Zancudomyces culisetae (Gut fungus) (Smittium culisetae)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Zoopagomycota; Kickxellomycotina; Harpellomycetes; Harpellales; Legeriomycetaceae; Zancudomyces

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7387 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1R1PSP6|A0A1R1PSP6_ZANCU Transcription initiation factor IIF subunit alpha OS=Zancudomyces culisetae OX=1213189 GN=AX774_g2511 PE=3 SV=1
MM1 pKa = 7.82LNMSDD6 pKa = 3.44SKK8 pKa = 10.4KK9 pKa = 8.41TEE11 pKa = 3.94GVISQTEE18 pKa = 3.75AKK20 pKa = 10.0LRR22 pKa = 11.84CQEE25 pKa = 3.99SSVLYY30 pKa = 9.26LTDD33 pKa = 3.28QTFSPEE39 pKa = 4.07NQLSDD44 pKa = 4.69QPDD47 pKa = 3.42QMPTHH52 pKa = 6.99PNSVVDD58 pKa = 5.47QPDD61 pKa = 3.36QEE63 pKa = 4.78PNQSDD68 pKa = 3.81PVATQPIQVPNRR80 pKa = 11.84HH81 pKa = 5.91CQMSYY86 pKa = 10.94PLDD89 pKa = 3.7TLRR92 pKa = 11.84SLPARR97 pKa = 11.84MLPEE101 pKa = 4.82PDD103 pKa = 3.11SWDD106 pKa = 3.84LEE108 pKa = 4.38HH109 pKa = 7.51SDD111 pKa = 3.51SCTEE115 pKa = 4.09DD116 pKa = 2.87EE117 pKa = 4.87CNLMEE122 pKa = 4.33YY123 pKa = 10.62SPIIVIDD130 pKa = 4.88DD131 pKa = 3.96EE132 pKa = 6.31SEE134 pKa = 3.89MCPDD138 pKa = 3.74DD139 pKa = 5.38VIYY142 pKa = 10.97LL143 pKa = 3.84

Molecular weight: 16.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1R1PKT5|A0A1R1PKT5_ZANCU Putative MFS-type transporter YusP OS=Zancudomyces culisetae OX=1213189 GN=AX774_g4965 PE=4 SV=1
TT1 pKa = 6.72TPISVARR8 pKa = 11.84MARR11 pKa = 11.84NLPLLSTTARR21 pKa = 11.84TSVSKK26 pKa = 9.83TRR28 pKa = 11.84TRR30 pKa = 11.84SHH32 pKa = 4.93KK33 pKa = 9.63RR34 pKa = 11.84ARR36 pKa = 11.84IRR38 pKa = 11.84SRR40 pKa = 11.84YY41 pKa = 7.61HH42 pKa = 7.04AKK44 pKa = 9.5TKK46 pKa = 8.58TPKK49 pKa = 10.11RR50 pKa = 11.84IQAATPTTPISVARR64 pKa = 11.84MARR67 pKa = 11.84NLPLLSTTARR77 pKa = 11.84TSVSKK82 pKa = 9.83TRR84 pKa = 11.84TRR86 pKa = 11.84SHH88 pKa = 4.93KK89 pKa = 9.63RR90 pKa = 11.84ARR92 pKa = 11.84IRR94 pKa = 11.84SRR96 pKa = 11.84YY97 pKa = 7.61HH98 pKa = 7.04AKK100 pKa = 9.5TKK102 pKa = 8.58TPKK105 pKa = 10.11RR106 pKa = 11.84IQAATPTTPISVARR120 pKa = 11.84MARR123 pKa = 11.84NLPLLSTTAKK133 pKa = 9.55TLVSKK138 pKa = 9.66TRR140 pKa = 11.84TRR142 pKa = 11.84SHH144 pKa = 4.93KK145 pKa = 9.63RR146 pKa = 11.84ARR148 pKa = 11.84IRR150 pKa = 11.84SRR152 pKa = 11.84YY153 pKa = 7.49HH154 pKa = 6.28AKK156 pKa = 9.1TRR158 pKa = 11.84TPKK161 pKa = 10.39RR162 pKa = 11.84IQTATPTTPISVARR176 pKa = 11.84MVRR179 pKa = 11.84EE180 pKa = 4.18LPVSTKK186 pKa = 9.33TVSAVLTRR194 pKa = 11.84PKK196 pKa = 10.51FFIPSTRR203 pKa = 11.84PVSSASRR210 pKa = 11.84IGVPVLRR217 pKa = 11.84GVISLLAAIYY227 pKa = 10.45GRR229 pKa = 11.84VNSMLSLTWLGSRR242 pKa = 11.84IHH244 pKa = 6.03LQQ246 pKa = 3.1

Molecular weight: 27.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7387

0

7387

2891333

8

6138

391.4

43.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.804 ± 0.028

1.326 ± 0.012

5.555 ± 0.02

6.803 ± 0.036

3.769 ± 0.02

6.213 ± 0.031

1.972 ± 0.011

6.013 ± 0.021

7.163 ± 0.033

8.344 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.175 ± 0.011

6.317 ± 0.032

4.189 ± 0.029

3.848 ± 0.026

4.618 ± 0.023

9.419 ± 0.047

6.17 ± 0.039

6.037 ± 0.023

0.806 ± 0.007

3.448 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski