Gossypium australe

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 38125 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B6WY69|A0A5B6WY69_9ROSI Auxin-responsive protein OS=Gossypium australe OX=47621 GN=EPI10_030324 PE=3 SV=1
MM1 pKa = 7.25FSDD4 pKa = 3.75MSDD7 pKa = 4.01LPEE10 pKa = 4.06TWLSPTTLFLFLNIMVDD27 pKa = 3.68KK28 pKa = 10.62SWVLAGDD35 pKa = 3.53YY36 pKa = 10.93NIIANVEE43 pKa = 4.07EE44 pKa = 4.67SFDD47 pKa = 3.64PHH49 pKa = 6.82SCRR52 pKa = 11.84QFAGDD57 pKa = 3.75IFDD60 pKa = 4.68FHH62 pKa = 7.5DD63 pKa = 3.71CTFTT67 pKa = 3.69

Molecular weight: 7.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B6VTQ7|A0A5B6VTQ7_9ROSI Alkaline/neutral invertase OS=Gossypium australe OX=47621 GN=EPI10_023061 PE=3 SV=1
MM1 pKa = 7.35GLSSFLEE8 pKa = 4.05LSSRR12 pKa = 11.84SSTRR16 pKa = 11.84QRR18 pKa = 11.84TTQSSTNHH26 pKa = 5.01NNGYY30 pKa = 10.36KK31 pKa = 9.85SRR33 pKa = 11.84PWPFSLCPKK42 pKa = 9.05LTTDD46 pKa = 5.34AVYY49 pKa = 10.03TSNKK53 pKa = 9.15SRR55 pKa = 11.84SLGHH59 pKa = 6.02FLAVEE64 pKa = 4.3RR65 pKa = 11.84RR66 pKa = 11.84AATGNIGRR74 pKa = 11.84RR75 pKa = 11.84DD76 pKa = 3.66RR77 pKa = 11.84NSVGDD82 pKa = 3.91RR83 pKa = 11.84PVGDD87 pKa = 4.95LSPVMLNTEE96 pKa = 4.15QNSLFVSDD104 pKa = 5.77RR105 pKa = 11.84IAPRR109 pKa = 11.84IDD111 pKa = 2.98GRR113 pKa = 11.84KK114 pKa = 8.73SADD117 pKa = 3.31RR118 pKa = 11.84GVLEE122 pKa = 4.92HH123 pKa = 5.72GHH125 pKa = 6.29GYY127 pKa = 10.04GVPLLLSCLCRR138 pKa = 11.84QLMKK142 pKa = 10.93

Molecular weight: 15.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

34514

3611

38125

14614465

50

7283

383.3

43.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.367 ± 0.011

1.918 ± 0.006

5.206 ± 0.008

6.481 ± 0.017

4.393 ± 0.009

6.242 ± 0.011

2.333 ± 0.005

5.57 ± 0.009

6.452 ± 0.013

9.889 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.514 ± 0.005

4.541 ± 0.009

4.732 ± 0.012

3.707 ± 0.008

5.389 ± 0.01

8.633 ± 0.016

4.802 ± 0.008

6.562 ± 0.008

1.375 ± 0.005

2.896 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski