Amino acid dipepetide frequency for Mycobacterium phage Frankie

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.592AlaAla: 15.592 ± 1.759
0.947AlaCys: 0.947 ± 0.216
6.961AlaAsp: 6.961 ± 0.776
8.464AlaGlu: 8.464 ± 0.776
2.394AlaPhe: 2.394 ± 0.385
9.745AlaGly: 9.745 ± 1.064
2.951AlaHis: 2.951 ± 0.773
4.956AlaIle: 4.956 ± 0.573
4.288AlaLys: 4.288 ± 0.499
9.077AlaLeu: 9.077 ± 0.829
2.729AlaMet: 2.729 ± 0.45
3.453AlaAsn: 3.453 ± 0.577
5.123AlaPro: 5.123 ± 0.61
3.453AlaGln: 3.453 ± 0.488
6.515AlaArg: 6.515 ± 0.652
7.239AlaSer: 7.239 ± 0.691
6.794AlaThr: 6.794 ± 0.704
7.239AlaVal: 7.239 ± 0.63
2.784AlaTrp: 2.784 ± 0.399
3.174AlaTyr: 3.174 ± 0.449
0.0AlaXaa: 0.0 ± 0.0
Cys
1.114CysAla: 1.114 ± 0.274
0.223CysCys: 0.223 ± 0.102
1.169CysAsp: 1.169 ± 0.266
0.724CysGlu: 0.724 ± 0.183
0.167CysPhe: 0.167 ± 0.091
1.726CysGly: 1.726 ± 0.365
0.39CysHis: 0.39 ± 0.17
0.334CysIle: 0.334 ± 0.139
0.445CysLys: 0.445 ± 0.151
0.557CysLeu: 0.557 ± 0.187
0.111CysMet: 0.111 ± 0.079
0.334CysAsn: 0.334 ± 0.123
1.225CysPro: 1.225 ± 0.304
0.445CysGln: 0.445 ± 0.184
0.724CysArg: 0.724 ± 0.21
0.668CysSer: 0.668 ± 0.215
0.501CysThr: 0.501 ± 0.175
0.445CysVal: 0.445 ± 0.147
0.111CysTrp: 0.111 ± 0.084
0.167CysTyr: 0.167 ± 0.081
0.0CysXaa: 0.0 ± 0.0
Asp
7.128AspAla: 7.128 ± 0.582
0.78AspCys: 0.78 ± 0.185
4.789AspAsp: 4.789 ± 0.593
4.678AspGlu: 4.678 ± 0.648
1.281AspPhe: 1.281 ± 0.262
6.404AspGly: 6.404 ± 0.619
1.504AspHis: 1.504 ± 0.266
2.394AspIle: 2.394 ± 0.45
1.949AspLys: 1.949 ± 0.314
5.847AspLeu: 5.847 ± 0.5
1.336AspMet: 1.336 ± 0.266
1.559AspAsn: 1.559 ± 0.291
4.733AspPro: 4.733 ± 0.647
2.283AspGln: 2.283 ± 0.366
5.346AspArg: 5.346 ± 0.645
2.951AspSer: 2.951 ± 0.483
4.121AspThr: 4.121 ± 0.421
4.9AspVal: 4.9 ± 0.532
1.336AspTrp: 1.336 ± 0.281
1.949AspTyr: 1.949 ± 0.38
0.0AspXaa: 0.0 ± 0.0
Glu
6.961GluAla: 6.961 ± 0.714
0.78GluCys: 0.78 ± 0.221
3.508GluAsp: 3.508 ± 0.408
2.227GluGlu: 2.227 ± 0.342
1.949GluPhe: 1.949 ± 0.302
3.285GluGly: 3.285 ± 0.429
1.114GluHis: 1.114 ± 0.289
3.23GluIle: 3.23 ± 0.457
1.615GluLys: 1.615 ± 0.298
5.346GluLeu: 5.346 ± 0.574
1.615GluMet: 1.615 ± 0.288
1.615GluAsn: 1.615 ± 0.273
2.673GluPro: 2.673 ± 0.401
2.673GluGln: 2.673 ± 0.345
4.789GluArg: 4.789 ± 0.551
2.784GluSer: 2.784 ± 0.405
3.787GluThr: 3.787 ± 0.5
4.121GluVal: 4.121 ± 0.542
1.002GluTrp: 1.002 ± 0.254
1.559GluTyr: 1.559 ± 0.31
0.0GluXaa: 0.0 ± 0.0
Phe
2.84PheAla: 2.84 ± 0.379
0.223PheCys: 0.223 ± 0.103
2.394PheAsp: 2.394 ± 0.328
1.559PheGlu: 1.559 ± 0.248
0.947PhePhe: 0.947 ± 0.292
3.341PheGly: 3.341 ± 0.687
0.501PheHis: 0.501 ± 0.164
1.169PheIle: 1.169 ± 0.351
1.281PheLys: 1.281 ± 0.315
1.726PheLeu: 1.726 ± 0.259
0.78PheMet: 0.78 ± 0.253
0.891PheAsn: 0.891 ± 0.299
1.281PhePro: 1.281 ± 0.231
1.058PheGln: 1.058 ± 0.317
1.281PheArg: 1.281 ± 0.276
1.448PheSer: 1.448 ± 0.315
1.838PheThr: 1.838 ± 0.321
2.06PheVal: 2.06 ± 0.285
0.613PheTrp: 0.613 ± 0.165
0.78PheTyr: 0.78 ± 0.253
0.0PheXaa: 0.0 ± 0.0
Gly
9.912GlyAla: 9.912 ± 1.094
1.169GlyCys: 1.169 ± 0.279
5.68GlyAsp: 5.68 ± 0.62
3.285GlyGlu: 3.285 ± 0.498
2.784GlyPhe: 2.784 ± 0.45
8.91GlyGly: 8.91 ± 1.594
1.559GlyHis: 1.559 ± 0.301
3.731GlyIle: 3.731 ± 0.499
2.896GlyLys: 2.896 ± 0.303
5.958GlyLeu: 5.958 ± 0.595
2.339GlyMet: 2.339 ± 0.432
3.063GlyAsn: 3.063 ± 0.422
4.399GlyPro: 4.399 ± 0.752
2.339GlyGln: 2.339 ± 0.521
5.513GlyArg: 5.513 ± 0.562
5.513GlySer: 5.513 ± 0.785
5.736GlyThr: 5.736 ± 0.695
6.571GlyVal: 6.571 ± 0.656
2.172GlyTrp: 2.172 ± 0.364
2.339GlyTyr: 2.339 ± 0.33
0.0GlyXaa: 0.0 ± 0.0
His
2.227HisAla: 2.227 ± 0.41
0.334HisCys: 0.334 ± 0.149
1.058HisAsp: 1.058 ± 0.213
1.281HisGlu: 1.281 ± 0.243
0.557HisPhe: 0.557 ± 0.141
1.615HisGly: 1.615 ± 0.332
0.78HisHis: 0.78 ± 0.243
1.225HisIle: 1.225 ± 0.28
0.724HisLys: 0.724 ± 0.193
1.838HisLeu: 1.838 ± 0.364
0.39HisMet: 0.39 ± 0.146
0.78HisAsn: 0.78 ± 0.188
1.169HisPro: 1.169 ± 0.258
0.78HisGln: 0.78 ± 0.219
1.281HisArg: 1.281 ± 0.295
1.002HisSer: 1.002 ± 0.226
1.392HisThr: 1.392 ± 0.28
1.949HisVal: 1.949 ± 0.624
0.557HisTrp: 0.557 ± 0.161
0.891HisTyr: 0.891 ± 0.224
0.0HisXaa: 0.0 ± 0.0
Ile
6.07IleAla: 6.07 ± 0.593
0.223IleCys: 0.223 ± 0.124
3.62IleAsp: 3.62 ± 0.422
3.23IleGlu: 3.23 ± 0.427
1.002IlePhe: 1.002 ± 0.263
4.009IleGly: 4.009 ± 0.462
1.114IleHis: 1.114 ± 0.26
1.169IleIle: 1.169 ± 0.223
1.448IleLys: 1.448 ± 0.26
1.893IleLeu: 1.893 ± 0.444
0.501IleMet: 0.501 ± 0.172
2.394IleAsn: 2.394 ± 0.369
2.617IlePro: 2.617 ± 0.51
1.838IleGln: 1.838 ± 0.348
2.617IleArg: 2.617 ± 0.458
2.673IleSer: 2.673 ± 0.407
3.285IleThr: 3.285 ± 0.438
3.118IleVal: 3.118 ± 0.379
0.891IleTrp: 0.891 ± 0.229
0.891IleTyr: 0.891 ± 0.198
0.0IleXaa: 0.0 ± 0.0
Lys
4.566LysAla: 4.566 ± 0.501
0.223LysCys: 0.223 ± 0.113
1.782LysAsp: 1.782 ± 0.324
1.058LysGlu: 1.058 ± 0.233
1.002LysPhe: 1.002 ± 0.187
2.617LysGly: 2.617 ± 0.378
0.78LysHis: 0.78 ± 0.203
1.169LysIle: 1.169 ± 0.235
1.281LysLys: 1.281 ± 0.275
2.45LysLeu: 2.45 ± 0.439
0.445LysMet: 0.445 ± 0.142
1.114LysAsn: 1.114 ± 0.216
2.227LysPro: 2.227 ± 0.342
1.169LysGln: 1.169 ± 0.205
2.116LysArg: 2.116 ± 0.443
2.227LysSer: 2.227 ± 0.327
2.45LysThr: 2.45 ± 0.439
2.283LysVal: 2.283 ± 0.314
1.058LysTrp: 1.058 ± 0.242
0.835LysTyr: 0.835 ± 0.244
0.0LysXaa: 0.0 ± 0.0
Leu
8.631LeuAla: 8.631 ± 0.765
0.835LeuCys: 0.835 ± 0.2
5.067LeuAsp: 5.067 ± 0.542
3.564LeuGlu: 3.564 ± 0.422
2.283LeuPhe: 2.283 ± 0.295
5.457LeuGly: 5.457 ± 0.536
1.281LeuHis: 1.281 ± 0.239
3.564LeuIle: 3.564 ± 0.45
2.339LeuLys: 2.339 ± 0.415
5.179LeuLeu: 5.179 ± 0.646
1.392LeuMet: 1.392 ± 0.298
2.729LeuAsn: 2.729 ± 0.416
5.234LeuPro: 5.234 ± 0.632
2.84LeuGln: 2.84 ± 0.424
5.68LeuArg: 5.68 ± 0.628
4.399LeuSer: 4.399 ± 0.595
5.457LeuThr: 5.457 ± 0.542
5.457LeuVal: 5.457 ± 0.651
1.002LeuTrp: 1.002 ± 0.262
2.172LeuTyr: 2.172 ± 0.39
0.0LeuXaa: 0.0 ± 0.0
Met
2.06MetAla: 2.06 ± 0.396
0.334MetCys: 0.334 ± 0.185
1.169MetAsp: 1.169 ± 0.284
0.835MetGlu: 0.835 ± 0.16
0.557MetPhe: 0.557 ± 0.192
1.949MetGly: 1.949 ± 0.342
0.334MetHis: 0.334 ± 0.142
1.058MetIle: 1.058 ± 0.28
0.891MetLys: 0.891 ± 0.259
1.838MetLeu: 1.838 ± 0.287
0.668MetMet: 0.668 ± 0.232
0.891MetAsn: 0.891 ± 0.196
1.559MetPro: 1.559 ± 0.302
0.223MetGln: 0.223 ± 0.107
1.726MetArg: 1.726 ± 0.297
2.729MetSer: 2.729 ± 0.349
1.671MetThr: 1.671 ± 0.261
1.336MetVal: 1.336 ± 0.288
0.278MetTrp: 0.278 ± 0.132
0.334MetTyr: 0.334 ± 0.145
0.0MetXaa: 0.0 ± 0.0
Asn
3.453AsnAla: 3.453 ± 0.442
0.223AsnCys: 0.223 ± 0.105
1.782AsnAsp: 1.782 ± 0.292
1.281AsnGlu: 1.281 ± 0.269
0.724AsnPhe: 0.724 ± 0.277
3.787AsnGly: 3.787 ± 0.46
0.835AsnHis: 0.835 ± 0.174
1.671AsnIle: 1.671 ± 0.457
1.058AsnLys: 1.058 ± 0.236
2.506AsnLeu: 2.506 ± 0.405
0.835AsnMet: 0.835 ± 0.215
2.172AsnAsn: 2.172 ± 0.522
2.673AsnPro: 2.673 ± 0.374
1.336AsnGln: 1.336 ± 0.336
1.949AsnArg: 1.949 ± 0.354
1.392AsnSer: 1.392 ± 0.341
2.227AsnThr: 2.227 ± 0.344
2.005AsnVal: 2.005 ± 0.349
0.445AsnTrp: 0.445 ± 0.13
0.724AsnTyr: 0.724 ± 0.188
0.0AsnXaa: 0.0 ± 0.0
Pro
6.07ProAla: 6.07 ± 0.609
0.724ProCys: 0.724 ± 0.235
4.733ProAsp: 4.733 ± 0.499
4.455ProGlu: 4.455 ± 0.554
1.559ProPhe: 1.559 ± 0.311
5.958ProGly: 5.958 ± 0.655
1.559ProHis: 1.559 ± 0.294
2.172ProIle: 2.172 ± 0.337
1.782ProLys: 1.782 ± 0.299
4.121ProLeu: 4.121 ± 0.586
1.336ProMet: 1.336 ± 0.305
2.172ProAsn: 2.172 ± 0.331
3.731ProPro: 3.731 ± 0.513
2.673ProGln: 2.673 ± 0.428
3.23ProArg: 3.23 ± 0.439
3.341ProSer: 3.341 ± 0.416
3.954ProThr: 3.954 ± 0.49
4.511ProVal: 4.511 ± 0.442
0.947ProTrp: 0.947 ± 0.199
1.893ProTyr: 1.893 ± 0.302
0.0ProXaa: 0.0 ± 0.0
Gln
4.622GlnAla: 4.622 ± 0.603
0.334GlnCys: 0.334 ± 0.13
1.392GlnAsp: 1.392 ± 0.271
1.448GlnGlu: 1.448 ± 0.241
1.002GlnPhe: 1.002 ± 0.219
2.339GlnGly: 2.339 ± 0.496
0.891GlnHis: 0.891 ± 0.246
1.949GlnIle: 1.949 ± 0.396
1.114GlnLys: 1.114 ± 0.225
3.564GlnLeu: 3.564 ± 0.496
0.724GlnMet: 0.724 ± 0.224
0.835GlnAsn: 0.835 ± 0.312
2.394GlnPro: 2.394 ± 0.409
1.559GlnGln: 1.559 ± 0.476
2.45GlnArg: 2.45 ± 0.324
2.06GlnSer: 2.06 ± 0.388
1.448GlnThr: 1.448 ± 0.26
2.394GlnVal: 2.394 ± 0.385
0.78GlnTrp: 0.78 ± 0.169
1.169GlnTyr: 1.169 ± 0.29
0.0GlnXaa: 0.0 ± 0.0
Arg
6.794ArgAla: 6.794 ± 0.588
0.891ArgCys: 0.891 ± 0.314
4.956ArgAsp: 4.956 ± 0.585
4.288ArgGlu: 4.288 ± 0.566
2.283ArgPhe: 2.283 ± 0.362
4.343ArgGly: 4.343 ± 0.399
1.114ArgHis: 1.114 ± 0.322
3.787ArgIle: 3.787 ± 0.534
2.673ArgLys: 2.673 ± 0.386
5.123ArgLeu: 5.123 ± 0.53
2.45ArgMet: 2.45 ± 0.33
1.838ArgAsn: 1.838 ± 0.394
3.898ArgPro: 3.898 ± 0.567
2.005ArgGln: 2.005 ± 0.356
4.678ArgArg: 4.678 ± 0.635
4.288ArgSer: 4.288 ± 0.551
2.784ArgThr: 2.784 ± 0.454
5.067ArgVal: 5.067 ± 0.6
1.559ArgTrp: 1.559 ± 0.307
2.172ArgTyr: 2.172 ± 0.413
0.0ArgXaa: 0.0 ± 0.0
Ser
6.515SerAla: 6.515 ± 0.926
0.724SerCys: 0.724 ± 0.255
3.954SerAsp: 3.954 ± 0.494
3.174SerGlu: 3.174 ± 0.476
2.116SerPhe: 2.116 ± 0.315
5.624SerGly: 5.624 ± 0.71
1.058SerHis: 1.058 ± 0.225
2.84SerIle: 2.84 ± 0.466
1.893SerLys: 1.893 ± 0.358
3.954SerLeu: 3.954 ± 0.552
1.448SerMet: 1.448 ± 0.278
1.893SerAsn: 1.893 ± 0.381
3.731SerPro: 3.731 ± 0.43
1.448SerGln: 1.448 ± 0.26
4.399SerArg: 4.399 ± 0.495
3.787SerSer: 3.787 ± 0.569
3.898SerThr: 3.898 ± 0.532
4.789SerVal: 4.789 ± 0.502
1.559SerTrp: 1.559 ± 0.322
1.169SerTyr: 1.169 ± 0.227
0.0SerXaa: 0.0 ± 0.0
Thr
6.292ThrAla: 6.292 ± 0.587
0.78ThrCys: 0.78 ± 0.253
3.954ThrAsp: 3.954 ± 0.506
3.898ThrGlu: 3.898 ± 0.405
1.949ThrPhe: 1.949 ± 0.396
5.457ThrGly: 5.457 ± 0.687
1.504ThrHis: 1.504 ± 0.348
3.174ThrIle: 3.174 ± 0.448
1.671ThrLys: 1.671 ± 0.282
4.343ThrLeu: 4.343 ± 0.555
1.058ThrMet: 1.058 ± 0.201
1.782ThrAsn: 1.782 ± 0.354
4.566ThrPro: 4.566 ± 0.497
2.172ThrGln: 2.172 ± 0.355
3.842ThrArg: 3.842 ± 0.499
3.564ThrSer: 3.564 ± 0.483
3.842ThrThr: 3.842 ± 0.669
6.515ThrVal: 6.515 ± 0.647
1.058ThrTrp: 1.058 ± 0.227
1.559ThrTyr: 1.559 ± 0.317
0.0ThrXaa: 0.0 ± 0.0
Val
8.464ValAla: 8.464 ± 0.713
0.947ValCys: 0.947 ± 0.233
5.736ValAsp: 5.736 ± 0.685
4.455ValGlu: 4.455 ± 0.575
2.005ValPhe: 2.005 ± 0.409
5.791ValGly: 5.791 ± 0.637
1.726ValHis: 1.726 ± 0.293
3.174ValIle: 3.174 ± 0.442
2.005ValLys: 2.005 ± 0.42
5.847ValLeu: 5.847 ± 0.624
1.058ValMet: 1.058 ± 0.189
2.339ValAsn: 2.339 ± 0.338
4.845ValPro: 4.845 ± 0.463
2.394ValGln: 2.394 ± 0.346
4.9ValArg: 4.9 ± 0.629
5.179ValSer: 5.179 ± 0.451
5.012ValThr: 5.012 ± 0.513
6.014ValVal: 6.014 ± 0.762
1.615ValTrp: 1.615 ± 0.284
1.336ValTyr: 1.336 ± 0.288
0.0ValXaa: 0.0 ± 0.0
Trp
1.726TrpAla: 1.726 ± 0.302
0.445TrpCys: 0.445 ± 0.146
1.058TrpAsp: 1.058 ± 0.231
0.891TrpGlu: 0.891 ± 0.257
0.724TrpPhe: 0.724 ± 0.169
1.448TrpGly: 1.448 ± 0.349
0.501TrpHis: 0.501 ± 0.162
0.724TrpIle: 0.724 ± 0.17
0.668TrpLys: 0.668 ± 0.191
1.615TrpLeu: 1.615 ± 0.347
0.891TrpMet: 0.891 ± 0.254
0.39TrpAsn: 0.39 ± 0.178
1.225TrpPro: 1.225 ± 0.255
1.058TrpGln: 1.058 ± 0.246
1.893TrpArg: 1.893 ± 0.344
1.336TrpSer: 1.336 ± 0.257
1.504TrpThr: 1.504 ± 0.298
1.615TrpVal: 1.615 ± 0.452
0.891TrpTrp: 0.891 ± 0.216
0.445TrpTyr: 0.445 ± 0.151
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.84TyrAla: 2.84 ± 0.41
0.445TyrCys: 0.445 ± 0.156
2.506TyrAsp: 2.506 ± 0.406
1.838TyrGlu: 1.838 ± 0.352
0.724TyrPhe: 0.724 ± 0.206
2.06TyrGly: 2.06 ± 0.413
0.334TyrHis: 0.334 ± 0.122
1.114TyrIle: 1.114 ± 0.194
0.835TyrLys: 0.835 ± 0.227
1.782TyrLeu: 1.782 ± 0.32
0.278TyrMet: 0.278 ± 0.112
0.835TyrAsn: 0.835 ± 0.262
1.671TyrPro: 1.671 ± 0.275
0.724TyrGln: 0.724 ± 0.169
2.06TyrArg: 2.06 ± 0.353
1.392TyrSer: 1.392 ± 0.297
1.281TyrThr: 1.281 ± 0.331
2.45TyrVal: 2.45 ± 0.312
0.39TyrTrp: 0.39 ± 0.142
0.613TyrTyr: 0.613 ± 0.194
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 103 proteins (17959 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski