Erwinia phage phiEa2809

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Ackermannviridae; Nezavisimistyvirus; Erwinia virus Ea2809

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 145 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0YR36|A0A0A0YR36_9CAUD Putative regulatory protein FmdB family OS=Erwinia phage phiEa2809 OX=1564096 GN=NW77_049 PE=4 SV=1
MM1 pKa = 7.56TDD3 pKa = 3.56DD4 pKa = 4.9YY5 pKa = 11.37YY6 pKa = 11.6AQRR9 pKa = 11.84KK10 pKa = 7.26FFRR13 pKa = 11.84KK14 pKa = 9.92NMMLEE19 pKa = 4.03GLVEE23 pKa = 4.0QQILLLLMEE32 pKa = 4.8VNPHH36 pKa = 6.24FKK38 pKa = 10.78SEE40 pKa = 4.16LYY42 pKa = 9.01YY43 pKa = 9.69QLRR46 pKa = 11.84LVVHH50 pKa = 6.0SVEE53 pKa = 5.54IIDD56 pKa = 5.45DD57 pKa = 4.3DD58 pKa = 4.29FLVAHH63 pKa = 6.56VEE65 pKa = 4.21HH66 pKa = 6.42QLLNPDD72 pKa = 3.49MRR74 pKa = 11.84PIGRR78 pKa = 11.84PQFCQVEE85 pKa = 4.23LEE87 pKa = 4.33YY88 pKa = 11.36VFVDD92 pKa = 3.21LTVFNTSNHH101 pKa = 6.01GDD103 pKa = 3.57NDD105 pKa = 4.28CPDD108 pKa = 3.98EE109 pKa = 5.32DD110 pKa = 5.51LDD112 pKa = 5.74DD113 pKa = 4.45VPVDD117 pKa = 3.45NTEE120 pKa = 3.67PWEE123 pKa = 4.33TII125 pKa = 3.33

Molecular weight: 14.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0YS92|A0A0A0YS92_9CAUD VWFA domain-containing protein OS=Erwinia phage phiEa2809 OX=1564096 GN=NW77_086 PE=4 SV=1
MM1 pKa = 7.56AMSKK5 pKa = 10.52LKK7 pKa = 10.99VIGAIMAFTLATGVSASCDD26 pKa = 3.39VAFTSSQLDD35 pKa = 3.56VMTRR39 pKa = 11.84AYY41 pKa = 7.21TTGKK45 pKa = 10.44KK46 pKa = 9.86SDD48 pKa = 3.49LGYY51 pKa = 9.24TLAAISWRR59 pKa = 11.84EE60 pKa = 3.82SKK62 pKa = 10.68AGQDD66 pKa = 3.56VVRR69 pKa = 11.84MGKK72 pKa = 8.07SVKK75 pKa = 8.69WANLGAFQNQVKK87 pKa = 8.7STGDD91 pKa = 3.01RR92 pKa = 11.84AGCKK96 pKa = 7.67TQSCYY101 pKa = 11.29ADD103 pKa = 3.05VGYY106 pKa = 11.11RR107 pKa = 11.84LMTDD111 pKa = 2.79QRR113 pKa = 11.84YY114 pKa = 8.52AANAALNEE122 pKa = 3.95VNYY125 pKa = 10.51WMDD128 pKa = 3.06RR129 pKa = 11.84HH130 pKa = 5.83NSNLRR135 pKa = 11.84KK136 pKa = 9.76ALASYY141 pKa = 11.17NSGGNHH147 pKa = 4.25NTASRR152 pKa = 11.84RR153 pKa = 11.84YY154 pKa = 9.01AQDD157 pKa = 3.01VTKK160 pKa = 10.35KK161 pKa = 10.67AKK163 pKa = 10.06YY164 pKa = 9.12LQKK167 pKa = 10.37CVSFAGRR174 pKa = 11.84PVVNKK179 pKa = 9.63PDD181 pKa = 4.07PSVLADD187 pKa = 3.27NTRR190 pKa = 11.84TLKK193 pKa = 10.12RR194 pKa = 11.84LKK196 pKa = 10.34RR197 pKa = 11.84IQQ199 pKa = 3.14

Molecular weight: 21.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

145

0

145

42455

66

1617

292.8

32.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.884 ± 0.25

0.909 ± 0.074

6.539 ± 0.132

6.364 ± 0.227

4.282 ± 0.141

6.774 ± 0.195

1.741 ± 0.101

5.719 ± 0.127

6.277 ± 0.199

7.827 ± 0.169

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.808 ± 0.097

5.123 ± 0.148

3.976 ± 0.143

4.23 ± 0.132

4.949 ± 0.133

6.134 ± 0.138

6.334 ± 0.243

7.064 ± 0.153

1.418 ± 0.091

3.649 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski