Streptococcus phage phiZJ20091101-2

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9I727|A0A1X9I727_9VIRU HicB-like domain-containing protein OS=Streptococcus phage phiZJ20091101-2 OX=1860191 PE=4 SV=1
MM1 pKa = 7.39LVTYY5 pKa = 8.27PALFYY10 pKa = 11.35YY11 pKa = 10.58DD12 pKa = 3.95DD13 pKa = 4.21TDD15 pKa = 3.79GATAPYY21 pKa = 9.93FVTFPDD27 pKa = 4.61FEE29 pKa = 5.3HH30 pKa = 7.07SATQGEE36 pKa = 4.5DD37 pKa = 3.09MADD40 pKa = 3.52AMAMASDD47 pKa = 3.34WLGIHH52 pKa = 6.56LADD55 pKa = 4.36YY56 pKa = 10.61IEE58 pKa = 4.46NGRR61 pKa = 11.84DD62 pKa = 3.14IPTPTPINALSLADD76 pKa = 3.93NNPFRR81 pKa = 11.84DD82 pKa = 3.87DD83 pKa = 3.58KK84 pKa = 11.4DD85 pKa = 3.43IEE87 pKa = 4.32LVYY90 pKa = 10.75DD91 pKa = 3.98PSKK94 pKa = 11.15SFVSMVMVDD103 pKa = 3.2VAEE106 pKa = 4.14YY107 pKa = 10.49LGSQEE112 pKa = 4.15PVKK115 pKa = 10.18KK116 pKa = 9.12TLTIPRR122 pKa = 11.84WADD125 pKa = 3.13TLGRR129 pKa = 11.84EE130 pKa = 4.01LGLNFSQTLTDD141 pKa = 5.45AIADD145 pKa = 4.03KK146 pKa = 10.56KK147 pKa = 10.31IHH149 pKa = 5.92AA150 pKa = 5.0

Molecular weight: 16.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9I5V5|A0A1X9I5V5_9VIRU Uncharacterized protein OS=Streptococcus phage phiZJ20091101-2 OX=1860191 PE=4 SV=1
MM1 pKa = 7.72IEE3 pKa = 4.18LTIEE7 pKa = 4.72SITKK11 pKa = 9.21PLKK14 pKa = 9.48QKK16 pKa = 10.39RR17 pKa = 11.84LSRR20 pKa = 11.84VSGKK24 pKa = 10.57LKK26 pKa = 10.78GVGITIDD33 pKa = 3.96LNNLTVNYY41 pKa = 9.96GSQQINLASIPGTYY55 pKa = 10.31GGVRR59 pKa = 11.84YY60 pKa = 9.44FFLCPICEE68 pKa = 4.06KK69 pKa = 10.5RR70 pKa = 11.84CRR72 pKa = 11.84KK73 pKa = 9.36LFKK76 pKa = 10.44NSLIFACGTCQDD88 pKa = 3.41RR89 pKa = 11.84HH90 pKa = 5.69RR91 pKa = 11.84ATLNRR96 pKa = 11.84SKK98 pKa = 9.84TDD100 pKa = 3.15CQYY103 pKa = 11.37YY104 pKa = 9.9FEE106 pKa = 5.27LAFKK110 pKa = 9.47EE111 pKa = 3.97ARR113 pKa = 11.84KK114 pKa = 9.38IDD116 pKa = 3.6PSYY119 pKa = 10.69YY120 pKa = 9.27PEE122 pKa = 5.38KK123 pKa = 10.98GYY125 pKa = 11.26VDD127 pKa = 3.77YY128 pKa = 11.42DD129 pKa = 3.72NFPSRR134 pKa = 11.84PKK136 pKa = 10.44RR137 pKa = 11.84MRR139 pKa = 11.84AKK141 pKa = 9.81TYY143 pKa = 7.21WKK145 pKa = 9.63HH146 pKa = 4.13YY147 pKa = 10.53RR148 pKa = 11.84KK149 pKa = 8.53FTSYY153 pKa = 11.04LDD155 pKa = 3.52KK156 pKa = 11.23GVSNWLGGLKK166 pKa = 10.28

Molecular weight: 19.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19

0

19

2884

52

435

151.8

17.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.039 ± 0.729

0.624 ± 0.221

6.345 ± 0.465

7.836 ± 0.812

3.537 ± 0.299

5.548 ± 0.801

1.491 ± 0.205

6.761 ± 0.523

8.911 ± 0.701

9.258 ± 0.641

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.462 ± 0.197

5.028 ± 0.505

3.155 ± 0.422

4.334 ± 0.576

4.646 ± 0.45

5.374 ± 0.309

6.449 ± 0.309

5.791 ± 0.522

1.075 ± 0.196

4.334 ± 0.449
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski