Neochlamydia sp. TUME1

Taxonomy: cellular organisms; Bacteria; PVC group; Chlamydiae; Chlamydiia; Parachlamydiales; Parachlamydiaceae; Neochlamydia; unclassified Neochlamydia

Average proteome isoelectric point is 7.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2344 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C1JL86|A0A0C1JL86_9BACT zf-IS66 domain-containing protein OS=Neochlamydia sp. TUME1 OX=1478174 GN=DB41_BK00010 PE=4 SV=1
DDD2 pKa = 4.76IAEEE6 pKa = 3.87RR7 pKa = 11.84KKK9 pKa = 10.11ITALDDD15 pKa = 4.18SRR17 pKa = 11.84IDDD20 pKa = 3.86TYYY23 pKa = 10.64PPEEE27 pKa = 4.3CQLSQLQTLDDD38 pKa = 3.43RR39 pKa = 11.84EEE41 pKa = 4.2QLTALPTEEE50 pKa = 4.7GQLSEEE56 pKa = 4.32QYYY59 pKa = 11.42YYY61 pKa = 11.24NQNQLTALPAEEE73 pKa = 4.49GRR75 pKa = 11.84LSEEE79 pKa = 4.12QSLEEE84 pKa = 4.53NQNQLTSLPAEEE96 pKa = 4.4GQLSKKK102 pKa = 11.21QVLHHH107 pKa = 7.09SQNQLTALPAEEE119 pKa = 4.63GQLSEEE125 pKa = 4.45QALDDD130 pKa = 4.36SQNQLTALPAEEE142 pKa = 4.63GQLSEEE148 pKa = 5.62KKK150 pKa = 9.97LDDD153 pKa = 3.74RR154 pKa = 11.84EEE156 pKa = 4.31EEE158 pKa = 4.51NNLPAEEE165 pKa = 4.46GRR167 pKa = 11.84LPQLQWLDDD176 pKa = 3.47SQNQLVSLPEEE187 pKa = 4.56IGRR190 pKa = 11.84LSQLQEEE197 pKa = 4.18NSSQNQLTNLPEEE210 pKa = 4.7EE211 pKa = 4.7GQLPQLQWLYYY222 pKa = 11.42NQNQLTALPSEEE234 pKa = 4.15RR235 pKa = 11.84QLFQLRR241 pKa = 11.84GLYYY245 pKa = 10.54NQNQLTSLPEEE256 pKa = 4.25EE257 pKa = 4.47GQLSQLQWLYYY268 pKa = 11.53NQNQLTSLPAEEE280 pKa = 4.62GQLPQLQEEE289 pKa = 4.18EEE291 pKa = 4.65NQNQLTSLPAEEE303 pKa = 4.54GQLSQLQVLYYY314 pKa = 11.07NQNQLASLPAEEE326 pKa = 4.5GQLPQLQEEE335 pKa = 4.35EEE337 pKa = 4.4SQNQLTSLPAEEE349 pKa = 4.4GQLSKKK355 pKa = 11.1QEEE358 pKa = 4.22YYY360 pKa = 11.14NQNQLTSLPVEEE372 pKa = 4.67GQLSQLQGFEEE383 pKa = 4.35SQNQLTALPVEEE395 pKa = 4.92GQLSQLQGLYYY406 pKa = 10.79NQNQLTSLPAEEE418 pKa = 4.54GQLSQLQEEE427 pKa = 4.04EEE429 pKa = 4.6NQNQLTSLPVDDD441 pKa = 3.53GQLSQLRR448 pKa = 11.84RR449 pKa = 11.84LEEE452 pKa = 4.49NHHH455 pKa = 6.22QLTALPTEEE464 pKa = 4.85GQLSQLQGLYYY475 pKa = 10.79NQNQLTSLPIEEE487 pKa = 4.61GQLSPLLQLEEE498 pKa = 4.29AE

Molecular weight: 56.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C1HST5|A0A0C1HST5_9BACT Leucine-rich repeat-containing protein 1 OS=Neochlamydia sp. TUME1 OX=1478174 GN=lrrC1_1 PE=4 SV=1
MM1 pKa = 7.83IGRR4 pKa = 11.84KK5 pKa = 8.61LAYY8 pKa = 9.55HH9 pKa = 6.37KK10 pKa = 10.44HH11 pKa = 5.64HH12 pKa = 5.61LHH14 pKa = 6.72KK15 pKa = 10.73RR16 pKa = 11.84FLCSLVAKK24 pKa = 9.93RR25 pKa = 11.84SSGSLFNHH33 pKa = 6.44RR34 pKa = 11.84LDD36 pKa = 4.21YY37 pKa = 11.14SLSPYY42 pKa = 8.26LTKK45 pKa = 10.71SHH47 pKa = 6.82ASLGKK52 pKa = 9.6YY53 pKa = 10.0LSEE56 pKa = 4.2SFSKK60 pKa = 10.39FFVWLCHH67 pKa = 6.2ADD69 pKa = 3.71ALATLPHH76 pKa = 7.1PKK78 pKa = 9.94RR79 pKa = 11.84LLQRR83 pKa = 11.84NQRR86 pKa = 11.84LCLLDD91 pKa = 3.74ALRR94 pKa = 11.84LSFLISSCPSSLARR108 pKa = 11.84LQMRR112 pKa = 11.84VV113 pKa = 2.94

Molecular weight: 13.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2344

0

2344

675547

36

2477

288.2

32.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.218 ± 0.045

1.245 ± 0.019

4.08 ± 0.035

6.975 ± 0.056

4.413 ± 0.043

5.475 ± 0.055

2.566 ± 0.028

7.232 ± 0.047

7.395 ± 0.056

12.243 ± 0.099

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.105 ± 0.027

4.727 ± 0.041

4.156 ± 0.037

5.017 ± 0.057

4.278 ± 0.038

6.669 ± 0.046

4.697 ± 0.037

4.873 ± 0.052

1.106 ± 0.019

3.53 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski