Nitrospira sp. SCGC AG-212-E16

Taxonomy: cellular organisms; Bacteria; Nitrospirae; Nitrospira; Nitrospirales; Nitrospiraceae; Nitrospira; unclassified Nitrospira

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2093 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177QQH9|A0A177QQH9_9BACT Haemagg_act domain-containing protein OS=Nitrospira sp. SCGC AG-212-E16 OX=1799664 GN=AYO43_00010 PE=4 SV=1
MM1 pKa = 7.17GVVAEE6 pKa = 4.57GVVLLAVSAVACVACPSAMPDD27 pKa = 3.14TEE29 pKa = 4.22AAGGVWDD36 pKa = 4.32GVGGAVIVAMGGRR49 pKa = 11.84LVNVRR54 pKa = 11.84CCGVGIGAGDD64 pKa = 3.54IDD66 pKa = 4.59NEE68 pKa = 4.42GLCGSIGLAMCVGSVPEE85 pKa = 4.25GG86 pKa = 3.26

Molecular weight: 8.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177Q9V0|A0A177Q9V0_9BACT Uncharacterized protein OS=Nitrospira sp. SCGC AG-212-E16 OX=1799664 GN=AYO43_09030 PE=4 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84LFALSIRR9 pKa = 11.84PLWHH13 pKa = 6.14GVCVPAGIMAQCHH26 pKa = 4.35TTFVALIAVSLILIAPHH43 pKa = 6.3QISAQQSTAPPSSLSTRR60 pKa = 11.84PLAPGTTEE68 pKa = 3.76SEE70 pKa = 4.18LRR72 pKa = 11.84PSCDD76 pKa = 2.84LCRR79 pKa = 11.84KK80 pKa = 8.88PEE82 pKa = 3.93SRR84 pKa = 11.84AGNTLNPHH92 pKa = 5.94RR93 pKa = 11.84LHH95 pKa = 6.83RR96 pKa = 11.84EE97 pKa = 3.92KK98 pKa = 10.86GLHH101 pKa = 5.03PHH103 pKa = 7.02KK104 pKa = 10.14KK105 pKa = 7.57PKK107 pKa = 10.01GMRR110 pKa = 11.84RR111 pKa = 11.84SSRR114 pKa = 11.84RR115 pKa = 11.84SVRR118 pKa = 11.84SLLRR122 pKa = 11.84RR123 pKa = 11.84QAFAPLGGIEE133 pKa = 4.04RR134 pKa = 11.84TVDD137 pKa = 2.88SRR139 pKa = 11.84QVHH142 pKa = 5.4VVDD145 pKa = 4.73GDD147 pKa = 3.62TFRR150 pKa = 11.84YY151 pKa = 7.28GTEE154 pKa = 3.73RR155 pKa = 11.84VRR157 pKa = 11.84LRR159 pKa = 11.84GIDD162 pKa = 3.62TPEE165 pKa = 4.42LDD167 pKa = 4.21EE168 pKa = 5.49PNGQAARR175 pKa = 11.84LRR177 pKa = 11.84LEE179 pKa = 4.28EE180 pKa = 4.73LLHH183 pKa = 6.02SGQVRR188 pKa = 11.84IVPHH192 pKa = 6.1GRR194 pKa = 11.84DD195 pKa = 3.26VYY197 pKa = 11.34DD198 pKa = 3.84RR199 pKa = 11.84LVADD203 pKa = 3.65VFVDD207 pKa = 3.68GRR209 pKa = 11.84NVTDD213 pKa = 3.62MLTQEE218 pKa = 5.62GYY220 pKa = 11.11AKK222 pKa = 10.23PRR224 pKa = 11.84SS225 pKa = 3.66

Molecular weight: 25.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2093

0

2093

575471

37

1567

275.0

30.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.556 ± 0.057

1.015 ± 0.022

5.097 ± 0.035

6.047 ± 0.047

3.657 ± 0.034

7.8 ± 0.056

2.401 ± 0.031

5.363 ± 0.045

4.358 ± 0.057

10.474 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.477 ± 0.027

2.724 ± 0.034

5.009 ± 0.039

4.015 ± 0.035

6.653 ± 0.06

5.897 ± 0.049

5.819 ± 0.042

7.688 ± 0.053

1.367 ± 0.023

2.583 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski