Amino acid dipepetide frequency for Gordonia phage Horus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.435AlaAla: 13.435 ± 1.066
0.964AlaCys: 0.964 ± 0.257
7.766AlaAsp: 7.766 ± 0.846
6.972AlaGlu: 6.972 ± 0.814
2.608AlaPhe: 2.608 ± 0.656
9.126AlaGly: 9.126 ± 0.929
1.984AlaHis: 1.984 ± 0.406
6.292AlaIle: 6.292 ± 0.696
3.741AlaLys: 3.741 ± 0.471
9.183AlaLeu: 9.183 ± 0.779
3.515AlaMet: 3.515 ± 0.495
2.664AlaAsn: 2.664 ± 0.333
5.158AlaPro: 5.158 ± 0.749
5.045AlaGln: 5.045 ± 0.579
7.426AlaArg: 7.426 ± 0.736
6.406AlaSer: 6.406 ± 0.613
6.009AlaThr: 6.009 ± 0.619
9.523AlaVal: 9.523 ± 1.022
2.097AlaTrp: 2.097 ± 0.374
2.494AlaTyr: 2.494 ± 0.394
0.0AlaXaa: 0.0 ± 0.0
Cys
0.907CysAla: 0.907 ± 0.274
0.283CysCys: 0.283 ± 0.128
0.907CysAsp: 0.907 ± 0.244
0.85CysGlu: 0.85 ± 0.255
0.227CysPhe: 0.227 ± 0.129
1.701CysGly: 1.701 ± 0.408
0.51CysHis: 0.51 ± 0.23
0.283CysIle: 0.283 ± 0.111
0.453CysLys: 0.453 ± 0.174
0.624CysLeu: 0.624 ± 0.182
0.057CysMet: 0.057 ± 0.065
0.453CysAsn: 0.453 ± 0.202
0.51CysPro: 0.51 ± 0.209
0.453CysGln: 0.453 ± 0.176
1.134CysArg: 1.134 ± 0.327
0.624CysSer: 0.624 ± 0.246
0.453CysThr: 0.453 ± 0.153
0.964CysVal: 0.964 ± 0.246
0.283CysTrp: 0.283 ± 0.126
0.283CysTyr: 0.283 ± 0.11
0.0CysXaa: 0.0 ± 0.0
Asp
7.539AspAla: 7.539 ± 0.732
0.624AspCys: 0.624 ± 0.207
4.081AspAsp: 4.081 ± 0.646
4.251AspGlu: 4.251 ± 0.471
1.531AspPhe: 1.531 ± 0.308
5.895AspGly: 5.895 ± 0.675
1.077AspHis: 1.077 ± 0.246
2.494AspIle: 2.494 ± 0.439
2.097AspLys: 2.097 ± 0.356
5.499AspLeu: 5.499 ± 0.656
1.247AspMet: 1.247 ± 0.231
1.757AspAsn: 1.757 ± 0.327
4.251AspPro: 4.251 ± 0.483
1.644AspGln: 1.644 ± 0.336
4.762AspArg: 4.762 ± 0.621
4.025AspSer: 4.025 ± 0.556
3.741AspThr: 3.741 ± 0.466
5.385AspVal: 5.385 ± 0.477
1.247AspTrp: 1.247 ± 0.265
1.814AspTyr: 1.814 ± 0.336
0.0AspXaa: 0.0 ± 0.0
Glu
6.916GluAla: 6.916 ± 0.719
0.51GluCys: 0.51 ± 0.176
3.174GluAsp: 3.174 ± 0.477
2.721GluGlu: 2.721 ± 0.515
2.097GluPhe: 2.097 ± 0.386
4.025GluGly: 4.025 ± 0.525
1.701GluHis: 1.701 ± 0.295
2.211GluIle: 2.211 ± 0.375
2.381GluLys: 2.381 ± 0.47
5.385GluLeu: 5.385 ± 0.603
1.19GluMet: 1.19 ± 0.227
0.85GluAsn: 0.85 ± 0.228
3.061GluPro: 3.061 ± 0.472
2.267GluGln: 2.267 ± 0.421
4.251GluArg: 4.251 ± 0.555
3.004GluSer: 3.004 ± 0.484
3.004GluThr: 3.004 ± 0.377
4.648GluVal: 4.648 ± 0.553
1.587GluTrp: 1.587 ± 0.394
1.19GluTyr: 1.19 ± 0.299
0.0GluXaa: 0.0 ± 0.0
Phe
3.004PheAla: 3.004 ± 0.421
0.397PheCys: 0.397 ± 0.2
1.757PheAsp: 1.757 ± 0.347
1.134PheGlu: 1.134 ± 0.238
0.794PhePhe: 0.794 ± 0.215
2.948PheGly: 2.948 ± 0.438
0.624PheHis: 0.624 ± 0.173
1.02PheIle: 1.02 ± 0.261
0.964PheLys: 0.964 ± 0.223
1.757PheLeu: 1.757 ± 0.337
0.964PheMet: 0.964 ± 0.303
0.907PheAsn: 0.907 ± 0.267
1.19PhePro: 1.19 ± 0.253
0.907PheGln: 0.907 ± 0.187
1.984PheArg: 1.984 ± 0.358
1.417PheSer: 1.417 ± 0.329
2.324PheThr: 2.324 ± 0.307
1.757PheVal: 1.757 ± 0.318
0.453PheTrp: 0.453 ± 0.177
0.964PheTyr: 0.964 ± 0.215
0.0PheXaa: 0.0 ± 0.0
Gly
7.879GlyAla: 7.879 ± 1.159
0.794GlyCys: 0.794 ± 0.202
5.102GlyAsp: 5.102 ± 0.669
3.911GlyGlu: 3.911 ± 0.44
3.741GlyPhe: 3.741 ± 0.565
8.106GlyGly: 8.106 ± 1.161
1.871GlyHis: 1.871 ± 0.324
4.308GlyIle: 4.308 ± 0.688
3.458GlyLys: 3.458 ± 0.457
6.406GlyLeu: 6.406 ± 0.547
2.211GlyMet: 2.211 ± 0.37
2.154GlyAsn: 2.154 ± 0.382
3.401GlyPro: 3.401 ± 0.412
2.834GlyGln: 2.834 ± 0.388
6.009GlyArg: 6.009 ± 0.458
5.045GlySer: 5.045 ± 0.596
5.215GlyThr: 5.215 ± 0.569
7.766GlyVal: 7.766 ± 0.666
2.494GlyTrp: 2.494 ± 0.318
1.757GlyTyr: 1.757 ± 0.266
0.0GlyXaa: 0.0 ± 0.0
His
2.041HisAla: 2.041 ± 0.334
0.283HisCys: 0.283 ± 0.115
1.36HisAsp: 1.36 ± 0.3
1.247HisGlu: 1.247 ± 0.286
0.624HisPhe: 0.624 ± 0.183
1.871HisGly: 1.871 ± 0.3
0.51HisHis: 0.51 ± 0.192
0.964HisIle: 0.964 ± 0.245
0.567HisLys: 0.567 ± 0.248
2.551HisLeu: 2.551 ± 0.473
0.453HisMet: 0.453 ± 0.153
0.453HisAsn: 0.453 ± 0.146
0.737HisPro: 0.737 ± 0.186
0.68HisGln: 0.68 ± 0.206
1.587HisArg: 1.587 ± 0.315
0.85HisSer: 0.85 ± 0.233
1.36HisThr: 1.36 ± 0.244
2.097HisVal: 2.097 ± 0.318
0.85HisTrp: 0.85 ± 0.27
1.02HisTyr: 1.02 ± 0.224
0.0HisXaa: 0.0 ± 0.0
Ile
5.952IleAla: 5.952 ± 0.584
0.567IleCys: 0.567 ± 0.226
4.138IleAsp: 4.138 ± 0.51
2.664IleGlu: 2.664 ± 0.467
1.077IlePhe: 1.077 ± 0.352
4.308IleGly: 4.308 ± 0.513
0.737IleHis: 0.737 ± 0.227
1.36IleIle: 1.36 ± 0.375
1.871IleLys: 1.871 ± 0.52
3.571IleLeu: 3.571 ± 0.464
0.68IleMet: 0.68 ± 0.202
1.757IleAsn: 1.757 ± 0.321
2.494IlePro: 2.494 ± 0.318
1.644IleGln: 1.644 ± 0.277
2.948IleArg: 2.948 ± 0.307
2.438IleSer: 2.438 ± 0.349
3.288IleThr: 3.288 ± 0.487
3.401IleVal: 3.401 ± 0.438
0.794IleTrp: 0.794 ± 0.222
0.85IleTyr: 0.85 ± 0.241
0.0IleXaa: 0.0 ± 0.0
Lys
4.025LysAla: 4.025 ± 0.559
0.283LysCys: 0.283 ± 0.12
2.551LysAsp: 2.551 ± 0.477
1.644LysGlu: 1.644 ± 0.317
1.247LysPhe: 1.247 ± 0.253
2.608LysGly: 2.608 ± 0.597
0.85LysHis: 0.85 ± 0.234
1.304LysIle: 1.304 ± 0.259
2.381LysLys: 2.381 ± 0.458
3.061LysLeu: 3.061 ± 0.374
0.397LysMet: 0.397 ± 0.15
1.077LysAsn: 1.077 ± 0.248
2.267LysPro: 2.267 ± 0.455
1.19LysGln: 1.19 ± 0.298
2.438LysArg: 2.438 ± 0.469
2.041LysSer: 2.041 ± 0.39
2.948LysThr: 2.948 ± 0.463
2.608LysVal: 2.608 ± 0.384
0.737LysTrp: 0.737 ± 0.175
0.907LysTyr: 0.907 ± 0.223
0.0LysXaa: 0.0 ± 0.0
Leu
10.714LeuAla: 10.714 ± 0.807
0.907LeuCys: 0.907 ± 0.252
4.422LeuAsp: 4.422 ± 0.588
4.308LeuGlu: 4.308 ± 0.593
1.984LeuPhe: 1.984 ± 0.473
6.235LeuGly: 6.235 ± 0.551
1.644LeuHis: 1.644 ± 0.367
3.515LeuIle: 3.515 ± 0.501
2.381LeuLys: 2.381 ± 0.36
5.442LeuLeu: 5.442 ± 0.753
1.531LeuMet: 1.531 ± 0.285
1.474LeuAsn: 1.474 ± 0.279
4.932LeuPro: 4.932 ± 0.554
2.608LeuGln: 2.608 ± 0.413
5.612LeuArg: 5.612 ± 0.593
5.102LeuSer: 5.102 ± 0.543
5.499LeuThr: 5.499 ± 0.594
6.122LeuVal: 6.122 ± 0.668
1.701LeuTrp: 1.701 ± 0.388
1.644LeuTyr: 1.644 ± 0.319
0.0LeuXaa: 0.0 ± 0.0
Met
2.664MetAla: 2.664 ± 0.447
0.283MetCys: 0.283 ± 0.122
1.304MetAsp: 1.304 ± 0.261
0.624MetGlu: 0.624 ± 0.196
0.397MetPhe: 0.397 ± 0.17
1.531MetGly: 1.531 ± 0.321
0.567MetHis: 0.567 ± 0.201
1.19MetIle: 1.19 ± 0.292
0.85MetLys: 0.85 ± 0.221
1.36MetLeu: 1.36 ± 0.288
0.624MetMet: 0.624 ± 0.176
0.624MetAsn: 0.624 ± 0.17
1.417MetPro: 1.417 ± 0.319
0.51MetGln: 0.51 ± 0.173
2.097MetArg: 2.097 ± 0.505
2.608MetSer: 2.608 ± 0.391
2.778MetThr: 2.778 ± 0.44
1.531MetVal: 1.531 ± 0.303
0.397MetTrp: 0.397 ± 0.164
0.34MetTyr: 0.34 ± 0.12
0.0MetXaa: 0.0 ± 0.0
Asn
3.741AsnAla: 3.741 ± 0.633
0.453AsnCys: 0.453 ± 0.181
1.701AsnAsp: 1.701 ± 0.288
1.531AsnGlu: 1.531 ± 0.338
0.567AsnPhe: 0.567 ± 0.202
3.118AsnGly: 3.118 ± 0.381
0.624AsnHis: 0.624 ± 0.155
1.304AsnIle: 1.304 ± 0.402
0.453AsnLys: 0.453 ± 0.159
2.267AsnLeu: 2.267 ± 0.346
0.34AsnMet: 0.34 ± 0.107
0.737AsnAsn: 0.737 ± 0.196
2.324AsnPro: 2.324 ± 0.297
0.85AsnGln: 0.85 ± 0.25
2.267AsnArg: 2.267 ± 0.419
1.19AsnSer: 1.19 ± 0.254
1.701AsnThr: 1.701 ± 0.294
1.474AsnVal: 1.474 ± 0.275
0.227AsnTrp: 0.227 ± 0.107
0.794AsnTyr: 0.794 ± 0.192
0.0AsnXaa: 0.0 ± 0.0
Pro
5.669ProAla: 5.669 ± 0.575
0.737ProCys: 0.737 ± 0.209
3.855ProAsp: 3.855 ± 0.572
3.628ProGlu: 3.628 ± 0.47
1.077ProPhe: 1.077 ± 0.242
4.705ProGly: 4.705 ± 0.515
0.85ProHis: 0.85 ± 0.201
2.438ProIle: 2.438 ± 0.389
2.834ProLys: 2.834 ± 0.396
3.515ProLeu: 3.515 ± 0.502
1.474ProMet: 1.474 ± 0.266
1.701ProAsn: 1.701 ± 0.354
2.834ProPro: 2.834 ± 0.571
1.134ProGln: 1.134 ± 0.292
3.061ProArg: 3.061 ± 0.398
3.628ProSer: 3.628 ± 0.525
4.025ProThr: 4.025 ± 0.428
4.478ProVal: 4.478 ± 0.548
0.964ProTrp: 0.964 ± 0.243
1.304ProTyr: 1.304 ± 0.319
0.0ProXaa: 0.0 ± 0.0
Gln
4.138GlnAla: 4.138 ± 0.598
0.68GlnCys: 0.68 ± 0.198
1.531GlnAsp: 1.531 ± 0.302
1.871GlnGlu: 1.871 ± 0.29
1.19GlnPhe: 1.19 ± 0.228
2.608GlnGly: 2.608 ± 0.443
0.624GlnHis: 0.624 ± 0.182
2.267GlnIle: 2.267 ± 0.332
0.907GlnLys: 0.907 ± 0.229
2.778GlnLeu: 2.778 ± 0.407
1.077GlnMet: 1.077 ± 0.242
1.19GlnAsn: 1.19 ± 0.276
1.644GlnPro: 1.644 ± 0.226
1.757GlnGln: 1.757 ± 0.369
2.891GlnArg: 2.891 ± 0.382
1.984GlnSer: 1.984 ± 0.291
1.927GlnThr: 1.927 ± 0.391
2.267GlnVal: 2.267 ± 0.374
1.02GlnTrp: 1.02 ± 0.251
0.794GlnTyr: 0.794 ± 0.206
0.0GlnXaa: 0.0 ± 0.0
Arg
7.256ArgAla: 7.256 ± 0.623
1.19ArgCys: 1.19 ± 0.273
4.648ArgAsp: 4.648 ± 0.552
4.478ArgGlu: 4.478 ± 0.401
1.984ArgPhe: 1.984 ± 0.381
4.762ArgGly: 4.762 ± 0.648
2.267ArgHis: 2.267 ± 0.403
3.968ArgIle: 3.968 ± 0.448
3.061ArgLys: 3.061 ± 0.496
6.122ArgLeu: 6.122 ± 0.519
1.927ArgMet: 1.927 ± 0.279
2.211ArgAsn: 2.211 ± 0.382
2.948ArgPro: 2.948 ± 0.386
2.891ArgGln: 2.891 ± 0.455
6.292ArgArg: 6.292 ± 0.913
3.515ArgSer: 3.515 ± 0.5
4.025ArgThr: 4.025 ± 0.447
4.251ArgVal: 4.251 ± 0.58
1.871ArgTrp: 1.871 ± 0.327
2.097ArgTyr: 2.097 ± 0.378
0.0ArgXaa: 0.0 ± 0.0
Ser
5.952SerAla: 5.952 ± 0.49
0.624SerCys: 0.624 ± 0.224
3.344SerAsp: 3.344 ± 0.505
3.344SerGlu: 3.344 ± 0.471
1.417SerPhe: 1.417 ± 0.259
6.462SerGly: 6.462 ± 0.861
1.36SerHis: 1.36 ± 0.327
2.438SerIle: 2.438 ± 0.354
2.041SerLys: 2.041 ± 0.326
4.308SerLeu: 4.308 ± 0.544
2.211SerMet: 2.211 ± 0.329
1.474SerAsn: 1.474 ± 0.33
2.324SerPro: 2.324 ± 0.314
1.757SerGln: 1.757 ± 0.306
4.081SerArg: 4.081 ± 0.58
4.592SerSer: 4.592 ± 0.688
4.138SerThr: 4.138 ± 0.502
4.592SerVal: 4.592 ± 0.672
1.077SerTrp: 1.077 ± 0.211
1.474SerTyr: 1.474 ± 0.317
0.0SerXaa: 0.0 ± 0.0
Thr
7.936ThrAla: 7.936 ± 0.855
0.85ThrCys: 0.85 ± 0.23
4.138ThrAsp: 4.138 ± 0.44
3.061ThrGlu: 3.061 ± 0.503
1.644ThrPhe: 1.644 ± 0.268
4.365ThrGly: 4.365 ± 0.572
1.531ThrHis: 1.531 ± 0.293
3.628ThrIle: 3.628 ± 0.481
1.701ThrLys: 1.701 ± 0.317
5.839ThrLeu: 5.839 ± 0.549
0.964ThrMet: 0.964 ± 0.258
1.814ThrAsn: 1.814 ± 0.3
4.818ThrPro: 4.818 ± 0.486
2.551ThrGln: 2.551 ± 0.376
3.741ThrArg: 3.741 ± 0.405
3.401ThrSer: 3.401 ± 0.581
4.195ThrThr: 4.195 ± 0.526
5.612ThrVal: 5.612 ± 0.575
1.247ThrTrp: 1.247 ± 0.305
1.814ThrTyr: 1.814 ± 0.328
0.0ThrXaa: 0.0 ± 0.0
Val
8.786ValAla: 8.786 ± 0.723
0.737ValCys: 0.737 ± 0.262
4.932ValAsp: 4.932 ± 0.534
5.442ValGlu: 5.442 ± 0.502
1.984ValPhe: 1.984 ± 0.312
6.122ValGly: 6.122 ± 0.664
1.644ValHis: 1.644 ± 0.282
3.741ValIle: 3.741 ± 0.46
2.494ValLys: 2.494 ± 0.506
4.648ValLeu: 4.648 ± 0.44
1.474ValMet: 1.474 ± 0.357
2.381ValAsn: 2.381 ± 0.454
5.102ValPro: 5.102 ± 0.533
3.004ValGln: 3.004 ± 0.453
5.385ValArg: 5.385 ± 0.669
4.365ValSer: 4.365 ± 0.672
4.818ValThr: 4.818 ± 0.579
6.406ValVal: 6.406 ± 0.744
2.097ValTrp: 2.097 ± 0.382
1.814ValTyr: 1.814 ± 0.406
0.0ValXaa: 0.0 ± 0.0
Trp
1.871TrpAla: 1.871 ± 0.354
0.51TrpCys: 0.51 ± 0.168
1.701TrpAsp: 1.701 ± 0.324
0.85TrpGlu: 0.85 ± 0.211
0.34TrpPhe: 0.34 ± 0.121
1.134TrpGly: 1.134 ± 0.245
0.567TrpHis: 0.567 ± 0.201
1.304TrpIle: 1.304 ± 0.222
0.85TrpLys: 0.85 ± 0.24
2.097TrpLeu: 2.097 ± 0.33
0.964TrpMet: 0.964 ± 0.208
1.474TrpAsn: 1.474 ± 0.29
1.19TrpPro: 1.19 ± 0.263
0.907TrpGln: 0.907 ± 0.18
1.984TrpArg: 1.984 ± 0.374
0.964TrpSer: 0.964 ± 0.246
1.417TrpThr: 1.417 ± 0.279
0.964TrpVal: 0.964 ± 0.222
0.51TrpTrp: 0.51 ± 0.197
0.453TrpTyr: 0.453 ± 0.137
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.984TyrAla: 1.984 ± 0.376
0.283TyrCys: 0.283 ± 0.119
2.608TyrAsp: 2.608 ± 0.36
1.644TyrGlu: 1.644 ± 0.345
0.737TyrPhe: 0.737 ± 0.192
2.834TyrGly: 2.834 ± 0.414
0.567TyrHis: 0.567 ± 0.189
0.51TyrIle: 0.51 ± 0.181
1.077TyrLys: 1.077 ± 0.242
1.36TyrLeu: 1.36 ± 0.302
0.34TyrMet: 0.34 ± 0.152
0.51TyrAsn: 0.51 ± 0.145
1.304TyrPro: 1.304 ± 0.31
0.51TyrGln: 0.51 ± 0.182
1.814TyrArg: 1.814 ± 0.371
1.814TyrSer: 1.814 ± 0.337
1.984TyrThr: 1.984 ± 0.358
1.531TyrVal: 1.531 ± 0.27
0.397TyrTrp: 0.397 ± 0.163
0.51TyrTyr: 0.51 ± 0.152
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 103 proteins (17642 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski