Amino acid dipepetide frequency for Mycobacterium phage Aggie

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.958AlaAla: 19.958 ± 2.542
0.7AlaCys: 0.7 ± 0.276
9.104AlaAsp: 9.104 ± 0.684
8.543AlaGlu: 8.543 ± 1.288
3.151AlaPhe: 3.151 ± 0.504
10.014AlaGly: 10.014 ± 1.457
2.241AlaHis: 2.241 ± 0.467
5.462AlaIle: 5.462 ± 0.561
3.852AlaLys: 3.852 ± 0.649
9.524AlaLeu: 9.524 ± 0.861
1.961AlaMet: 1.961 ± 0.391
3.571AlaAsn: 3.571 ± 0.615
7.073AlaPro: 7.073 ± 0.747
5.532AlaGln: 5.532 ± 0.683
7.633AlaArg: 7.633 ± 0.928
6.303AlaSer: 6.303 ± 0.68
7.913AlaThr: 7.913 ± 0.873
7.423AlaVal: 7.423 ± 0.651
1.961AlaTrp: 1.961 ± 0.44
2.381AlaTyr: 2.381 ± 0.449
0.0AlaXaa: 0.0 ± 0.0
Cys
1.401CysAla: 1.401 ± 0.346
0.21CysCys: 0.21 ± 0.122
1.261CysAsp: 1.261 ± 0.319
0.49CysGlu: 0.49 ± 0.201
0.07CysPhe: 0.07 ± 0.07
1.261CysGly: 1.261 ± 0.355
0.21CysHis: 0.21 ± 0.139
0.07CysIle: 0.07 ± 0.076
0.14CysLys: 0.14 ± 0.089
0.7CysLeu: 0.7 ± 0.23
0.21CysMet: 0.21 ± 0.124
0.35CysAsn: 0.35 ± 0.188
1.331CysPro: 1.331 ± 0.389
0.7CysGln: 0.7 ± 0.21
0.77CysArg: 0.77 ± 0.254
0.49CysSer: 0.49 ± 0.176
0.63CysThr: 0.63 ± 0.189
0.77CysVal: 0.77 ± 0.238
0.14CysTrp: 0.14 ± 0.116
0.14CysTyr: 0.14 ± 0.097
0.0CysXaa: 0.0 ± 0.0
Asp
7.563AspAla: 7.563 ± 0.746
1.12AspCys: 1.12 ± 0.357
5.462AspAsp: 5.462 ± 0.83
4.832AspGlu: 4.832 ± 0.756
1.751AspPhe: 1.751 ± 0.347
6.443AspGly: 6.443 ± 0.727
1.471AspHis: 1.471 ± 0.379
3.011AspIle: 3.011 ± 0.401
1.471AspLys: 1.471 ± 0.347
6.022AspLeu: 6.022 ± 0.646
1.471AspMet: 1.471 ± 0.265
1.821AspAsn: 1.821 ± 0.413
4.342AspPro: 4.342 ± 0.534
2.381AspGln: 2.381 ± 0.517
3.922AspArg: 3.922 ± 0.657
3.992AspSer: 3.992 ± 0.59
3.501AspThr: 3.501 ± 0.475
3.291AspVal: 3.291 ± 0.419
1.19AspTrp: 1.19 ± 0.3
1.261AspTyr: 1.261 ± 0.304
0.0AspXaa: 0.0 ± 0.0
Glu
5.812GluAla: 5.812 ± 0.818
0.84GluCys: 0.84 ± 0.249
3.571GluAsp: 3.571 ± 0.483
1.541GluGlu: 1.541 ± 0.388
2.521GluPhe: 2.521 ± 0.547
3.221GluGly: 3.221 ± 0.563
1.331GluHis: 1.331 ± 0.306
2.661GluIle: 2.661 ± 0.48
1.961GluLys: 1.961 ± 0.371
5.252GluLeu: 5.252 ± 0.632
1.961GluMet: 1.961 ± 0.459
1.541GluAsn: 1.541 ± 0.289
3.291GluPro: 3.291 ± 0.478
3.011GluGln: 3.011 ± 0.466
4.062GluArg: 4.062 ± 0.805
3.501GluSer: 3.501 ± 0.628
3.782GluThr: 3.782 ± 0.666
3.992GluVal: 3.992 ± 0.568
1.05GluTrp: 1.05 ± 0.327
1.19GluTyr: 1.19 ± 0.268
0.0GluXaa: 0.0 ± 0.0
Phe
3.291PheAla: 3.291 ± 0.529
0.49PheCys: 0.49 ± 0.217
1.261PheAsp: 1.261 ± 0.342
1.611PheGlu: 1.611 ± 0.411
1.05PhePhe: 1.05 ± 0.304
3.501PheGly: 3.501 ± 0.489
0.84PheHis: 0.84 ± 0.276
1.331PheIle: 1.331 ± 0.265
0.77PheLys: 0.77 ± 0.2
1.681PheLeu: 1.681 ± 0.35
0.63PheMet: 0.63 ± 0.227
0.91PheAsn: 0.91 ± 0.261
1.05PhePro: 1.05 ± 0.286
0.56PheGln: 0.56 ± 0.188
1.681PheArg: 1.681 ± 0.27
1.541PheSer: 1.541 ± 0.356
2.171PheThr: 2.171 ± 0.364
2.591PheVal: 2.591 ± 0.482
0.21PheTrp: 0.21 ± 0.149
1.12PheTyr: 1.12 ± 0.317
0.0PheXaa: 0.0 ± 0.0
Gly
8.824GlyAla: 8.824 ± 1.362
0.7GlyCys: 0.7 ± 0.243
5.742GlyAsp: 5.742 ± 0.627
3.922GlyGlu: 3.922 ± 0.575
2.801GlyPhe: 2.801 ± 0.505
9.944GlyGly: 9.944 ± 1.494
1.681GlyHis: 1.681 ± 0.311
4.482GlyIle: 4.482 ± 0.769
2.241GlyLys: 2.241 ± 0.346
6.583GlyLeu: 6.583 ± 0.775
1.541GlyMet: 1.541 ± 0.342
2.941GlyAsn: 2.941 ± 0.634
4.692GlyPro: 4.692 ± 0.66
4.272GlyGln: 4.272 ± 0.551
5.952GlyArg: 5.952 ± 0.585
4.692GlySer: 4.692 ± 0.731
5.952GlyThr: 5.952 ± 0.801
6.583GlyVal: 6.583 ± 0.731
2.241GlyTrp: 2.241 ± 0.343
3.011GlyTyr: 3.011 ± 0.668
0.0GlyXaa: 0.0 ± 0.0
His
1.611HisAla: 1.611 ± 0.367
0.42HisCys: 0.42 ± 0.191
1.331HisAsp: 1.331 ± 0.349
1.401HisGlu: 1.401 ± 0.321
0.21HisPhe: 0.21 ± 0.13
1.401HisGly: 1.401 ± 0.303
0.98HisHis: 0.98 ± 0.279
1.05HisIle: 1.05 ± 0.238
0.49HisLys: 0.49 ± 0.178
1.471HisLeu: 1.471 ± 0.502
0.42HisMet: 0.42 ± 0.177
0.35HisAsn: 0.35 ± 0.17
1.541HisPro: 1.541 ± 0.418
0.98HisGln: 0.98 ± 0.314
2.031HisArg: 2.031 ± 0.402
0.42HisSer: 0.42 ± 0.2
1.751HisThr: 1.751 ± 0.373
1.05HisVal: 1.05 ± 0.247
0.49HisTrp: 0.49 ± 0.169
0.91HisTyr: 0.91 ± 0.246
0.0HisXaa: 0.0 ± 0.0
Ile
5.882IleAla: 5.882 ± 0.763
0.21IleCys: 0.21 ± 0.122
3.361IleAsp: 3.361 ± 0.5
4.482IleGlu: 4.482 ± 0.48
0.77IlePhe: 0.77 ± 0.236
4.412IleGly: 4.412 ± 0.502
1.12IleHis: 1.12 ± 0.334
1.331IleIle: 1.331 ± 0.303
0.91IleLys: 0.91 ± 0.325
2.101IleLeu: 2.101 ± 0.405
0.21IleMet: 0.21 ± 0.114
2.031IleAsn: 2.031 ± 0.396
2.661IlePro: 2.661 ± 0.479
1.331IleGln: 1.331 ± 0.324
3.782IleArg: 3.782 ± 0.496
2.171IleSer: 2.171 ± 0.369
3.711IleThr: 3.711 ± 0.466
2.661IleVal: 2.661 ± 0.509
0.91IleTrp: 0.91 ± 0.234
0.77IleTyr: 0.77 ± 0.202
0.0IleXaa: 0.0 ± 0.0
Lys
3.711LysAla: 3.711 ± 0.956
0.42LysCys: 0.42 ± 0.234
1.05LysAsp: 1.05 ± 0.262
1.19LysGlu: 1.19 ± 0.228
0.91LysPhe: 0.91 ± 0.229
2.311LysGly: 2.311 ± 0.404
0.35LysHis: 0.35 ± 0.157
1.331LysIle: 1.331 ± 0.282
0.56LysLys: 0.56 ± 0.158
2.381LysLeu: 2.381 ± 0.529
0.63LysMet: 0.63 ± 0.212
0.49LysAsn: 0.49 ± 0.189
2.521LysPro: 2.521 ± 0.439
1.261LysGln: 1.261 ± 0.338
2.381LysArg: 2.381 ± 0.412
1.821LysSer: 1.821 ± 0.395
1.681LysThr: 1.681 ± 0.351
2.241LysVal: 2.241 ± 0.362
0.63LysTrp: 0.63 ± 0.188
0.84LysTyr: 0.84 ± 0.234
0.0LysXaa: 0.0 ± 0.0
Leu
10.644LeuAla: 10.644 ± 1.041
0.77LeuCys: 0.77 ± 0.24
5.532LeuAsp: 5.532 ± 0.665
2.941LeuGlu: 2.941 ± 0.407
2.521LeuPhe: 2.521 ± 0.469
8.053LeuGly: 8.053 ± 1.087
1.261LeuHis: 1.261 ± 0.336
3.291LeuIle: 3.291 ± 0.354
2.591LeuLys: 2.591 ± 0.471
7.213LeuLeu: 7.213 ± 0.752
1.05LeuMet: 1.05 ± 0.298
2.381LeuAsn: 2.381 ± 0.506
4.482LeuPro: 4.482 ± 0.811
2.521LeuGln: 2.521 ± 0.421
5.602LeuArg: 5.602 ± 0.9
4.132LeuSer: 4.132 ± 0.556
5.672LeuThr: 5.672 ± 0.738
5.112LeuVal: 5.112 ± 0.503
1.261LeuTrp: 1.261 ± 0.283
1.19LeuTyr: 1.19 ± 0.293
0.0LeuXaa: 0.0 ± 0.0
Met
3.081MetAla: 3.081 ± 0.387
0.14MetCys: 0.14 ± 0.145
0.49MetAsp: 0.49 ± 0.185
0.91MetGlu: 0.91 ± 0.323
0.98MetPhe: 0.98 ± 0.261
0.63MetGly: 0.63 ± 0.21
0.21MetHis: 0.21 ± 0.118
0.98MetIle: 0.98 ± 0.274
0.63MetLys: 0.63 ± 0.221
1.19MetLeu: 1.19 ± 0.273
0.56MetMet: 0.56 ± 0.176
0.77MetAsn: 0.77 ± 0.284
1.331MetPro: 1.331 ± 0.313
0.77MetGln: 0.77 ± 0.234
1.261MetArg: 1.261 ± 0.347
2.661MetSer: 2.661 ± 0.512
1.821MetThr: 1.821 ± 0.305
0.84MetVal: 0.84 ± 0.203
0.77MetTrp: 0.77 ± 0.301
0.35MetTyr: 0.35 ± 0.16
0.0MetXaa: 0.0 ± 0.0
Asn
3.291AsnAla: 3.291 ± 0.556
0.42AsnCys: 0.42 ± 0.152
1.611AsnAsp: 1.611 ± 0.329
0.84AsnGlu: 0.84 ± 0.26
0.91AsnPhe: 0.91 ± 0.218
3.782AsnGly: 3.782 ± 0.693
0.28AsnHis: 0.28 ± 0.204
1.611AsnIle: 1.611 ± 0.436
0.77AsnLys: 0.77 ± 0.324
2.941AsnLeu: 2.941 ± 0.469
0.42AsnMet: 0.42 ± 0.163
1.261AsnAsn: 1.261 ± 0.345
2.941AsnPro: 2.941 ± 0.397
1.541AsnGln: 1.541 ± 0.363
2.031AsnArg: 2.031 ± 0.364
1.541AsnSer: 1.541 ± 0.332
1.821AsnThr: 1.821 ± 0.33
1.681AsnVal: 1.681 ± 0.422
0.49AsnTrp: 0.49 ± 0.174
0.63AsnTyr: 0.63 ± 0.224
0.0AsnXaa: 0.0 ± 0.0
Pro
7.493ProAla: 7.493 ± 0.915
0.49ProCys: 0.49 ± 0.182
4.762ProAsp: 4.762 ± 0.505
4.832ProGlu: 4.832 ± 0.734
1.681ProPhe: 1.681 ± 0.31
6.373ProGly: 6.373 ± 0.871
1.401ProHis: 1.401 ± 0.314
2.591ProIle: 2.591 ± 0.415
1.331ProLys: 1.331 ± 0.294
3.992ProLeu: 3.992 ± 0.476
1.471ProMet: 1.471 ± 0.397
2.031ProAsn: 2.031 ± 0.378
3.992ProPro: 3.992 ± 0.513
2.101ProGln: 2.101 ± 0.383
3.221ProArg: 3.221 ± 0.614
3.431ProSer: 3.431 ± 0.533
3.922ProThr: 3.922 ± 0.504
4.552ProVal: 4.552 ± 0.502
1.611ProTrp: 1.611 ± 0.375
1.261ProTyr: 1.261 ± 0.372
0.0ProXaa: 0.0 ± 0.0
Gln
5.252GlnAla: 5.252 ± 0.883
0.35GlnCys: 0.35 ± 0.183
1.751GlnAsp: 1.751 ± 0.521
1.471GlnGlu: 1.471 ± 0.445
1.261GlnPhe: 1.261 ± 0.265
2.171GlnGly: 2.171 ± 0.336
1.05GlnHis: 1.05 ± 0.299
2.801GlnIle: 2.801 ± 0.522
1.12GlnLys: 1.12 ± 0.315
4.342GlnLeu: 4.342 ± 0.525
0.91GlnMet: 0.91 ± 0.241
0.7GlnAsn: 0.7 ± 0.234
2.451GlnPro: 2.451 ± 0.441
1.471GlnGln: 1.471 ± 0.461
3.782GlnArg: 3.782 ± 0.749
1.541GlnSer: 1.541 ± 0.392
2.521GlnThr: 2.521 ± 0.378
3.081GlnVal: 3.081 ± 0.52
0.56GlnTrp: 0.56 ± 0.168
0.84GlnTyr: 0.84 ± 0.247
0.0GlnXaa: 0.0 ± 0.0
Arg
7.213ArgAla: 7.213 ± 0.794
1.05ArgCys: 1.05 ± 0.348
4.762ArgAsp: 4.762 ± 0.542
4.202ArgGlu: 4.202 ± 0.831
1.821ArgPhe: 1.821 ± 0.482
4.972ArgGly: 4.972 ± 0.537
1.681ArgHis: 1.681 ± 0.375
3.571ArgIle: 3.571 ± 0.581
2.451ArgLys: 2.451 ± 0.515
4.692ArgLeu: 4.692 ± 0.597
1.961ArgMet: 1.961 ± 0.525
2.241ArgAsn: 2.241 ± 0.427
4.272ArgPro: 4.272 ± 0.543
3.221ArgGln: 3.221 ± 0.818
6.022ArgArg: 6.022 ± 1.036
2.311ArgSer: 2.311 ± 0.443
4.272ArgThr: 4.272 ± 0.696
4.832ArgVal: 4.832 ± 0.661
1.611ArgTrp: 1.611 ± 0.35
1.611ArgTyr: 1.611 ± 0.382
0.0ArgXaa: 0.0 ± 0.0
Ser
7.633SerAla: 7.633 ± 1.006
0.42SerCys: 0.42 ± 0.184
3.291SerAsp: 3.291 ± 0.578
1.961SerGlu: 1.961 ± 0.398
1.541SerPhe: 1.541 ± 0.335
5.602SerGly: 5.602 ± 0.737
0.56SerHis: 0.56 ± 0.237
1.681SerIle: 1.681 ± 0.354
1.681SerLys: 1.681 ± 0.33
3.922SerLeu: 3.922 ± 0.532
2.031SerMet: 2.031 ± 0.358
1.261SerAsn: 1.261 ± 0.335
3.361SerPro: 3.361 ± 0.411
1.891SerGln: 1.891 ± 0.429
3.081SerArg: 3.081 ± 0.465
3.081SerSer: 3.081 ± 0.497
3.221SerThr: 3.221 ± 0.416
3.501SerVal: 3.501 ± 0.443
1.05SerTrp: 1.05 ± 0.252
1.681SerTyr: 1.681 ± 0.338
0.0SerXaa: 0.0 ± 0.0
Thr
8.894ThrAla: 8.894 ± 0.824
0.56ThrCys: 0.56 ± 0.211
4.342ThrAsp: 4.342 ± 0.547
3.641ThrGlu: 3.641 ± 0.456
1.681ThrPhe: 1.681 ± 0.36
6.513ThrGly: 6.513 ± 0.852
0.84ThrHis: 0.84 ± 0.213
2.801ThrIle: 2.801 ± 0.37
1.891ThrLys: 1.891 ± 0.419
5.742ThrLeu: 5.742 ± 0.647
1.261ThrMet: 1.261 ± 0.271
1.821ThrAsn: 1.821 ± 0.325
5.392ThrPro: 5.392 ± 0.682
2.241ThrGln: 2.241 ± 0.361
3.852ThrArg: 3.852 ± 0.643
2.871ThrSer: 2.871 ± 0.505
5.112ThrThr: 5.112 ± 0.844
6.723ThrVal: 6.723 ± 1.017
0.77ThrTrp: 0.77 ± 0.235
1.05ThrTyr: 1.05 ± 0.259
0.0ThrXaa: 0.0 ± 0.0
Val
9.174ValAla: 9.174 ± 0.861
0.84ValCys: 0.84 ± 0.221
4.552ValAsp: 4.552 ± 0.592
5.322ValGlu: 5.322 ± 0.703
1.611ValPhe: 1.611 ± 0.38
5.182ValGly: 5.182 ± 0.682
1.331ValHis: 1.331 ± 0.314
3.151ValIle: 3.151 ± 0.598
2.871ValLys: 2.871 ± 0.556
4.972ValLeu: 4.972 ± 0.588
0.98ValMet: 0.98 ± 0.294
2.871ValAsn: 2.871 ± 0.481
3.641ValPro: 3.641 ± 0.468
2.171ValGln: 2.171 ± 0.385
5.112ValArg: 5.112 ± 0.635
3.431ValSer: 3.431 ± 0.426
5.252ValThr: 5.252 ± 0.595
4.552ValVal: 4.552 ± 0.643
0.77ValTrp: 0.77 ± 0.231
1.19ValTyr: 1.19 ± 0.271
0.0ValXaa: 0.0 ± 0.0
Trp
1.12TrpAla: 1.12 ± 0.3
0.63TrpCys: 0.63 ± 0.281
0.98TrpAsp: 0.98 ± 0.232
0.49TrpGlu: 0.49 ± 0.177
0.42TrpPhe: 0.42 ± 0.18
0.98TrpGly: 0.98 ± 0.244
0.63TrpHis: 0.63 ± 0.206
0.91TrpIle: 0.91 ± 0.251
0.28TrpLys: 0.28 ± 0.147
1.961TrpLeu: 1.961 ± 0.416
0.28TrpMet: 0.28 ± 0.154
0.7TrpAsn: 0.7 ± 0.212
0.98TrpPro: 0.98 ± 0.251
0.91TrpGln: 0.91 ± 0.217
1.401TrpArg: 1.401 ± 0.304
1.401TrpSer: 1.401 ± 0.27
1.891TrpThr: 1.891 ± 0.289
1.751TrpVal: 1.751 ± 0.314
0.7TrpTrp: 0.7 ± 0.197
0.49TrpTyr: 0.49 ± 0.196
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.451TyrAla: 2.451 ± 0.4
0.56TyrCys: 0.56 ± 0.201
2.101TyrAsp: 2.101 ± 0.375
1.471TyrGlu: 1.471 ± 0.254
0.63TyrPhe: 0.63 ± 0.22
2.031TyrGly: 2.031 ± 0.34
0.84TyrHis: 0.84 ± 0.229
0.7TyrIle: 0.7 ± 0.242
0.7TyrLys: 0.7 ± 0.232
1.541TyrLeu: 1.541 ± 0.298
0.35TyrMet: 0.35 ± 0.142
0.77TyrAsn: 0.77 ± 0.221
1.19TyrPro: 1.19 ± 0.342
0.56TyrGln: 0.56 ± 0.171
1.261TyrArg: 1.261 ± 0.334
1.12TyrSer: 1.12 ± 0.264
1.401TyrThr: 1.401 ± 0.34
1.751TyrVal: 1.751 ± 0.419
0.49TyrTrp: 0.49 ± 0.243
0.63TyrTyr: 0.63 ± 0.227
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (14281 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski