2.055LysLys: 2.055 ± 0.34
4.916LysLeu: 4.916 ± 0.674
1.394LysMet: 1.394 ± 0.287
1.468LysAsn: 1.468 ± 0.294
1.468LysPro: 1.468 ± 0.348
3.889LysGln: 3.889 ± 0.535
3.229LysArg: 3.229 ± 0.52
2.715LysSer: 2.715 ± 0.384
2.348LysThr: 2.348 ± 0.436
3.302LysVal: 3.302 ± 0.43
1.101LysTrp: 1.101 ± 0.231
1.688LysTyr: 1.688 ± 0.348
0.0LysXaa: 0.0 ± 0.0
Leu
8.659LeuAla: 8.659 ± 0.877
1.247LeuCys: 1.247 ± 0.342
7.118LeuAsp: 7.118 ± 0.694
5.724LeuGlu: 5.724 ± 0.582
2.495LeuPhe: 2.495 ± 0.392
6.677LeuGly: 6.677 ± 0.623
1.541LeuHis: 1.541 ± 0.35
3.962LeuIle: 3.962 ± 0.568
3.155LeuLys: 3.155 ± 0.45
6.677LeuLeu: 6.677 ± 0.66
1.908LeuMet: 1.908 ± 0.404
3.375LeuAsn: 3.375 ± 0.467
3.155LeuPro: 3.155 ± 0.448
4.476LeuGln: 4.476 ± 0.51
6.164LeuArg: 6.164 ± 0.619
4.99LeuSer: 4.99 ± 0.541
5.283LeuThr: 5.283 ± 0.585
6.237LeuVal: 6.237 ± 0.826
1.174LeuTrp: 1.174 ± 0.297
3.229LeuTyr: 3.229 ± 0.472
0.0LeuXaa: 0.0 ± 0.0
Met
3.229MetAla: 3.229 ± 0.546
0.294MetCys: 0.294 ± 0.147
2.055MetAsp: 2.055 ± 0.427
1.174MetGlu: 1.174 ± 0.262
1.027MetPhe: 1.027 ± 0.249
1.394MetGly: 1.394 ± 0.263
0.881MetHis: 0.881 ± 0.241
0.734MetIle: 0.734 ± 0.228
1.101MetLys: 1.101 ± 0.284
3.669MetLeu: 3.669 ± 0.498
0.734MetMet: 0.734 ± 0.252
0.881MetAsn: 0.881 ± 0.27
1.174MetPro: 1.174 ± 0.27
2.055MetGln: 2.055 ± 0.355
1.834MetArg: 1.834 ± 0.443
2.421MetSer: 2.421 ± 0.479
0.954MetThr: 0.954 ± 0.331
2.055MetVal: 2.055 ± 0.397
0.44MetTrp: 0.44 ± 0.175
1.174MetTyr: 1.174 ± 0.27
0.0MetXaa: 0.0 ± 0.0
Asn
3.155AsnAla: 3.155 ± 0.434
0.22AsnCys: 0.22 ± 0.107
1.981AsnAsp: 1.981 ± 0.391
1.614AsnGlu: 1.614 ± 0.292
0.954AsnPhe: 0.954 ± 0.228
3.082AsnGly: 3.082 ± 0.428
0.294AsnHis: 0.294 ± 0.136
2.128AsnIle: 2.128 ± 0.419
2.348AsnLys: 2.348 ± 0.331
2.862AsnLeu: 2.862 ± 0.526
1.101AsnMet: 1.101 ± 0.308
1.688AsnAsn: 1.688 ± 0.372
2.275AsnPro: 2.275 ± 0.366
1.761AsnGln: 1.761 ± 0.363
2.201AsnArg: 2.201 ± 0.39
2.788AsnSer: 2.788 ± 0.586
3.229AsnThr: 3.229 ± 0.534
3.742AsnVal: 3.742 ± 0.425
0.734AsnTrp: 0.734 ± 0.267
1.761AsnTyr: 1.761 ± 0.339
0.0AsnXaa: 0.0 ± 0.0
Pro
3.816ProAla: 3.816 ± 0.717
0.294ProCys: 0.294 ± 0.129
2.568ProAsp: 2.568 ± 0.441
3.669ProGlu: 3.669 ± 0.379
1.321ProPhe: 1.321 ± 0.238
2.862ProGly: 2.862 ± 0.618
0.44ProHis: 0.44 ± 0.178
1.834ProIle: 1.834 ± 0.356
1.394ProLys: 1.394 ± 0.344
2.715ProLeu: 2.715 ± 0.386
0.954ProMet: 0.954 ± 0.265
1.468ProAsn: 1.468 ± 0.335
1.101ProPro: 1.101 ± 0.325
1.468ProGln: 1.468 ± 0.317
1.908ProArg: 1.908 ± 0.335
2.568ProSer: 2.568 ± 0.441
2.275ProThr: 2.275 ± 0.416
3.082ProVal: 3.082 ± 0.488
0.587ProTrp: 0.587 ± 0.236
1.394ProTyr: 1.394 ± 0.365
0.0ProXaa: 0.0 ± 0.0
Gln
5.21GlnAla: 5.21 ± 0.717
0.367GlnCys: 0.367 ± 0.17
2.935GlnAsp: 2.935 ± 0.52
3.449GlnGlu: 3.449 ± 0.475
1.468GlnPhe: 1.468 ± 0.351
2.862GlnGly: 2.862 ± 0.453
1.541GlnHis: 1.541 ± 0.364
0.881GlnIle: 0.881 ± 0.273
2.568GlnLys: 2.568 ± 0.444
4.99GlnLeu: 4.99 ± 0.503
1.174GlnMet: 1.174 ± 0.236
2.055GlnAsn: 2.055 ± 0.379
1.688GlnPro: 1.688 ± 0.418
2.715GlnGln: 2.715 ± 0.518
2.862GlnArg: 2.862 ± 0.433
3.596GlnSer: 3.596 ± 0.491
1.688GlnThr: 1.688 ± 0.454
2.862GlnVal: 2.862 ± 0.39
0.66GlnTrp: 0.66 ± 0.214
2.055GlnTyr: 2.055 ± 0.486
0.0GlnXaa: 0.0 ± 0.0
Arg
6.384ArgAla: 6.384 ± 1.001
0.514ArgCys: 0.514 ± 0.222
3.449ArgAsp: 3.449 ± 0.523
3.962ArgGlu: 3.962 ± 0.526
2.275ArgPhe: 2.275 ± 0.382
4.476ArgGly: 4.476 ± 0.663
1.247ArgHis: 1.247 ± 0.277
3.155ArgIle: 3.155 ± 0.525
3.302ArgLys: 3.302 ± 0.541
4.916ArgLeu: 4.916 ± 0.516
2.055ArgMet: 2.055 ± 0.399
2.421ArgAsn: 2.421 ± 0.354
1.394ArgPro: 1.394 ± 0.352
2.862ArgGln: 2.862 ± 0.381
4.256ArgArg: 4.256 ± 0.666
2.421ArgSer: 2.421 ± 0.516
3.669ArgThr: 3.669 ± 0.434
3.889ArgVal: 3.889 ± 0.508
0.66ArgTrp: 0.66 ± 0.203
2.348ArgTyr: 2.348 ± 0.34
0.0ArgXaa: 0.0 ± 0.0
Ser
7.338SerAla: 7.338 ± 0.649
0.66SerCys: 0.66 ± 0.236
4.109SerAsp: 4.109 ± 0.529
3.155SerGlu: 3.155 ± 0.52
1.834SerPhe: 1.834 ± 0.324
5.944SerGly: 5.944 ± 0.604
0.881SerHis: 0.881 ± 0.3
2.715SerIle: 2.715 ± 0.524
3.889SerLys: 3.889 ± 0.656
4.403SerLeu: 4.403 ± 0.59
2.568SerMet: 2.568 ± 0.321
3.302SerAsn: 3.302 ± 0.539
2.642SerPro: 2.642 ± 0.391
1.908SerGln: 1.908 ± 0.381
2.348SerArg: 2.348 ± 0.395
2.862SerSer: 2.862 ± 0.557
4.916SerThr: 4.916 ± 0.768
4.549SerVal: 4.549 ± 0.494
1.027SerTrp: 1.027 ± 0.256
1.541SerTyr: 1.541 ± 0.39
0.0SerXaa: 0.0 ± 0.0
Thr
5.944ThrAla: 5.944 ± 0.899
0.66ThrCys: 0.66 ± 0.264
2.788ThrAsp: 2.788 ± 0.465
2.862ThrGlu: 2.862 ± 0.636
1.981ThrPhe: 1.981 ± 0.386
5.21ThrGly: 5.21 ± 0.717
0.954ThrHis: 0.954 ± 0.254
2.128ThrIle: 2.128 ± 0.376
2.348ThrLys: 2.348 ± 0.399
5.503ThrLeu: 5.503 ± 0.606
1.468ThrMet: 1.468 ± 0.334
1.761ThrAsn: 1.761 ± 0.387
2.788ThrPro: 2.788 ± 0.322
2.715ThrGln: 2.715 ± 0.475
2.715ThrArg: 2.715 ± 0.452
4.916ThrSer: 4.916 ± 0.681
3.596ThrThr: 3.596 ± 0.535
4.99ThrVal: 4.99 ± 0.665
0.881ThrTrp: 0.881 ± 0.222
2.201ThrTyr: 2.201 ± 0.559
0.0ThrXaa: 0.0 ± 0.0
Val
6.604ValAla: 6.604 ± 0.685
0.881ValCys: 0.881 ± 0.269
4.916ValAsp: 4.916 ± 0.573
4.623ValGlu: 4.623 ± 0.804
1.468ValPhe: 1.468 ± 0.258
6.457ValGly: 6.457 ± 0.653
1.834ValHis: 1.834 ± 0.361
2.568ValIle: 2.568 ± 0.422
3.302ValLys: 3.302 ± 0.529
5.577ValLeu: 5.577 ± 0.751
2.421ValMet: 2.421 ± 0.452
2.935ValAsn: 2.935 ± 0.581
3.229ValPro: 3.229 ± 0.521
3.742ValGln: 3.742 ± 0.677
4.403ValArg: 4.403 ± 0.487
4.476ValSer: 4.476 ± 0.638
3.375ValThr: 3.375 ± 0.551
5.503ValVal: 5.503 ± 0.613
0.66ValTrp: 0.66 ± 0.195
2.935ValTyr: 2.935 ± 0.43
0.0ValXaa: 0.0 ± 0.0
Trp
1.101TrpAla: 1.101 ± 0.283
0.22TrpCys: 0.22 ± 0.104
0.66TrpAsp: 0.66 ± 0.2
1.027TrpGlu: 1.027 ± 0.268
0.807TrpPhe: 0.807 ± 0.267
0.587TrpGly: 0.587 ± 0.215
0.22TrpHis: 0.22 ± 0.113
0.587TrpIle: 0.587 ± 0.244
0.66TrpLys: 0.66 ± 0.238
1.247TrpLeu: 1.247 ± 0.229
0.22TrpMet: 0.22 ± 0.136
0.881TrpAsn: 0.881 ± 0.264
0.367TrpPro: 0.367 ± 0.174
0.514TrpGln: 0.514 ± 0.175
0.807TrpArg: 0.807 ± 0.264
0.734TrpSer: 0.734 ± 0.262
1.027TrpThr: 1.027 ± 0.197
1.174TrpVal: 1.174 ± 0.272
0.367TrpTrp: 0.367 ± 0.168
0.587TrpTyr: 0.587 ± 0.217
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.642TyrAla: 2.642 ± 0.456
0.881TyrCys: 0.881 ± 0.25
2.568TyrAsp: 2.568 ± 0.41
1.981TyrGlu: 1.981 ± 0.575
1.321TyrPhe: 1.321 ± 0.281
2.715TyrGly: 2.715 ± 0.461
0.514TyrHis: 0.514 ± 0.196
1.908TyrIle: 1.908 ± 0.381
2.201TyrLys: 2.201 ± 0.378
4.036TyrLeu: 4.036 ± 0.55
0.881TyrMet: 0.881 ± 0.296
1.468TyrAsn: 1.468 ± 0.397
1.541TyrPro: 1.541 ± 0.372
1.981TyrGln: 1.981 ± 0.406
2.495TyrArg: 2.495 ± 0.524
2.642TyrSer: 2.642 ± 0.366
2.935TyrThr: 2.935 ± 0.537
2.128TyrVal: 2.128 ± 0.473
0.734TyrTrp: 0.734 ± 0.27
1.394TyrTyr: 1.394 ± 0.303
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (13629 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski