Novosphingobium sp. PhB165

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium; unclassified Novosphingobium

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5096 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R1UXJ1|A0A4R1UXJ1_9SPHN Putative redox protein OS=Novosphingobium sp. PhB165 OX=2485105 GN=EDF56_101364 PE=4 SV=1
MM1 pKa = 7.01TARR4 pKa = 11.84LFALFATLAALLLAGTAQAQNATAAWSSLGLSNGGSVGSSTAITASDD51 pKa = 3.58GTTVSTAWSTTVGGAGTFAVASGYY75 pKa = 10.82NNYY78 pKa = 9.38VVYY81 pKa = 8.73NTPAIGGVSPGLLLNFDD98 pKa = 4.42ASRR101 pKa = 11.84FDD103 pKa = 4.05ANNKK107 pKa = 7.0ITMDD111 pKa = 2.9ITLNRR116 pKa = 11.84SVTGLAFSIADD127 pKa = 3.41IDD129 pKa = 5.16RR130 pKa = 11.84EE131 pKa = 4.65TTGNNYY137 pKa = 9.48NRR139 pKa = 11.84DD140 pKa = 3.77AVAVYY145 pKa = 10.56YY146 pKa = 8.61DD147 pKa = 3.62TGNGTFVNAVSNSAFWSDD165 pKa = 3.41GSAVTNDD172 pKa = 3.04TSGWNGTAASANNSTNGTLTFNFGTTAVKK201 pKa = 10.35RR202 pKa = 11.84IRR204 pKa = 11.84IVYY207 pKa = 9.84NSYY210 pKa = 8.74TVSGTANPTAQFIVLSGLAFNAKK233 pKa = 9.99GADD236 pKa = 3.44LSLGNTLLTSSPTSGGLATFRR257 pKa = 11.84LTVTNASTSTDD268 pKa = 2.92TATDD272 pKa = 3.45VKK274 pKa = 10.64VTDD277 pKa = 3.83TLPDD281 pKa = 3.14GFTYY285 pKa = 10.62VSSNGTGSFNAATGVWTVGSIAPGASVTLDD315 pKa = 2.86ITGTVNASAGATLTNTAEE333 pKa = 4.23ITASDD338 pKa = 3.86QNDD341 pKa = 3.82PDD343 pKa = 3.98STPGNGVTSEE353 pKa = 4.66DD354 pKa = 4.48DD355 pKa = 3.63YY356 pKa = 12.26ASATLTVTGSRR367 pKa = 11.84VAGTPPTLSCAAGTVVFDD385 pKa = 3.63WSNRR389 pKa = 11.84AWTAGSTSNSYY400 pKa = 11.31SFDD403 pKa = 3.47TLGTISFALANQGTWLSSSTYY424 pKa = 10.15GGQSPVRR431 pKa = 11.84QNVVNGGLNDD441 pKa = 3.5YY442 pKa = 11.49ALFEE446 pKa = 4.44MVNMPNISAEE456 pKa = 4.04ATTTITLPTALPGAQFKK473 pKa = 10.87IFDD476 pKa = 3.34VDD478 pKa = 3.9YY479 pKa = 11.34YY480 pKa = 11.29SGQFADD486 pKa = 3.57KK487 pKa = 9.14VTVEE491 pKa = 3.79GRR493 pKa = 11.84YY494 pKa = 9.74NGAVVTPTLTNGVSNYY510 pKa = 10.32VIGNSAYY517 pKa = 10.51GDD519 pKa = 4.04GLSDD523 pKa = 4.49DD524 pKa = 4.55ASADD528 pKa = 3.39GTVTVTFSQPIDD540 pKa = 3.78TIIVRR545 pKa = 11.84YY546 pKa = 9.09GNHH549 pKa = 5.69SMAPSDD555 pKa = 4.23PGQQAIALHH564 pKa = 7.4DD565 pKa = 3.4ITFCRR570 pKa = 11.84PSTTLAVSKK579 pKa = 9.53TSSVVSDD586 pKa = 3.89PVSGTNNPKK595 pKa = 10.34AIPGATVRR603 pKa = 11.84YY604 pKa = 8.83CILVANTGSSAATSVAASDD623 pKa = 5.24PIPATLTYY631 pKa = 10.2VAGSIYY637 pKa = 10.53SGSDD641 pKa = 3.11CASANTAEE649 pKa = 5.09DD650 pKa = 4.52DD651 pKa = 3.7NDD653 pKa = 4.12TGSDD657 pKa = 3.32EE658 pKa = 4.29SDD660 pKa = 3.48PYY662 pKa = 9.82GASYY666 pKa = 11.5ASGTVKK672 pKa = 10.67ASAPTLAAGTSFAIRR687 pKa = 11.84FDD689 pKa = 3.42ATVNN693 pKa = 3.43

Molecular weight: 70.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R1UQE9|A0A4R1UQE9_9SPHN Two-component system chemotaxis response regulator CheY OS=Novosphingobium sp. PhB165 OX=2485105 GN=EDF56_103211 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.54GFRR19 pKa = 11.84ARR21 pKa = 11.84TATVGGRR28 pKa = 11.84KK29 pKa = 8.17VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5096

0

5096

1697015

29

6018

333.0

35.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.998 ± 0.052

0.823 ± 0.011

5.822 ± 0.026

5.447 ± 0.041

3.566 ± 0.021

9.074 ± 0.084

2.072 ± 0.017

4.759 ± 0.022

2.941 ± 0.026

9.779 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.432 ± 0.021

2.684 ± 0.027

5.375 ± 0.028

3.173 ± 0.019

6.948 ± 0.051

5.589 ± 0.034

5.49 ± 0.05

7.271 ± 0.027

1.476 ± 0.015

2.282 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski