Azospirillum baldaniorum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Azospirillaceae; Azospirillum

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7541 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8AYJ0|G8AYJ0_9PROT Uncharacterized protein OS=Azospirillum baldaniorum OX=1064539 GN=AZOBR_p340187 PE=4 SV=1
MM1 pKa = 7.65NEE3 pKa = 3.97EE4 pKa = 4.1FQTAAADD11 pKa = 3.44SGLAIQGAINRR22 pKa = 11.84LGLYY26 pKa = 9.58IGSPDD31 pKa = 3.94IYY33 pKa = 10.21PGLPVQEE40 pKa = 4.32PVVPAPAGRR49 pKa = 11.84PIALAQQAALDD60 pKa = 3.93AVAAAIAGVAALLVDD75 pKa = 4.02MVGDD79 pKa = 3.76PAVTPYY85 pKa = 10.08EE86 pKa = 4.25VPGLSLPGADD96 pKa = 2.8VATYY100 pKa = 10.53LGLITSEE107 pKa = 4.58HH108 pKa = 6.62ASQPLYY114 pKa = 10.44RR115 pKa = 11.84AALTALLKK123 pKa = 10.4PLSDD127 pKa = 3.86GQGSFAGLADD137 pKa = 4.35RR138 pKa = 11.84FDD140 pKa = 4.71LDD142 pKa = 4.27RR143 pKa = 11.84AVGDD147 pKa = 3.58QLDD150 pKa = 4.47TIGLWVGASRR160 pKa = 11.84RR161 pKa = 11.84LTVPLTGVYY170 pKa = 10.4FSFDD174 pKa = 3.22TAGVGFDD181 pKa = 3.53QGSWWAVGDD190 pKa = 3.55PTGYY194 pKa = 8.88LTVLPDD200 pKa = 3.65DD201 pKa = 5.16GYY203 pKa = 10.05RR204 pKa = 11.84TLIRR208 pKa = 11.84ARR210 pKa = 11.84IAANAWDD217 pKa = 3.89GTIPGAYY224 pKa = 9.4AVWDD228 pKa = 3.4IAFAGTGYY236 pKa = 10.75SVLIQDD242 pKa = 4.38NQNMTMDD249 pKa = 4.58FALLGPSPDD258 pKa = 3.56AVTLALFTGGYY269 pKa = 10.14LNVRR273 pKa = 11.84PAGVAVNAYY282 pKa = 5.57WTQSVPDD289 pKa = 3.73TPYY292 pKa = 10.78FGFDD296 pKa = 3.25AADD299 pKa = 3.36SGIAGFDD306 pKa = 3.18VGAWGLPTSPP316 pKa = 4.65

Molecular weight: 32.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8AI80|G8AI80_9PROT Glycosyl transferase OS=Azospirillum baldaniorum OX=1064539 GN=AZOBR_40152 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 8.96IVRR12 pKa = 11.84KK13 pKa = 9.24RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.3VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.29GRR39 pKa = 11.84SVLSAA44 pKa = 3.74

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7541

0

7541

2208573

13

3900

292.9

31.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.129 ± 0.041

0.87 ± 0.01

5.701 ± 0.022

5.375 ± 0.029

3.323 ± 0.02

9.135 ± 0.035

2.117 ± 0.018

4.23 ± 0.02

2.677 ± 0.022

10.446 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.336 ± 0.015

2.398 ± 0.023

5.788 ± 0.029

3.005 ± 0.018

8.009 ± 0.044

5.024 ± 0.027

5.501 ± 0.048

7.667 ± 0.025

1.324 ± 0.015

1.945 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski