Alteromonas phage P24

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3T0ID36|A0A3T0ID36_9CAUD Uncharacterized protein OS=Alteromonas phage P24 OX=2500137 PE=4 SV=1
MM1 pKa = 6.69FTKK4 pKa = 10.61LVIKK8 pKa = 10.58QNGYY12 pKa = 10.44QYY14 pKa = 11.3LKK16 pKa = 11.18DD17 pKa = 3.84NVTVAHH23 pKa = 6.79LCKK26 pKa = 10.49NPSAADD32 pKa = 3.48SLATIQSKK40 pKa = 10.8SIATTPLAGTDD51 pKa = 3.5VSFSASGNDD60 pKa = 3.74LLVTINGKK68 pKa = 10.06SGIDD72 pKa = 3.55PSGVAASTDD81 pKa = 3.9DD82 pKa = 3.54IQIVYY87 pKa = 9.96CSASEE92 pKa = 4.07VLVSVDD98 pKa = 3.94ANDD101 pKa = 4.29RR102 pKa = 11.84DD103 pKa = 3.8ITNEE107 pKa = 4.16DD108 pKa = 3.93GDD110 pKa = 4.49TVDD113 pKa = 4.72IPAAQIYY120 pKa = 9.4VRR122 pKa = 11.84EE123 pKa = 4.06ISAVV127 pKa = 3.29

Molecular weight: 13.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H0QUC7|A0A6H0QUC7_9CAUD Uncharacterized protein OS=Alteromonas phage P24 OX=2500137 PE=4 SV=1
MM1 pKa = 7.32SLVSVVSVSHH11 pKa = 5.74SSKK14 pKa = 10.63RR15 pKa = 11.84YY16 pKa = 9.89CCWLWANEE24 pKa = 3.82GAHH27 pKa = 5.68YY28 pKa = 9.8KK29 pKa = 9.8PHH31 pKa = 6.62GINRR35 pKa = 11.84QSHH38 pKa = 5.46GPEE41 pKa = 3.6NVRR44 pKa = 11.84IRR46 pKa = 11.84HH47 pKa = 4.57GTGNAFPSLYY57 pKa = 10.15EE58 pKa = 4.1SQFYY62 pKa = 8.17TLWLGLINKK71 pKa = 8.84RR72 pKa = 11.84VSVRR76 pKa = 11.84QLTGKK81 pKa = 10.08RR82 pKa = 11.84RR83 pKa = 11.84LTPIISGRR91 pKa = 11.84ARR93 pKa = 11.84HH94 pKa = 5.62VRR96 pKa = 11.84LSLQPRR102 pKa = 11.84LTHH105 pKa = 7.06LEE107 pKa = 3.83QTGYY111 pKa = 8.92QQ112 pKa = 3.35

Molecular weight: 12.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

14445

34

1560

195.2

21.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.138 ± 0.431

0.893 ± 0.141

5.587 ± 0.236

6.771 ± 0.267

3.731 ± 0.212

6.535 ± 0.269

1.938 ± 0.218

6.307 ± 0.299

6.604 ± 0.476

7.996 ± 0.25

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.34 ± 0.224

5.199 ± 0.231

3.434 ± 0.158

4.451 ± 0.179

4.888 ± 0.281

7.525 ± 0.281

5.933 ± 0.341

6.369 ± 0.194

1.26 ± 0.107

3.101 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski