Amino acid dipepetide frequency for Cyanophage PSS2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.369AlaAla: 10.369 ± 1.311
1.562AlaCys: 1.562 ± 0.497
6.184AlaAsp: 6.184 ± 0.508
6.683AlaGlu: 6.683 ± 0.53
3.498AlaPhe: 3.498 ± 0.44
7.839AlaGly: 7.839 ± 0.625
1.374AlaHis: 1.374 ± 0.258
4.685AlaIle: 4.685 ± 0.537
5.059AlaLys: 5.059 ± 0.715
8.401AlaLeu: 8.401 ± 0.694
2.498AlaMet: 2.498 ± 0.503
3.904AlaAsn: 3.904 ± 0.392
4.31AlaPro: 4.31 ± 0.392
4.466AlaGln: 4.466 ± 0.501
3.81AlaArg: 3.81 ± 0.47
5.934AlaSer: 5.934 ± 0.618
6.184AlaThr: 6.184 ± 0.451
5.996AlaVal: 5.996 ± 0.433
1.156AlaTrp: 1.156 ± 0.225
2.655AlaTyr: 2.655 ± 0.26
0.0AlaXaa: 0.0 ± 0.0
Cys
0.999CysAla: 0.999 ± 0.336
0.437CysCys: 0.437 ± 0.206
0.906CysAsp: 0.906 ± 0.355
1.593CysGlu: 1.593 ± 0.857
0.531CysPhe: 0.531 ± 0.139
1.187CysGly: 1.187 ± 0.447
0.468CysHis: 0.468 ± 0.14
0.937CysIle: 0.937 ± 0.386
0.906CysLys: 0.906 ± 0.289
1.093CysLeu: 1.093 ± 0.251
0.219CysMet: 0.219 ± 0.081
0.874CysAsn: 0.874 ± 0.437
1.343CysPro: 1.343 ± 0.601
0.687CysGln: 0.687 ± 0.313
0.843CysArg: 0.843 ± 0.412
1.093CysSer: 1.093 ± 0.639
1.031CysThr: 1.031 ± 0.41
1.312CysVal: 1.312 ± 0.761
0.375CysTrp: 0.375 ± 0.122
0.5CysTyr: 0.5 ± 0.24
0.0CysXaa: 0.0 ± 0.0
Asp
6.964AspAla: 6.964 ± 0.619
1.999AspCys: 1.999 ± 1.135
5.559AspAsp: 5.559 ± 0.647
4.685AspGlu: 4.685 ± 0.487
2.498AspPhe: 2.498 ± 0.293
6.746AspGly: 6.746 ± 0.928
1.218AspHis: 1.218 ± 0.24
3.217AspIle: 3.217 ± 0.307
2.03AspLys: 2.03 ± 0.34
6.558AspLeu: 6.558 ± 0.567
1.593AspMet: 1.593 ± 0.296
2.186AspAsn: 2.186 ± 0.324
3.186AspPro: 3.186 ± 0.356
2.998AspGln: 2.998 ± 0.299
3.092AspArg: 3.092 ± 0.323
4.31AspSer: 4.31 ± 0.569
3.935AspThr: 3.935 ± 0.392
4.778AspVal: 4.778 ± 0.581
1.062AspTrp: 1.062 ± 0.195
1.999AspTyr: 1.999 ± 0.294
0.0AspXaa: 0.0 ± 0.0
Glu
7.714GluAla: 7.714 ± 0.56
1.187GluCys: 1.187 ± 0.618
3.623GluAsp: 3.623 ± 0.52
4.872GluGlu: 4.872 ± 0.467
2.311GluPhe: 2.311 ± 0.257
4.56GluGly: 4.56 ± 0.456
1.187GluHis: 1.187 ± 0.201
4.247GluIle: 4.247 ± 0.393
3.248GluLys: 3.248 ± 0.415
6.433GluLeu: 6.433 ± 0.678
1.655GluMet: 1.655 ± 0.24
2.311GluAsn: 2.311 ± 0.275
2.155GluPro: 2.155 ± 0.285
2.249GluGln: 2.249 ± 0.327
3.061GluArg: 3.061 ± 0.448
3.31GluSer: 3.31 ± 0.297
3.467GluThr: 3.467 ± 0.32
5.091GluVal: 5.091 ± 0.403
1.405GluTrp: 1.405 ± 0.252
1.874GluTyr: 1.874 ± 0.222
0.0GluXaa: 0.0 ± 0.0
Phe
2.873PheAla: 2.873 ± 0.305
0.687PheCys: 0.687 ± 0.311
2.904PheAsp: 2.904 ± 0.375
1.811PheGlu: 1.811 ± 0.202
1.374PhePhe: 1.374 ± 0.251
2.686PheGly: 2.686 ± 0.351
0.468PheHis: 0.468 ± 0.133
1.53PheIle: 1.53 ± 0.25
1.843PheLys: 1.843 ± 0.277
1.874PheLeu: 1.874 ± 0.262
0.812PheMet: 0.812 ± 0.158
1.343PheAsn: 1.343 ± 0.246
1.343PhePro: 1.343 ± 0.198
0.906PheGln: 0.906 ± 0.206
1.999PheArg: 1.999 ± 0.244
2.904PheSer: 2.904 ± 0.448
2.655PheThr: 2.655 ± 0.336
2.155PheVal: 2.155 ± 0.311
0.344PheTrp: 0.344 ± 0.101
0.999PheTyr: 0.999 ± 0.253
0.0PheXaa: 0.0 ± 0.0
Gly
6.152GlyAla: 6.152 ± 0.66
1.218GlyCys: 1.218 ± 0.436
5.434GlyAsp: 5.434 ± 0.72
4.716GlyGlu: 4.716 ± 0.658
2.748GlyPhe: 2.748 ± 0.339
7.995GlyGly: 7.995 ± 0.997
1.093GlyHis: 1.093 ± 0.199
3.404GlyIle: 3.404 ± 0.301
3.404GlyLys: 3.404 ± 0.468
6.527GlyLeu: 6.527 ± 0.557
1.718GlyMet: 1.718 ± 0.256
4.247GlyAsn: 4.247 ± 0.527
2.998GlyPro: 2.998 ± 0.348
3.529GlyGln: 3.529 ± 0.364
3.217GlyArg: 3.217 ± 0.39
6.59GlySer: 6.59 ± 0.633
6.402GlyThr: 6.402 ± 0.776
5.778GlyVal: 5.778 ± 0.468
1.811GlyTrp: 1.811 ± 0.281
3.373GlyTyr: 3.373 ± 0.334
0.0GlyXaa: 0.0 ± 0.0
His
1.093HisAla: 1.093 ± 0.195
0.406HisCys: 0.406 ± 0.177
1.28HisAsp: 1.28 ± 0.21
1.093HisGlu: 1.093 ± 0.209
0.874HisPhe: 0.874 ± 0.185
1.343HisGly: 1.343 ± 0.226
0.562HisHis: 0.562 ± 0.146
0.843HisIle: 0.843 ± 0.164
0.937HisLys: 0.937 ± 0.181
1.374HisLeu: 1.374 ± 0.251
0.312HisMet: 0.312 ± 0.097
0.843HisAsn: 0.843 ± 0.171
0.718HisPro: 0.718 ± 0.147
0.468HisGln: 0.468 ± 0.135
0.937HisArg: 0.937 ± 0.282
0.906HisSer: 0.906 ± 0.188
0.75HisThr: 0.75 ± 0.128
1.031HisVal: 1.031 ± 0.184
0.531HisTrp: 0.531 ± 0.118
0.718HisTyr: 0.718 ± 0.152
0.0HisXaa: 0.0 ± 0.0
Ile
4.341IleAla: 4.341 ± 0.445
0.874IleCys: 0.874 ± 0.314
4.31IleAsp: 4.31 ± 0.334
3.841IleGlu: 3.841 ± 0.294
1.156IlePhe: 1.156 ± 0.227
3.154IleGly: 3.154 ± 0.401
1.156IleHis: 1.156 ± 0.219
1.499IleIle: 1.499 ± 0.222
3.435IleLys: 3.435 ± 0.473
3.186IleLeu: 3.186 ± 0.329
0.562IleMet: 0.562 ± 0.125
2.53IleAsn: 2.53 ± 0.261
2.436IlePro: 2.436 ± 0.241
1.968IleGln: 1.968 ± 0.263
2.686IleArg: 2.686 ± 0.267
3.061IleSer: 3.061 ± 0.32
2.78IleThr: 2.78 ± 0.357
2.655IleVal: 2.655 ± 0.282
0.406IleTrp: 0.406 ± 0.13
1.437IleTyr: 1.437 ± 0.236
0.0IleXaa: 0.0 ± 0.0
Lys
5.215LysAla: 5.215 ± 0.799
0.625LysCys: 0.625 ± 0.272
2.842LysAsp: 2.842 ± 0.41
3.81LysGlu: 3.81 ± 0.626
1.562LysPhe: 1.562 ± 0.261
3.435LysGly: 3.435 ± 0.608
0.625LysHis: 0.625 ± 0.171
2.092LysIle: 2.092 ± 0.226
2.998LysLys: 2.998 ± 0.805
4.279LysLeu: 4.279 ± 0.505
0.874LysMet: 0.874 ± 0.211
1.874LysAsn: 1.874 ± 0.234
2.592LysPro: 2.592 ± 0.471
2.124LysGln: 2.124 ± 0.313
2.811LysArg: 2.811 ± 0.382
2.686LysSer: 2.686 ± 0.347
3.061LysThr: 3.061 ± 0.489
2.748LysVal: 2.748 ± 0.371
0.531LysTrp: 0.531 ± 0.103
0.906LysTyr: 0.906 ± 0.225
0.0LysXaa: 0.0 ± 0.0
Leu
7.62LeuAla: 7.62 ± 0.701
0.906LeuCys: 0.906 ± 0.228
6.152LeuAsp: 6.152 ± 0.492
5.871LeuGlu: 5.871 ± 0.444
1.78LeuPhe: 1.78 ± 0.27
5.465LeuGly: 5.465 ± 0.448
1.53LeuHis: 1.53 ± 0.28
3.904LeuIle: 3.904 ± 0.426
4.653LeuLys: 4.653 ± 0.562
6.433LeuLeu: 6.433 ± 0.583
1.624LeuMet: 1.624 ± 0.258
4.216LeuAsn: 4.216 ± 0.316
3.623LeuPro: 3.623 ± 0.398
3.217LeuGln: 3.217 ± 0.386
3.998LeuArg: 3.998 ± 0.366
5.778LeuSer: 5.778 ± 0.481
4.622LeuThr: 4.622 ± 0.461
4.747LeuVal: 4.747 ± 0.373
0.968LeuTrp: 0.968 ± 0.168
2.436LeuTyr: 2.436 ± 0.308
0.0LeuXaa: 0.0 ± 0.0
Met
2.748MetAla: 2.748 ± 0.337
0.094MetCys: 0.094 ± 0.049
1.437MetAsp: 1.437 ± 0.222
1.124MetGlu: 1.124 ± 0.237
0.562MetPhe: 0.562 ± 0.114
1.624MetGly: 1.624 ± 0.294
0.375MetHis: 0.375 ± 0.103
1.218MetIle: 1.218 ± 0.242
0.999MetLys: 0.999 ± 0.209
1.374MetLeu: 1.374 ± 0.254
0.344MetMet: 0.344 ± 0.117
1.031MetAsn: 1.031 ± 0.184
1.031MetPro: 1.031 ± 0.208
0.843MetGln: 0.843 ± 0.16
1.062MetArg: 1.062 ± 0.224
1.78MetSer: 1.78 ± 0.295
1.405MetThr: 1.405 ± 0.279
1.312MetVal: 1.312 ± 0.197
0.187MetTrp: 0.187 ± 0.073
0.531MetTyr: 0.531 ± 0.138
0.0MetXaa: 0.0 ± 0.0
Asn
4.591AsnAla: 4.591 ± 0.46
0.406AsnCys: 0.406 ± 0.159
2.655AsnAsp: 2.655 ± 0.374
2.03AsnGlu: 2.03 ± 0.24
1.374AsnPhe: 1.374 ± 0.248
5.028AsnGly: 5.028 ± 0.702
0.812AsnHis: 0.812 ± 0.159
1.874AsnIle: 1.874 ± 0.251
1.624AsnLys: 1.624 ± 0.428
3.029AsnLeu: 3.029 ± 0.357
0.75AsnMet: 0.75 ± 0.16
2.249AsnAsn: 2.249 ± 0.318
2.498AsnPro: 2.498 ± 0.296
1.749AsnGln: 1.749 ± 0.184
1.905AsnArg: 1.905 ± 0.228
3.56AsnSer: 3.56 ± 0.556
3.279AsnThr: 3.279 ± 0.403
2.936AsnVal: 2.936 ± 0.394
1.093AsnTrp: 1.093 ± 0.167
1.312AsnTyr: 1.312 ± 0.219
0.0AsnXaa: 0.0 ± 0.0
Pro
4.154ProAla: 4.154 ± 0.412
0.937ProCys: 0.937 ± 0.387
2.936ProAsp: 2.936 ± 0.35
3.186ProGlu: 3.186 ± 0.399
1.593ProPhe: 1.593 ± 0.22
4.154ProGly: 4.154 ± 0.506
0.437ProHis: 0.437 ± 0.125
2.124ProIle: 2.124 ± 0.245
1.843ProLys: 1.843 ± 0.34
3.029ProLeu: 3.029 ± 0.303
1.156ProMet: 1.156 ± 0.287
1.718ProAsn: 1.718 ± 0.251
2.061ProPro: 2.061 ± 0.281
1.499ProGln: 1.499 ± 0.231
1.343ProArg: 1.343 ± 0.207
3.467ProSer: 3.467 ± 0.354
3.592ProThr: 3.592 ± 0.409
2.717ProVal: 2.717 ± 0.373
0.562ProTrp: 0.562 ± 0.145
1.187ProTyr: 1.187 ± 0.2
0.0ProXaa: 0.0 ± 0.0
Gln
5.091GlnAla: 5.091 ± 0.41
0.593GlnCys: 0.593 ± 0.278
2.03GlnAsp: 2.03 ± 0.245
2.873GlnGlu: 2.873 ± 0.457
1.374GlnPhe: 1.374 ± 0.198
2.936GlnGly: 2.936 ± 0.328
0.718GlnHis: 0.718 ± 0.131
2.436GlnIle: 2.436 ± 0.331
1.593GlnLys: 1.593 ± 0.309
3.373GlnLeu: 3.373 ± 0.354
0.781GlnMet: 0.781 ± 0.19
1.468GlnAsn: 1.468 ± 0.162
1.031GlnPro: 1.031 ± 0.202
1.499GlnGln: 1.499 ± 0.233
1.968GlnArg: 1.968 ± 0.326
1.999GlnSer: 1.999 ± 0.252
2.686GlnThr: 2.686 ± 0.296
2.717GlnVal: 2.717 ± 0.243
0.656GlnTrp: 0.656 ± 0.128
0.937GlnTyr: 0.937 ± 0.162
0.0GlnXaa: 0.0 ± 0.0
Arg
4.31ArgAla: 4.31 ± 0.522
0.906ArgCys: 0.906 ± 0.253
2.873ArgAsp: 2.873 ± 0.289
2.904ArgGlu: 2.904 ± 0.464
2.03ArgPhe: 2.03 ± 0.296
3.404ArgGly: 3.404 ± 0.358
0.999ArgHis: 0.999 ± 0.198
2.249ArgIle: 2.249 ± 0.339
1.874ArgLys: 1.874 ± 0.303
4.466ArgLeu: 4.466 ± 0.414
1.312ArgMet: 1.312 ± 0.225
1.968ArgAsn: 1.968 ± 0.263
1.843ArgPro: 1.843 ± 0.287
1.968ArgGln: 1.968 ± 0.303
2.748ArgArg: 2.748 ± 0.421
2.78ArgSer: 2.78 ± 0.363
3.342ArgThr: 3.342 ± 0.298
2.78ArgVal: 2.78 ± 0.308
0.937ArgTrp: 0.937 ± 0.168
1.156ArgTyr: 1.156 ± 0.211
0.0ArgXaa: 0.0 ± 0.0
Ser
5.996SerAla: 5.996 ± 0.421
0.999SerCys: 0.999 ± 0.343
4.716SerAsp: 4.716 ± 0.816
4.154SerGlu: 4.154 ± 0.272
2.405SerPhe: 2.405 ± 0.283
6.746SerGly: 6.746 ± 0.593
0.999SerHis: 0.999 ± 0.191
3.31SerIle: 3.31 ± 0.493
2.998SerLys: 2.998 ± 0.358
4.716SerLeu: 4.716 ± 0.649
1.437SerMet: 1.437 ± 0.24
3.061SerAsn: 3.061 ± 0.427
3.061SerPro: 3.061 ± 0.372
2.748SerGln: 2.748 ± 0.309
2.717SerArg: 2.717 ± 0.324
5.497SerSer: 5.497 ± 0.606
5.497SerThr: 5.497 ± 0.551
4.528SerVal: 4.528 ± 0.378
1.249SerTrp: 1.249 ± 0.177
2.092SerTyr: 2.092 ± 0.321
0.0SerXaa: 0.0 ± 0.0
Thr
6.184ThrAla: 6.184 ± 0.537
1.218ThrCys: 1.218 ± 0.537
5.122ThrAsp: 5.122 ± 0.422
3.654ThrGlu: 3.654 ± 0.401
2.623ThrPhe: 2.623 ± 0.332
6.871ThrGly: 6.871 ± 0.649
1.093ThrHis: 1.093 ± 0.205
2.967ThrIle: 2.967 ± 0.439
2.592ThrLys: 2.592 ± 0.316
5.434ThrLeu: 5.434 ± 0.497
0.999ThrMet: 0.999 ± 0.194
3.404ThrAsn: 3.404 ± 0.335
3.404ThrPro: 3.404 ± 0.39
1.968ThrGln: 1.968 ± 0.304
2.436ThrArg: 2.436 ± 0.309
4.872ThrSer: 4.872 ± 0.424
5.059ThrThr: 5.059 ± 0.671
4.122ThrVal: 4.122 ± 0.384
1.405ThrTrp: 1.405 ± 0.287
2.061ThrTyr: 2.061 ± 0.285
0.0ThrXaa: 0.0 ± 0.0
Val
5.996ValAla: 5.996 ± 0.565
1.124ValCys: 1.124 ± 0.653
6.34ValAsp: 6.34 ± 0.597
4.56ValGlu: 4.56 ± 0.391
1.593ValPhe: 1.593 ± 0.273
4.029ValGly: 4.029 ± 0.406
1.062ValHis: 1.062 ± 0.222
3.029ValIle: 3.029 ± 0.401
3.342ValLys: 3.342 ± 0.414
4.403ValLeu: 4.403 ± 0.36
1.156ValMet: 1.156 ± 0.19
3.342ValAsn: 3.342 ± 0.364
2.467ValPro: 2.467 ± 0.291
2.186ValGln: 2.186 ± 0.34
3.748ValArg: 3.748 ± 0.421
5.059ValSer: 5.059 ± 0.45
4.279ValThr: 4.279 ± 0.519
4.185ValVal: 4.185 ± 0.447
1.249ValTrp: 1.249 ± 0.214
1.468ValTyr: 1.468 ± 0.23
0.0ValXaa: 0.0 ± 0.0
Trp
1.655TrpAla: 1.655 ± 0.242
0.375TrpCys: 0.375 ± 0.131
0.999TrpAsp: 0.999 ± 0.228
0.843TrpGlu: 0.843 ± 0.171
0.562TrpPhe: 0.562 ± 0.145
0.656TrpGly: 0.656 ± 0.158
0.312TrpHis: 0.312 ± 0.09
0.75TrpIle: 0.75 ± 0.181
0.906TrpLys: 0.906 ± 0.205
1.374TrpLeu: 1.374 ± 0.251
0.562TrpMet: 0.562 ± 0.134
0.843TrpAsn: 0.843 ± 0.155
0.468TrpPro: 0.468 ± 0.106
0.625TrpGln: 0.625 ± 0.134
0.968TrpArg: 0.968 ± 0.173
1.343TrpSer: 1.343 ± 0.249
1.437TrpThr: 1.437 ± 0.248
1.405TrpVal: 1.405 ± 0.225
0.25TrpTrp: 0.25 ± 0.074
0.437TrpTyr: 0.437 ± 0.128
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.498TyrAla: 2.498 ± 0.324
0.781TyrCys: 0.781 ± 0.282
2.311TyrAsp: 2.311 ± 0.303
1.655TyrGlu: 1.655 ± 0.221
1.062TyrPhe: 1.062 ± 0.242
2.498TyrGly: 2.498 ± 0.309
0.531TyrHis: 0.531 ± 0.13
1.156TyrIle: 1.156 ± 0.175
1.405TyrLys: 1.405 ± 0.241
2.186TyrLeu: 2.186 ± 0.319
0.687TyrMet: 0.687 ± 0.129
1.343TyrAsn: 1.343 ± 0.19
1.124TyrPro: 1.124 ± 0.196
1.062TyrGln: 1.062 ± 0.154
1.499TyrArg: 1.499 ± 0.233
2.03TyrSer: 2.03 ± 0.342
2.03TyrThr: 2.03 ± 0.3
1.624TyrVal: 1.624 ± 0.251
0.593TyrTrp: 0.593 ± 0.147
0.937TyrTyr: 0.937 ± 0.196
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 128 proteins (32021 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski