Thiothrix nivea (strain ATCC 35100 / DSM 5205 / JP2)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Thiotrichaceae; Thiothrix; Thiothrix nivea

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4218 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A656HF97|A0A656HF97_THINJ tRNA (cytidine(34)-2'-O)-methyltransferase OS=Thiothrix nivea (strain ATCC 35100 / DSM 5205 / JP2) OX=870187 GN=trmL PE=3 SV=1
MM1 pKa = 7.19SLKK4 pKa = 10.56RR5 pKa = 11.84LLLCSLLCISIPVYY19 pKa = 10.83AEE21 pKa = 3.46EE22 pKa = 4.64DD23 pKa = 3.73AEE25 pKa = 4.48SEE27 pKa = 4.1NLMTEE32 pKa = 4.59MAAKK36 pKa = 8.23MAQMKK41 pKa = 10.23ARR43 pKa = 11.84LRR45 pKa = 11.84AYY47 pKa = 9.37EE48 pKa = 4.1ADD50 pKa = 3.48VEE52 pKa = 4.49YY53 pKa = 10.9TNDD56 pKa = 3.24DD57 pKa = 3.63AYY59 pKa = 10.93AFSPFDD65 pKa = 3.46TTGCDD70 pKa = 3.09INIGNVIVDD79 pKa = 5.05DD80 pKa = 4.8GADD83 pKa = 3.61SPDD86 pKa = 3.56EE87 pKa = 4.4VIVLIDD93 pKa = 3.54GDD95 pKa = 4.3VIQSNNCRR103 pKa = 3.51

Molecular weight: 11.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A656HDU0|A0A656HDU0_THINJ von Willebrand factor type A OS=Thiothrix nivea (strain ATCC 35100 / DSM 5205 / JP2) OX=870187 GN=Thini_1771 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 8.0IHH11 pKa = 6.8RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.69GFRR19 pKa = 11.84ARR21 pKa = 11.84MATADD26 pKa = 3.43GRR28 pKa = 11.84KK29 pKa = 9.27VLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.3GRR39 pKa = 11.84ARR41 pKa = 11.84LIPP44 pKa = 4.0

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4218

0

4218

1343628

30

4992

318.5

35.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.862 ± 0.041

0.997 ± 0.014

5.553 ± 0.038

5.977 ± 0.041

3.781 ± 0.026

7.576 ± 0.048

2.392 ± 0.02

5.321 ± 0.033

4.358 ± 0.038

10.749 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.588 ± 0.021

3.714 ± 0.035

4.748 ± 0.031

4.654 ± 0.031

5.576 ± 0.038

5.656 ± 0.033

5.42 ± 0.047

6.745 ± 0.034

1.405 ± 0.015

2.928 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski