Amino acid dipepetide frequency for Kluyvera phage Kvp1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.467AlaAla: 9.467 ± 1.204
1.052AlaCys: 1.052 ± 0.277
5.745AlaAsp: 5.745 ± 0.694
6.068AlaGlu: 6.068 ± 0.687
3.398AlaPhe: 3.398 ± 0.437
7.849AlaGly: 7.849 ± 0.918
1.295AlaHis: 1.295 ± 0.31
4.774AlaIle: 4.774 ± 0.567
7.363AlaLys: 7.363 ± 0.783
7.687AlaLeu: 7.687 ± 0.907
3.075AlaMet: 3.075 ± 0.546
5.097AlaAsn: 5.097 ± 0.622
2.751AlaPro: 2.751 ± 0.524
3.965AlaGln: 3.965 ± 0.416
5.178AlaArg: 5.178 ± 0.538
3.965AlaSer: 3.965 ± 0.718
2.913AlaThr: 2.913 ± 0.441
5.664AlaVal: 5.664 ± 0.672
1.537AlaTrp: 1.537 ± 0.335
2.832AlaTyr: 2.832 ± 0.543
0.0AlaXaa: 0.0 ± 0.0
Cys
0.971CysAla: 0.971 ± 0.298
0.081CysCys: 0.081 ± 0.079
0.647CysAsp: 0.647 ± 0.328
0.566CysGlu: 0.566 ± 0.21
0.566CysPhe: 0.566 ± 0.226
0.89CysGly: 0.89 ± 0.305
0.243CysHis: 0.243 ± 0.157
0.405CysIle: 0.405 ± 0.178
0.324CysLys: 0.324 ± 0.159
1.052CysLeu: 1.052 ± 0.335
0.081CysMet: 0.081 ± 0.084
0.566CysAsn: 0.566 ± 0.229
0.405CysPro: 0.405 ± 0.165
0.485CysGln: 0.485 ± 0.2
0.485CysArg: 0.485 ± 0.268
0.728CysSer: 0.728 ± 0.266
0.243CysThr: 0.243 ± 0.149
0.485CysVal: 0.485 ± 0.217
0.324CysTrp: 0.324 ± 0.155
0.405CysTyr: 0.405 ± 0.231
0.0CysXaa: 0.0 ± 0.0
Asp
4.936AspAla: 4.936 ± 0.573
0.728AspCys: 0.728 ± 0.299
4.207AspAsp: 4.207 ± 0.72
3.965AspGlu: 3.965 ± 0.566
2.67AspPhe: 2.67 ± 0.483
7.444AspGly: 7.444 ± 0.936
0.89AspHis: 0.89 ± 0.259
2.994AspIle: 2.994 ± 0.422
4.45AspLys: 4.45 ± 0.828
3.156AspLeu: 3.156 ± 0.435
3.317AspMet: 3.317 ± 0.609
2.266AspAsn: 2.266 ± 0.361
3.479AspPro: 3.479 ± 0.603
2.023AspGln: 2.023 ± 0.336
3.237AspArg: 3.237 ± 0.637
3.398AspSer: 3.398 ± 0.461
3.237AspThr: 3.237 ± 0.451
4.936AspVal: 4.936 ± 0.509
1.052AspTrp: 1.052 ± 0.425
1.295AspTyr: 1.295 ± 0.269
0.0AspXaa: 0.0 ± 0.0
Glu
7.12GluAla: 7.12 ± 0.754
0.728GluCys: 0.728 ± 0.253
4.45GluAsp: 4.45 ± 0.512
5.34GluGlu: 5.34 ± 0.93
2.913GluPhe: 2.913 ± 0.471
4.855GluGly: 4.855 ± 0.618
1.456GluHis: 1.456 ± 0.322
2.508GluIle: 2.508 ± 0.349
3.641GluLys: 3.641 ± 0.594
7.039GluLeu: 7.039 ± 0.694
2.427GluMet: 2.427 ± 0.438
3.722GluAsn: 3.722 ± 0.552
1.942GluPro: 1.942 ± 0.469
3.722GluGln: 3.722 ± 0.519
4.774GluArg: 4.774 ± 0.676
3.56GluSer: 3.56 ± 0.667
3.803GluThr: 3.803 ± 0.497
4.531GluVal: 4.531 ± 0.688
1.133GluTrp: 1.133 ± 0.276
3.237GluTyr: 3.237 ± 0.42
0.0GluXaa: 0.0 ± 0.0
Phe
2.751PheAla: 2.751 ± 0.678
0.324PheCys: 0.324 ± 0.198
2.023PheAsp: 2.023 ± 0.386
2.185PheGlu: 2.185 ± 0.361
0.89PhePhe: 0.89 ± 0.242
2.994PheGly: 2.994 ± 0.513
0.647PheHis: 0.647 ± 0.246
1.699PheIle: 1.699 ± 0.394
2.508PheLys: 2.508 ± 0.409
2.994PheLeu: 2.994 ± 0.455
1.295PheMet: 1.295 ± 0.288
1.861PheAsn: 1.861 ± 0.465
1.861PhePro: 1.861 ± 0.425
1.456PheGln: 1.456 ± 0.335
1.78PheArg: 1.78 ± 0.32
1.861PheSer: 1.861 ± 0.496
2.913PheThr: 2.913 ± 0.434
1.861PheVal: 1.861 ± 0.438
0.324PheTrp: 0.324 ± 0.145
1.214PheTyr: 1.214 ± 0.263
0.0PheXaa: 0.0 ± 0.0
Gly
6.797GlyAla: 6.797 ± 0.811
0.89GlyCys: 0.89 ± 0.359
6.958GlyAsp: 6.958 ± 0.803
5.907GlyGlu: 5.907 ± 0.622
2.67GlyPhe: 2.67 ± 0.351
6.23GlyGly: 6.23 ± 0.768
1.214GlyHis: 1.214 ± 0.354
4.369GlyIle: 4.369 ± 0.53
6.797GlyLys: 6.797 ± 0.989
7.201GlyLeu: 7.201 ± 0.978
2.104GlyMet: 2.104 ± 0.38
2.913GlyAsn: 2.913 ± 0.444
0.081GlyPro: 0.081 ± 0.072
3.237GlyGln: 3.237 ± 0.475
4.693GlyArg: 4.693 ± 0.675
5.259GlySer: 5.259 ± 0.652
3.965GlyThr: 3.965 ± 0.449
4.612GlyVal: 4.612 ± 0.674
1.942GlyTrp: 1.942 ± 0.505
2.751GlyTyr: 2.751 ± 0.483
0.0GlyXaa: 0.0 ± 0.0
His
1.537HisAla: 1.537 ± 0.404
0.324HisCys: 0.324 ± 0.16
1.133HisAsp: 1.133 ± 0.291
1.052HisGlu: 1.052 ± 0.307
0.89HisPhe: 0.89 ± 0.288
1.78HisGly: 1.78 ± 0.253
0.485HisHis: 0.485 ± 0.207
1.052HisIle: 1.052 ± 0.277
1.214HisLys: 1.214 ± 0.327
1.699HisLeu: 1.699 ± 0.343
0.566HisMet: 0.566 ± 0.191
0.647HisAsn: 0.647 ± 0.251
0.243HisPro: 0.243 ± 0.147
0.324HisGln: 0.324 ± 0.185
1.052HisArg: 1.052 ± 0.252
1.133HisSer: 1.133 ± 0.241
1.295HisThr: 1.295 ± 0.314
1.537HisVal: 1.537 ± 0.271
0.566HisTrp: 0.566 ± 0.227
0.647HisTyr: 0.647 ± 0.196
0.0HisXaa: 0.0 ± 0.0
Ile
3.965IleAla: 3.965 ± 0.449
0.405IleCys: 0.405 ± 0.214
3.722IleAsp: 3.722 ± 0.411
3.722IleGlu: 3.722 ± 0.487
0.89IlePhe: 0.89 ± 0.236
3.479IleGly: 3.479 ± 0.542
0.728IleHis: 0.728 ± 0.284
2.508IleIle: 2.508 ± 0.551
3.398IleLys: 3.398 ± 0.554
3.317IleLeu: 3.317 ± 0.496
0.728IleMet: 0.728 ± 0.245
2.023IleAsn: 2.023 ± 0.46
2.427IlePro: 2.427 ± 0.488
2.104IleGln: 2.104 ± 0.441
3.722IleArg: 3.722 ± 0.472
2.589IleSer: 2.589 ± 0.547
2.266IleThr: 2.266 ± 0.345
3.156IleVal: 3.156 ± 0.432
0.809IleTrp: 0.809 ± 0.239
1.699IleTyr: 1.699 ± 0.333
0.0IleXaa: 0.0 ± 0.0
Lys
8.01LysAla: 8.01 ± 0.877
0.809LysCys: 0.809 ± 0.267
4.774LysAsp: 4.774 ± 0.528
5.826LysGlu: 5.826 ± 0.72
1.78LysPhe: 1.78 ± 0.377
5.097LysGly: 5.097 ± 0.562
1.78LysHis: 1.78 ± 0.441
1.861LysIle: 1.861 ± 0.357
5.097LysLys: 5.097 ± 0.793
5.907LysLeu: 5.907 ± 0.728
2.185LysMet: 2.185 ± 0.321
2.427LysAsn: 2.427 ± 0.361
2.67LysPro: 2.67 ± 0.688
2.508LysGln: 2.508 ± 0.557
3.317LysArg: 3.317 ± 0.545
3.803LysSer: 3.803 ± 0.66
3.56LysThr: 3.56 ± 0.477
5.907LysVal: 5.907 ± 0.59
0.647LysTrp: 0.647 ± 0.183
2.832LysTyr: 2.832 ± 0.463
0.0LysXaa: 0.0 ± 0.0
Leu
8.658LeuAla: 8.658 ± 1.001
0.647LeuCys: 0.647 ± 0.263
4.612LeuAsp: 4.612 ± 0.425
6.473LeuGlu: 6.473 ± 0.999
2.427LeuPhe: 2.427 ± 0.394
4.45LeuGly: 4.45 ± 0.538
1.214LeuHis: 1.214 ± 0.268
4.046LeuIle: 4.046 ± 0.539
7.039LeuLys: 7.039 ± 0.748
5.017LeuLeu: 5.017 ± 0.793
2.346LeuMet: 2.346 ± 0.466
3.803LeuAsn: 3.803 ± 0.688
2.67LeuPro: 2.67 ± 0.479
3.398LeuGln: 3.398 ± 0.564
6.149LeuArg: 6.149 ± 0.476
3.56LeuSer: 3.56 ± 0.47
4.936LeuThr: 4.936 ± 0.707
4.45LeuVal: 4.45 ± 0.552
1.133LeuTrp: 1.133 ± 0.352
2.266LeuTyr: 2.266 ± 0.402
0.0LeuXaa: 0.0 ± 0.0
Met
3.075MetAla: 3.075 ± 0.588
0.162MetCys: 0.162 ± 0.113
1.618MetAsp: 1.618 ± 0.453
2.023MetGlu: 2.023 ± 0.368
0.971MetPhe: 0.971 ± 0.286
2.751MetGly: 2.751 ± 0.6
0.324MetHis: 0.324 ± 0.183
0.971MetIle: 0.971 ± 0.268
1.537MetLys: 1.537 ± 0.398
3.237MetLeu: 3.237 ± 0.504
0.647MetMet: 0.647 ± 0.221
2.023MetAsn: 2.023 ± 0.427
1.214MetPro: 1.214 ± 0.305
1.456MetGln: 1.456 ± 0.344
1.052MetArg: 1.052 ± 0.298
2.266MetSer: 2.266 ± 0.246
1.456MetThr: 1.456 ± 0.386
2.185MetVal: 2.185 ± 0.474
0.081MetTrp: 0.081 ± 0.08
0.728MetTyr: 0.728 ± 0.236
0.0MetXaa: 0.0 ± 0.0
Asn
3.641AsnAla: 3.641 ± 0.447
0.405AsnCys: 0.405 ± 0.153
2.589AsnAsp: 2.589 ± 0.455
3.317AsnGlu: 3.317 ± 0.529
1.618AsnPhe: 1.618 ± 0.309
5.178AsnGly: 5.178 ± 0.886
1.295AsnHis: 1.295 ± 0.323
2.508AsnIle: 2.508 ± 0.462
2.994AsnLys: 2.994 ± 0.47
3.56AsnLeu: 3.56 ± 0.503
1.214AsnMet: 1.214 ± 0.379
1.618AsnAsn: 1.618 ± 0.471
2.589AsnPro: 2.589 ± 0.356
1.456AsnGln: 1.456 ± 0.329
2.266AsnArg: 2.266 ± 0.619
2.67AsnSer: 2.67 ± 0.679
2.266AsnThr: 2.266 ± 0.489
2.67AsnVal: 2.67 ± 0.395
0.647AsnTrp: 0.647 ± 0.208
1.942AsnTyr: 1.942 ± 0.349
0.0AsnXaa: 0.0 ± 0.0
Pro
2.427ProAla: 2.427 ± 0.405
0.405ProCys: 0.405 ± 0.203
2.508ProAsp: 2.508 ± 0.364
3.641ProGlu: 3.641 ± 0.648
1.376ProPhe: 1.376 ± 0.337
1.456ProGly: 1.456 ± 0.286
0.647ProHis: 0.647 ± 0.254
1.537ProIle: 1.537 ± 0.297
2.832ProLys: 2.832 ± 0.533
2.185ProLeu: 2.185 ± 0.356
0.89ProMet: 0.89 ± 0.381
2.266ProAsn: 2.266 ± 0.405
0.728ProPro: 0.728 ± 0.25
0.971ProGln: 0.971 ± 0.248
1.537ProArg: 1.537 ± 0.407
2.346ProSer: 2.346 ± 0.43
2.023ProThr: 2.023 ± 0.38
2.994ProVal: 2.994 ± 0.565
0.566ProTrp: 0.566 ± 0.186
1.295ProTyr: 1.295 ± 0.328
0.0ProXaa: 0.0 ± 0.0
Gln
4.774GlnAla: 4.774 ± 0.515
0.162GlnCys: 0.162 ± 0.125
1.861GlnAsp: 1.861 ± 0.369
2.427GlnGlu: 2.427 ± 0.448
2.185GlnPhe: 2.185 ± 0.297
2.994GlnGly: 2.994 ± 0.472
0.809GlnHis: 0.809 ± 0.384
1.942GlnIle: 1.942 ± 0.312
1.861GlnLys: 1.861 ± 0.446
3.317GlnLeu: 3.317 ± 0.586
0.971GlnMet: 0.971 ± 0.255
0.971GlnAsn: 0.971 ± 0.254
1.376GlnPro: 1.376 ± 0.412
1.861GlnGln: 1.861 ± 0.351
1.618GlnArg: 1.618 ± 0.397
2.185GlnSer: 2.185 ± 0.405
1.942GlnThr: 1.942 ± 0.416
2.266GlnVal: 2.266 ± 0.437
0.89GlnTrp: 0.89 ± 0.255
1.78GlnTyr: 1.78 ± 0.428
0.0GlnXaa: 0.0 ± 0.0
Arg
4.369ArgAla: 4.369 ± 0.539
0.728ArgCys: 0.728 ± 0.267
3.156ArgAsp: 3.156 ± 0.468
4.693ArgGlu: 4.693 ± 0.667
2.104ArgPhe: 2.104 ± 0.5
4.207ArgGly: 4.207 ± 0.514
1.052ArgHis: 1.052 ± 0.298
2.508ArgIle: 2.508 ± 0.397
4.693ArgLys: 4.693 ± 0.739
4.855ArgLeu: 4.855 ± 0.639
1.376ArgMet: 1.376 ± 0.292
3.479ArgAsn: 3.479 ± 0.651
1.942ArgPro: 1.942 ± 0.297
1.699ArgGln: 1.699 ± 0.347
2.67ArgArg: 2.67 ± 0.469
3.803ArgSer: 3.803 ± 0.504
2.832ArgThr: 2.832 ± 0.442
3.641ArgVal: 3.641 ± 0.619
0.485ArgTrp: 0.485 ± 0.223
1.618ArgTyr: 1.618 ± 0.294
0.0ArgXaa: 0.0 ± 0.0
Ser
5.259SerAla: 5.259 ± 0.872
0.485SerCys: 0.485 ± 0.225
4.046SerAsp: 4.046 ± 0.583
3.075SerGlu: 3.075 ± 0.537
2.346SerPhe: 2.346 ± 0.33
5.178SerGly: 5.178 ± 0.661
1.537SerHis: 1.537 ± 0.394
3.237SerIle: 3.237 ± 0.464
3.884SerLys: 3.884 ± 0.553
3.722SerLeu: 3.722 ± 0.432
1.456SerMet: 1.456 ± 0.365
2.913SerAsn: 2.913 ± 0.47
1.861SerPro: 1.861 ± 0.341
1.537SerGln: 1.537 ± 0.416
3.317SerArg: 3.317 ± 0.463
2.023SerSer: 2.023 ± 0.424
3.075SerThr: 3.075 ± 0.456
3.722SerVal: 3.722 ± 0.51
0.324SerTrp: 0.324 ± 0.124
2.427SerTyr: 2.427 ± 0.661
0.0SerXaa: 0.0 ± 0.0
Thr
3.722ThrAla: 3.722 ± 0.614
0.566ThrCys: 0.566 ± 0.25
2.751ThrAsp: 2.751 ± 0.407
4.45ThrGlu: 4.45 ± 0.456
2.023ThrPhe: 2.023 ± 0.43
5.259ThrGly: 5.259 ± 0.673
1.052ThrHis: 1.052 ± 0.282
3.156ThrIle: 3.156 ± 0.491
3.56ThrLys: 3.56 ± 0.511
4.612ThrLeu: 4.612 ± 0.692
1.295ThrMet: 1.295 ± 0.283
1.618ThrAsn: 1.618 ± 0.398
2.346ThrPro: 2.346 ± 0.324
1.456ThrGln: 1.456 ± 0.28
2.508ThrArg: 2.508 ± 0.43
3.722ThrSer: 3.722 ± 0.639
2.832ThrThr: 2.832 ± 0.521
3.965ThrVal: 3.965 ± 0.589
0.89ThrTrp: 0.89 ± 0.334
1.295ThrTyr: 1.295 ± 0.312
0.0ThrXaa: 0.0 ± 0.0
Val
6.23ValAla: 6.23 ± 0.603
0.405ValCys: 0.405 ± 0.191
3.884ValAsp: 3.884 ± 0.49
4.936ValGlu: 4.936 ± 0.731
2.751ValPhe: 2.751 ± 0.676
4.369ValGly: 4.369 ± 0.622
1.376ValHis: 1.376 ± 0.325
3.641ValIle: 3.641 ± 0.537
4.45ValLys: 4.45 ± 0.581
4.936ValLeu: 4.936 ± 0.692
2.023ValMet: 2.023 ± 0.422
3.398ValAsn: 3.398 ± 0.731
2.427ValPro: 2.427 ± 0.437
2.427ValGln: 2.427 ± 0.61
4.127ValArg: 4.127 ± 0.606
3.722ValSer: 3.722 ± 0.567
4.45ValThr: 4.45 ± 0.529
4.046ValVal: 4.046 ± 0.571
0.89ValTrp: 0.89 ± 0.406
1.942ValTyr: 1.942 ± 0.469
0.0ValXaa: 0.0 ± 0.0
Trp
0.485TrpAla: 0.485 ± 0.251
0.243TrpCys: 0.243 ± 0.16
0.405TrpAsp: 0.405 ± 0.179
1.052TrpGlu: 1.052 ± 0.225
0.405TrpPhe: 0.405 ± 0.183
0.971TrpGly: 0.971 ± 0.322
0.485TrpHis: 0.485 ± 0.25
0.485TrpIle: 0.485 ± 0.213
1.537TrpLys: 1.537 ± 0.32
1.456TrpLeu: 1.456 ± 0.394
0.405TrpMet: 0.405 ± 0.152
1.052TrpAsn: 1.052 ± 0.31
0.405TrpPro: 0.405 ± 0.178
0.809TrpGln: 0.809 ± 0.29
0.89TrpArg: 0.89 ± 0.25
0.971TrpSer: 0.971 ± 0.336
1.052TrpThr: 1.052 ± 0.32
1.295TrpVal: 1.295 ± 0.334
0.405TrpTrp: 0.405 ± 0.211
0.405TrpTyr: 0.405 ± 0.184
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.479TyrAla: 3.479 ± 0.452
0.405TyrCys: 0.405 ± 0.181
2.589TyrAsp: 2.589 ± 0.434
2.185TyrGlu: 2.185 ± 0.386
0.566TyrPhe: 0.566 ± 0.23
3.156TyrGly: 3.156 ± 0.46
0.647TyrHis: 0.647 ± 0.195
1.456TyrIle: 1.456 ± 0.4
1.618TyrLys: 1.618 ± 0.438
2.346TyrLeu: 2.346 ± 0.437
1.295TyrMet: 1.295 ± 0.307
1.78TyrAsn: 1.78 ± 0.427
1.295TyrPro: 1.295 ± 0.323
1.295TyrGln: 1.295 ± 0.412
1.537TyrArg: 1.537 ± 0.305
1.861TyrSer: 1.861 ± 0.323
2.023TyrThr: 2.023 ± 0.475
2.508TyrVal: 2.508 ± 0.408
0.566TyrTrp: 0.566 ± 0.236
1.133TyrTyr: 1.133 ± 0.288
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 47 proteins (12360 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski