Clostridium sp. AM58-1XD

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; unclassified Clostridium

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3528 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A395YN96|A0A395YN96_9CLOT LytR_cpsA_psr domain-containing protein OS=Clostridium sp. AM58-1XD OX=2292307 GN=DXA13_06840 PE=3 SV=1
MM1 pKa = 7.66EE2 pKa = 4.4KK3 pKa = 10.44QEE5 pKa = 4.09IKK7 pKa = 10.7EE8 pKa = 4.51GFLSWLDD15 pKa = 3.43SSLEE19 pKa = 4.04QEE21 pKa = 5.25FPTEE25 pKa = 3.59MAAVNFNLYY34 pKa = 10.68DD35 pKa = 4.74DD36 pKa = 6.1GDD38 pKa = 4.34DD39 pKa = 3.39QWSSEE44 pKa = 4.1LVGTSSFDD52 pKa = 4.01EE53 pKa = 4.83NDD55 pKa = 4.19DD56 pKa = 4.05DD57 pKa = 4.21WACDD61 pKa = 3.32EE62 pKa = 4.61VYY64 pKa = 11.08SNRR67 pKa = 11.84DD68 pKa = 2.95HH69 pKa = 7.62LYY71 pKa = 10.51VFDD74 pKa = 5.88CAMEE78 pKa = 4.14WEE80 pKa = 4.66DD81 pKa = 4.96VLNLICDD88 pKa = 3.76SVKK91 pKa = 10.49EE92 pKa = 3.88YY93 pKa = 11.32LEE95 pKa = 4.34NGRR98 pKa = 11.84HH99 pKa = 6.12ADD101 pKa = 3.64TLKK104 pKa = 10.37GLQGVGVGFVDD115 pKa = 3.82GDD117 pKa = 3.51ITVVCKK123 pKa = 10.6RR124 pKa = 11.84GCC126 pKa = 3.38

Molecular weight: 14.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A395YF90|A0A395YF90_9CLOT Dihydrodipicolinate synthase family protein OS=Clostridium sp. AM58-1XD OX=2292307 GN=DXA13_17715 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.71MTFQPKK8 pKa = 7.43TRR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.26VHH16 pKa = 5.59GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSAGGRR28 pKa = 11.84KK29 pKa = 8.81VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.33GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 4.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3528

0

3528

1073545

20

3093

304.3

33.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.919 ± 0.038

1.561 ± 0.02

5.422 ± 0.032

7.598 ± 0.055

4.093 ± 0.029

7.734 ± 0.043

1.67 ± 0.018

7.366 ± 0.04

6.28 ± 0.037

8.835 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.38 ± 0.023

3.975 ± 0.027

3.467 ± 0.021

3.046 ± 0.021

4.713 ± 0.04

5.94 ± 0.036

5.244 ± 0.034

6.828 ± 0.036

0.972 ± 0.015

3.956 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski