Amino acid dipepetide frequency for Pseudoalteromonas phage Pq0

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.58AlaAla: 8.58 ± 2.126
0.499AlaCys: 0.499 ± 0.259
4.989AlaAsp: 4.989 ± 0.895
5.787AlaGlu: 5.787 ± 1.107
3.093AlaPhe: 3.093 ± 0.59
5.188AlaGly: 5.188 ± 0.829
1.197AlaHis: 1.197 ± 0.419
5.787AlaIle: 5.787 ± 0.909
6.585AlaLys: 6.585 ± 0.864
7.583AlaLeu: 7.583 ± 0.879
2.794AlaMet: 2.794 ± 0.625
3.592AlaAsn: 3.592 ± 0.558
2.394AlaPro: 2.394 ± 0.542
2.993AlaGln: 2.993 ± 0.518
3.791AlaArg: 3.791 ± 0.713
5.687AlaSer: 5.687 ± 0.661
5.088AlaThr: 5.088 ± 0.751
5.986AlaVal: 5.986 ± 0.649
0.998AlaTrp: 0.998 ± 0.325
2.095AlaTyr: 2.095 ± 0.453
0.0AlaXaa: 0.0 ± 0.0
Cys
0.798CysAla: 0.798 ± 0.307
0.2CysCys: 0.2 ± 0.151
0.898CysAsp: 0.898 ± 0.272
0.299CysGlu: 0.299 ± 0.145
0.2CysPhe: 0.2 ± 0.139
0.399CysGly: 0.399 ± 0.184
0.0CysHis: 0.0 ± 0.0
0.399CysIle: 0.399 ± 0.2
0.998CysLys: 0.998 ± 0.291
1.197CysLeu: 1.197 ± 0.322
0.2CysMet: 0.2 ± 0.134
0.599CysAsn: 0.599 ± 0.315
0.499CysPro: 0.499 ± 0.218
0.499CysGln: 0.499 ± 0.198
0.898CysArg: 0.898 ± 0.264
0.798CysSer: 0.798 ± 0.324
0.798CysThr: 0.798 ± 0.304
0.599CysVal: 0.599 ± 0.337
0.1CysTrp: 0.1 ± 0.088
0.399CysTyr: 0.399 ± 0.199
0.0CysXaa: 0.0 ± 0.0
Asp
4.19AspAla: 4.19 ± 0.647
0.698AspCys: 0.698 ± 0.274
4.091AspAsp: 4.091 ± 0.559
4.589AspGlu: 4.589 ± 0.789
2.295AspPhe: 2.295 ± 0.386
4.589AspGly: 4.589 ± 0.634
0.599AspHis: 0.599 ± 0.211
4.39AspIle: 4.39 ± 0.671
3.692AspLys: 3.692 ± 0.734
5.487AspLeu: 5.487 ± 0.9
1.796AspMet: 1.796 ± 0.442
3.592AspAsn: 3.592 ± 0.557
1.995AspPro: 1.995 ± 0.489
1.696AspGln: 1.696 ± 0.484
2.394AspArg: 2.394 ± 0.431
5.288AspSer: 5.288 ± 0.615
2.794AspThr: 2.794 ± 0.611
3.193AspVal: 3.193 ± 0.496
0.998AspTrp: 0.998 ± 0.294
3.193AspTyr: 3.193 ± 0.54
0.0AspXaa: 0.0 ± 0.0
Glu
5.388GluAla: 5.388 ± 0.924
0.599GluCys: 0.599 ± 0.23
3.193GluAsp: 3.193 ± 0.57
3.292GluGlu: 3.292 ± 0.487
2.594GluPhe: 2.594 ± 0.456
2.893GluGly: 2.893 ± 0.452
0.998GluHis: 0.998 ± 0.297
5.388GluIle: 5.388 ± 0.71
5.886GluLys: 5.886 ± 1.116
8.48GluLeu: 8.48 ± 1.069
2.195GluMet: 2.195 ± 0.456
3.891GluAsn: 3.891 ± 0.573
1.197GluPro: 1.197 ± 0.361
3.592GluGln: 3.592 ± 0.685
3.991GluArg: 3.991 ± 0.728
4.19GluSer: 4.19 ± 0.57
3.392GluThr: 3.392 ± 0.608
3.891GluVal: 3.891 ± 0.58
1.297GluTrp: 1.297 ± 0.333
2.394GluTyr: 2.394 ± 0.478
0.0GluXaa: 0.0 ± 0.0
Phe
3.093PheAla: 3.093 ± 0.656
0.399PheCys: 0.399 ± 0.198
2.494PheAsp: 2.494 ± 0.565
2.993PheGlu: 2.993 ± 0.647
1.297PhePhe: 1.297 ± 0.383
2.794PheGly: 2.794 ± 0.507
0.499PheHis: 0.499 ± 0.209
1.995PheIle: 1.995 ± 0.443
2.893PheLys: 2.893 ± 0.576
1.896PheLeu: 1.896 ± 0.381
0.798PheMet: 0.798 ± 0.293
2.794PheAsn: 2.794 ± 0.464
0.599PhePro: 0.599 ± 0.244
0.599PheGln: 0.599 ± 0.179
1.297PheArg: 1.297 ± 0.339
2.594PheSer: 2.594 ± 0.392
2.794PheThr: 2.794 ± 0.605
2.195PheVal: 2.195 ± 0.41
0.299PheTrp: 0.299 ± 0.149
1.796PheTyr: 1.796 ± 0.524
0.0PheXaa: 0.0 ± 0.0
Gly
6.385GlyAla: 6.385 ± 1.033
0.299GlyCys: 0.299 ± 0.173
3.991GlyAsp: 3.991 ± 0.712
4.689GlyGlu: 4.689 ± 0.767
2.494GlyPhe: 2.494 ± 0.473
6.186GlyGly: 6.186 ± 1.661
0.399GlyHis: 0.399 ± 0.171
3.492GlyIle: 3.492 ± 0.648
4.889GlyLys: 4.889 ± 0.661
6.186GlyLeu: 6.186 ± 0.672
1.297GlyMet: 1.297 ± 0.397
3.492GlyAsn: 3.492 ± 0.641
0.2GlyPro: 0.2 ± 0.139
2.594GlyGln: 2.594 ± 0.444
2.295GlyArg: 2.295 ± 0.442
4.091GlySer: 4.091 ± 0.765
4.091GlyThr: 4.091 ± 0.799
5.587GlyVal: 5.587 ± 0.848
0.499GlyTrp: 0.499 ± 0.224
2.893GlyTyr: 2.893 ± 0.55
0.0GlyXaa: 0.0 ± 0.0
His
0.998HisAla: 0.998 ± 0.334
0.1HisCys: 0.1 ± 0.103
0.798HisAsp: 0.798 ± 0.517
0.798HisGlu: 0.798 ± 0.268
0.299HisPhe: 0.299 ± 0.228
0.499HisGly: 0.499 ± 0.244
0.299HisHis: 0.299 ± 0.156
0.998HisIle: 0.998 ± 0.326
1.197HisLys: 1.197 ± 0.388
0.898HisLeu: 0.898 ± 0.347
0.0HisMet: 0.0 ± 0.0
0.998HisAsn: 0.998 ± 0.324
0.399HisPro: 0.399 ± 0.181
0.698HisGln: 0.698 ± 0.261
0.798HisArg: 0.798 ± 0.311
0.798HisSer: 0.798 ± 0.243
0.798HisThr: 0.798 ± 0.333
0.798HisVal: 0.798 ± 0.326
0.0HisTrp: 0.0 ± 0.0
0.499HisTyr: 0.499 ± 0.21
0.0HisXaa: 0.0 ± 0.0
Ile
4.889IleAla: 4.889 ± 0.722
0.599IleCys: 0.599 ± 0.296
4.989IleAsp: 4.989 ± 0.636
5.188IleGlu: 5.188 ± 0.836
1.197IlePhe: 1.197 ± 0.34
5.487IleGly: 5.487 ± 0.741
0.698IleHis: 0.698 ± 0.276
2.794IleIle: 2.794 ± 0.526
5.986IleLys: 5.986 ± 1.109
4.29IleLeu: 4.29 ± 0.736
1.596IleMet: 1.596 ± 0.462
3.991IleAsn: 3.991 ± 0.68
2.494IlePro: 2.494 ± 0.475
1.896IleGln: 1.896 ± 0.38
3.292IleArg: 3.292 ± 0.519
4.39IleSer: 4.39 ± 0.815
4.29IleThr: 4.29 ± 0.819
3.592IleVal: 3.592 ± 0.577
0.499IleTrp: 0.499 ± 0.195
1.796IleTyr: 1.796 ± 0.413
0.0IleXaa: 0.0 ± 0.0
Lys
6.385LysAla: 6.385 ± 0.948
0.798LysCys: 0.798 ± 0.257
4.49LysAsp: 4.49 ± 0.597
4.889LysGlu: 4.889 ± 0.82
2.095LysPhe: 2.095 ± 0.502
4.19LysGly: 4.19 ± 0.722
0.898LysHis: 0.898 ± 0.339
4.689LysIle: 4.689 ± 0.843
5.088LysLys: 5.088 ± 0.825
6.784LysLeu: 6.784 ± 0.916
1.995LysMet: 1.995 ± 0.501
4.589LysAsn: 4.589 ± 0.691
2.195LysPro: 2.195 ± 0.471
3.692LysGln: 3.692 ± 0.765
4.091LysArg: 4.091 ± 0.78
5.587LysSer: 5.587 ± 0.743
3.292LysThr: 3.292 ± 0.47
4.19LysVal: 4.19 ± 0.632
0.898LysTrp: 0.898 ± 0.298
2.394LysTyr: 2.394 ± 0.564
0.0LysXaa: 0.0 ± 0.0
Leu
7.882LeuAla: 7.882 ± 0.894
1.097LeuCys: 1.097 ± 0.352
4.091LeuAsp: 4.091 ± 0.567
6.186LeuGlu: 6.186 ± 0.731
2.195LeuPhe: 2.195 ± 0.351
5.388LeuGly: 5.388 ± 0.648
0.599LeuHis: 0.599 ± 0.241
5.886LeuIle: 5.886 ± 0.68
6.784LeuLys: 6.784 ± 0.777
4.689LeuLeu: 4.689 ± 0.818
2.993LeuMet: 2.993 ± 0.598
4.49LeuAsn: 4.49 ± 0.724
2.794LeuPro: 2.794 ± 0.58
3.492LeuGln: 3.492 ± 0.502
2.993LeuArg: 2.993 ± 0.549
7.682LeuSer: 7.682 ± 1.025
4.49LeuThr: 4.49 ± 0.551
3.692LeuVal: 3.692 ± 0.595
1.097LeuTrp: 1.097 ± 0.264
2.893LeuTyr: 2.893 ± 0.512
0.0LeuXaa: 0.0 ± 0.0
Met
2.594MetAla: 2.594 ± 0.708
0.2MetCys: 0.2 ± 0.141
0.599MetAsp: 0.599 ± 0.226
1.397MetGlu: 1.397 ± 0.454
0.998MetPhe: 0.998 ± 0.301
1.497MetGly: 1.497 ± 0.343
0.698MetHis: 0.698 ± 0.236
1.896MetIle: 1.896 ± 0.448
2.195MetLys: 2.195 ± 0.512
2.394MetLeu: 2.394 ± 0.481
0.499MetMet: 0.499 ± 0.226
1.696MetAsn: 1.696 ± 0.451
0.798MetPro: 0.798 ± 0.315
1.796MetGln: 1.796 ± 0.389
1.596MetArg: 1.596 ± 0.441
2.594MetSer: 2.594 ± 0.625
1.995MetThr: 1.995 ± 0.51
1.097MetVal: 1.097 ± 0.28
0.2MetTrp: 0.2 ± 0.123
0.499MetTyr: 0.499 ± 0.189
0.0MetXaa: 0.0 ± 0.0
Asn
4.889AsnAla: 4.889 ± 0.658
0.698AsnCys: 0.698 ± 0.258
4.39AsnAsp: 4.39 ± 0.588
3.791AsnGlu: 3.791 ± 0.467
2.494AsnPhe: 2.494 ± 0.391
4.589AsnGly: 4.589 ± 0.658
0.798AsnHis: 0.798 ± 0.337
3.692AsnIle: 3.692 ± 0.602
4.49AsnLys: 4.49 ± 0.721
4.889AsnLeu: 4.889 ± 0.606
1.596AsnMet: 1.596 ± 0.547
2.694AsnAsn: 2.694 ± 0.517
2.694AsnPro: 2.694 ± 0.585
2.195AsnGln: 2.195 ± 0.514
1.896AsnArg: 1.896 ± 0.468
3.692AsnSer: 3.692 ± 0.558
2.594AsnThr: 2.594 ± 0.473
3.193AsnVal: 3.193 ± 0.712
0.698AsnTrp: 0.698 ± 0.252
1.596AsnTyr: 1.596 ± 0.354
0.0AsnXaa: 0.0 ± 0.0
Pro
2.394ProAla: 2.394 ± 0.495
0.2ProCys: 0.2 ± 0.163
1.796ProAsp: 1.796 ± 0.445
1.696ProGlu: 1.696 ± 0.482
1.796ProPhe: 1.796 ± 0.502
0.798ProGly: 0.798 ± 0.263
0.2ProHis: 0.2 ± 0.13
1.696ProIle: 1.696 ± 0.436
1.896ProLys: 1.896 ± 0.361
2.195ProLeu: 2.195 ± 0.414
1.097ProMet: 1.097 ± 0.328
2.394ProAsn: 2.394 ± 0.642
0.798ProPro: 0.798 ± 0.272
1.796ProGln: 1.796 ± 0.48
0.898ProArg: 0.898 ± 0.312
2.494ProSer: 2.494 ± 0.666
2.095ProThr: 2.095 ± 0.468
2.494ProVal: 2.494 ± 0.467
0.399ProTrp: 0.399 ± 0.182
0.898ProTyr: 0.898 ± 0.329
0.0ProXaa: 0.0 ± 0.0
Gln
4.29GlnAla: 4.29 ± 0.796
0.499GlnCys: 0.499 ± 0.264
2.195GlnAsp: 2.195 ± 0.447
2.794GlnGlu: 2.794 ± 0.546
0.998GlnPhe: 0.998 ± 0.325
1.995GlnGly: 1.995 ± 0.367
0.898GlnHis: 0.898 ± 0.287
2.394GlnIle: 2.394 ± 0.482
2.494GlnLys: 2.494 ± 0.456
3.692GlnLeu: 3.692 ± 0.768
1.297GlnMet: 1.297 ± 0.459
2.195GlnAsn: 2.195 ± 0.373
1.297GlnPro: 1.297 ± 0.378
2.594GlnGln: 2.594 ± 0.997
2.494GlnArg: 2.494 ± 0.543
3.692GlnSer: 3.692 ± 0.566
2.394GlnThr: 2.394 ± 0.455
2.594GlnVal: 2.594 ± 0.377
0.898GlnTrp: 0.898 ± 0.368
0.798GlnTyr: 0.798 ± 0.288
0.0GlnXaa: 0.0 ± 0.0
Arg
3.891ArgAla: 3.891 ± 0.443
0.698ArgCys: 0.698 ± 0.269
3.193ArgAsp: 3.193 ± 0.519
3.592ArgGlu: 3.592 ± 0.787
1.796ArgPhe: 1.796 ± 0.48
2.195ArgGly: 2.195 ± 0.459
0.698ArgHis: 0.698 ± 0.318
3.692ArgIle: 3.692 ± 0.783
2.993ArgLys: 2.993 ± 0.603
4.49ArgLeu: 4.49 ± 0.559
1.097ArgMet: 1.097 ± 0.309
2.095ArgAsn: 2.095 ± 0.412
0.998ArgPro: 0.998 ± 0.339
1.995ArgGln: 1.995 ± 0.438
1.896ArgArg: 1.896 ± 0.489
2.295ArgSer: 2.295 ± 0.493
2.095ArgThr: 2.095 ± 0.392
2.594ArgVal: 2.594 ± 0.471
0.798ArgTrp: 0.798 ± 0.245
1.596ArgTyr: 1.596 ± 0.397
0.0ArgXaa: 0.0 ± 0.0
Ser
5.188SerAla: 5.188 ± 0.835
0.599SerCys: 0.599 ± 0.241
4.29SerAsp: 4.29 ± 0.661
4.989SerGlu: 4.989 ± 0.605
3.791SerPhe: 3.791 ± 0.556
6.186SerGly: 6.186 ± 0.975
1.197SerHis: 1.197 ± 0.394
4.39SerIle: 4.39 ± 0.573
5.587SerLys: 5.587 ± 0.874
5.188SerLeu: 5.188 ± 0.787
1.197SerMet: 1.197 ± 0.333
3.891SerAsn: 3.891 ± 0.584
2.893SerPro: 2.893 ± 0.507
3.791SerGln: 3.791 ± 0.594
2.594SerArg: 2.594 ± 0.54
4.29SerSer: 4.29 ± 0.868
3.891SerThr: 3.891 ± 0.551
5.587SerVal: 5.587 ± 0.878
0.998SerTrp: 0.998 ± 0.382
2.195SerTyr: 2.195 ± 0.455
0.0SerXaa: 0.0 ± 0.0
Thr
4.889ThrAla: 4.889 ± 0.602
0.499ThrCys: 0.499 ± 0.245
3.791ThrAsp: 3.791 ± 0.596
3.791ThrGlu: 3.791 ± 0.527
2.295ThrPhe: 2.295 ± 0.478
4.789ThrGly: 4.789 ± 0.748
0.998ThrHis: 0.998 ± 0.262
3.891ThrIle: 3.891 ± 0.615
4.39ThrLys: 4.39 ± 0.528
3.592ThrLeu: 3.592 ± 0.465
1.097ThrMet: 1.097 ± 0.359
2.694ThrAsn: 2.694 ± 0.499
2.295ThrPro: 2.295 ± 0.464
2.295ThrGln: 2.295 ± 0.494
1.796ThrArg: 1.796 ± 0.405
3.592ThrSer: 3.592 ± 0.605
3.292ThrThr: 3.292 ± 0.546
4.589ThrVal: 4.589 ± 0.76
0.898ThrTrp: 0.898 ± 0.26
1.696ThrTyr: 1.696 ± 0.342
0.0ThrXaa: 0.0 ± 0.0
Val
4.589ValAla: 4.589 ± 0.656
0.898ValCys: 0.898 ± 0.346
4.39ValAsp: 4.39 ± 0.64
4.49ValGlu: 4.49 ± 0.448
2.295ValPhe: 2.295 ± 0.485
3.592ValGly: 3.592 ± 0.537
0.798ValHis: 0.798 ± 0.25
3.592ValIle: 3.592 ± 0.498
3.292ValLys: 3.292 ± 0.682
4.091ValLeu: 4.091 ± 0.703
2.095ValMet: 2.095 ± 0.483
4.589ValAsn: 4.589 ± 0.747
2.394ValPro: 2.394 ± 0.699
1.896ValGln: 1.896 ± 0.405
3.592ValArg: 3.592 ± 0.613
4.989ValSer: 4.989 ± 0.767
4.589ValThr: 4.589 ± 0.788
2.794ValVal: 2.794 ± 0.454
0.399ValTrp: 0.399 ± 0.207
2.694ValTyr: 2.694 ± 0.633
0.0ValXaa: 0.0 ± 0.0
Trp
0.599TrpAla: 0.599 ± 0.242
0.2TrpCys: 0.2 ± 0.124
0.898TrpAsp: 0.898 ± 0.294
0.599TrpGlu: 0.599 ± 0.219
0.599TrpPhe: 0.599 ± 0.239
0.499TrpGly: 0.499 ± 0.221
0.0TrpHis: 0.0 ± 0.0
0.998TrpIle: 0.998 ± 0.231
0.499TrpLys: 0.499 ± 0.212
1.097TrpLeu: 1.097 ± 0.301
0.399TrpMet: 0.399 ± 0.22
0.499TrpAsn: 0.499 ± 0.222
0.399TrpPro: 0.399 ± 0.218
0.898TrpGln: 0.898 ± 0.282
0.798TrpArg: 0.798 ± 0.237
1.097TrpSer: 1.097 ± 0.304
0.499TrpThr: 0.499 ± 0.184
1.297TrpVal: 1.297 ± 0.344
0.299TrpTrp: 0.299 ± 0.205
0.599TrpTyr: 0.599 ± 0.231
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.794TyrAla: 2.794 ± 0.576
0.998TyrCys: 0.998 ± 0.28
1.995TyrAsp: 1.995 ± 0.507
2.893TyrGlu: 2.893 ± 0.539
1.297TyrPhe: 1.297 ± 0.379
2.295TyrGly: 2.295 ± 0.483
0.2TyrHis: 0.2 ± 0.142
1.896TyrIle: 1.896 ± 0.491
1.397TyrLys: 1.397 ± 0.411
2.394TyrLeu: 2.394 ± 0.405
1.097TyrMet: 1.097 ± 0.386
2.794TyrAsn: 2.794 ± 0.377
0.698TyrPro: 0.698 ± 0.278
1.497TyrGln: 1.497 ± 0.374
1.297TyrArg: 1.297 ± 0.402
2.893TyrSer: 2.893 ± 0.526
1.896TyrThr: 1.896 ± 0.455
2.095TyrVal: 2.095 ± 0.504
0.499TyrTrp: 0.499 ± 0.254
1.097TyrTyr: 1.097 ± 0.312
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (10024 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski