Rat associated porprismacovirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Porprismacovirus

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1R815|A0A0K1R815_9VIRU Rep protein OS=Rat associated porprismacovirus 1 OX=2170126 PE=4 SV=1
MM1 pKa = 7.11VSASISEE8 pKa = 4.34IYY10 pKa = 10.45DD11 pKa = 3.34LSTTVGQGTVLKK23 pKa = 10.43IHH25 pKa = 6.62TPTGNNVRR33 pKa = 11.84RR34 pKa = 11.84HH35 pKa = 4.83LLGYY39 pKa = 9.1FLQYY43 pKa = 10.96KK44 pKa = 9.06KK45 pKa = 10.68FRR47 pKa = 11.84YY48 pKa = 9.41VGAKK52 pKa = 7.92VTLVPASTLPADD64 pKa = 4.26PLQLSYY70 pKa = 11.3AAGEE74 pKa = 4.04PTIDD78 pKa = 3.86PRR80 pKa = 11.84DD81 pKa = 3.72MVNPILWRR89 pKa = 11.84HH90 pKa = 4.22YY91 pKa = 10.22HH92 pKa = 5.89GEE94 pKa = 4.01AMLTDD99 pKa = 4.15PLARR103 pKa = 11.84TFPEE107 pKa = 4.78PYY109 pKa = 9.91GSEE112 pKa = 4.06INPGTSEE119 pKa = 4.77IIGTSIDD126 pKa = 3.63RR127 pKa = 11.84QDD129 pKa = 3.71YY130 pKa = 9.1TSGAGDD136 pKa = 3.43INQIYY141 pKa = 9.12PMCLMDD147 pKa = 4.41PSFRR151 pKa = 11.84KK152 pKa = 10.28AGVQSGFSTFVKK164 pKa = 10.0PYY166 pKa = 9.64VYY168 pKa = 10.89KK169 pKa = 10.93LATNAQLLPMRR180 pKa = 11.84GFTTPSSGAAYY191 pKa = 7.84TWNTNKK197 pKa = 9.69IEE199 pKa = 3.99PTIGGTQEE207 pKa = 4.17SYY209 pKa = 11.2LVDD212 pKa = 3.66PVLSEE217 pKa = 5.0DD218 pKa = 3.46NSSYY222 pKa = 11.31VPDD225 pKa = 4.3DD226 pKa = 3.61LKK228 pKa = 11.64GNVIFTNKK236 pKa = 9.65LDD238 pKa = 3.74RR239 pKa = 11.84LGWMDD244 pKa = 3.59TVTRR248 pKa = 11.84TLLTGSKK255 pKa = 9.2PLAVSPIPGPASSGTAIEE273 pKa = 4.78SGTGGNQTGGNFALQTYY290 pKa = 9.19LPNIPMLYY298 pKa = 10.87VMMPPAYY305 pKa = 9.43KK306 pKa = 10.24SLFYY310 pKa = 10.55FRR312 pKa = 11.84LIIKK316 pKa = 9.97HH317 pKa = 4.94YY318 pKa = 10.59FEE320 pKa = 5.4FGGFRR325 pKa = 11.84SCLNVQSYY333 pKa = 10.76GSASVYY339 pKa = 9.72VAQPLNPVTSSNVSEE354 pKa = 4.22IASMVSGIADD364 pKa = 4.46GGPDD368 pKa = 3.38SLEE371 pKa = 4.04VEE373 pKa = 4.12NGEE376 pKa = 4.33IVSSADD382 pKa = 3.1GVLL385 pKa = 3.4

Molecular weight: 41.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1R815|A0A0K1R815_9VIRU Rep protein OS=Rat associated porprismacovirus 1 OX=2170126 PE=4 SV=1
MM1 pKa = 7.59RR2 pKa = 11.84HH3 pKa = 5.85PVSMTKK9 pKa = 10.21CYY11 pKa = 10.01MITAPRR17 pKa = 11.84DD18 pKa = 3.3EE19 pKa = 4.44EE20 pKa = 4.46MYY22 pKa = 11.12ALRR25 pKa = 11.84IWRR28 pKa = 11.84YY29 pKa = 8.42INSHH33 pKa = 6.59DD34 pKa = 3.52VHH36 pKa = 7.73KK37 pKa = 10.43WIIASEE43 pKa = 3.96IGRR46 pKa = 11.84NGYY49 pKa = 8.25KK50 pKa = 9.71HH51 pKa = 4.23WQIRR55 pKa = 11.84IKK57 pKa = 10.15TSDD60 pKa = 3.39PDD62 pKa = 3.55FFSFEE67 pKa = 4.1EE68 pKa = 4.38RR69 pKa = 11.84EE70 pKa = 4.19EE71 pKa = 4.64PYY73 pKa = 9.79LTANWKK79 pKa = 9.56IEE81 pKa = 3.79YY82 pKa = 9.5RR83 pKa = 11.84KK84 pKa = 10.52VKK86 pKa = 10.31IGTGWANINIPRR98 pKa = 11.84SHH100 pKa = 8.37VEE102 pKa = 3.87EE103 pKa = 5.63CSDD106 pKa = 3.31DD107 pKa = 3.13WDD109 pKa = 4.27YY110 pKa = 10.25EE111 pKa = 4.59TKK113 pKa = 10.24EE114 pKa = 3.96GRR116 pKa = 11.84YY117 pKa = 8.71LASWDD122 pKa = 3.68TPEE125 pKa = 3.88VRR127 pKa = 11.84KK128 pKa = 10.0LRR130 pKa = 11.84FGQPRR135 pKa = 11.84WHH137 pKa = 6.37QEE139 pKa = 3.58AIINRR144 pKa = 11.84LEE146 pKa = 4.04STNDD150 pKa = 3.16RR151 pKa = 11.84EE152 pKa = 4.44VMVWYY157 pKa = 10.01DD158 pKa = 3.28PTGNSGKK165 pKa = 9.87SWLVGHH171 pKa = 7.46LYY173 pKa = 8.36EE174 pKa = 5.03TGQAYY179 pKa = 9.22YY180 pKa = 10.43LPPTMTSVQSMLQMMASLAIQDD202 pKa = 4.47RR203 pKa = 11.84EE204 pKa = 4.22DD205 pKa = 3.28GRR207 pKa = 11.84PPRR210 pKa = 11.84RR211 pKa = 11.84YY212 pKa = 9.8VVIDD216 pKa = 3.92IPRR219 pKa = 11.84TWKK222 pKa = 9.1WSKK225 pKa = 11.05DD226 pKa = 3.39LYY228 pKa = 10.91CAIEE232 pKa = 4.27TIKK235 pKa = 10.98DD236 pKa = 3.56GLIVDD241 pKa = 4.29PRR243 pKa = 11.84YY244 pKa = 9.57SARR247 pKa = 11.84PINIRR252 pKa = 11.84GVKK255 pKa = 10.05VLVLSNDD262 pKa = 3.35RR263 pKa = 11.84PSLDD267 pKa = 3.47KK268 pKa = 11.28LSVDD272 pKa = 2.63RR273 pKa = 11.84WVVEE277 pKa = 4.09NTPMLL282 pKa = 4.21

Molecular weight: 33.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

667

282

385

333.5

37.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.397 ± 0.77

0.75 ± 0.212

5.547 ± 0.803

5.247 ± 1.244

2.699 ± 0.863

7.346 ± 1.845

1.799 ± 0.461

6.597 ± 0.812

4.198 ± 0.756

7.496 ± 0.99

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.148 ± 0.268

4.348 ± 0.301

7.046 ± 0.686

2.849 ± 0.247

5.697 ± 2.136

8.396 ± 1.118

6.897 ± 1.063

6.747 ± 0.245

2.399 ± 1.491

5.397 ± 0.053
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski