Candidatus Doolittlea endobia

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacteriaceae incertae sedis; ant, tsetse, mealybug, aphid, etc. endosymbionts; Candidatus Doolittlea

Average proteome isoelectric point is 7.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 568 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A143WTN3|A0A143WTN3_9ENTR 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ OS=Candidatus Doolittlea endobia OX=1778262 GN=fabZ PE=3 SV=1
MM1 pKa = 7.25FCEE4 pKa = 4.05ACEE7 pKa = 4.26NNDD10 pKa = 3.09NLAASGRR17 pKa = 11.84TDD19 pKa = 4.08TINSLANLTYY29 pKa = 10.18IIVDD33 pKa = 3.95LNVDD37 pKa = 3.61CGSLYY42 pKa = 10.61GYY44 pKa = 10.67SYY46 pKa = 11.82

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A143WS44|A0A143WS44_9ENTR Peptidoglycan D D-transpeptidase MrdA OS=Candidatus Doolittlea endobia OX=1778262 GN=spoVD PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 6.96GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.22NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.55GRR39 pKa = 11.84THH41 pKa = 6.33LTVSFKK47 pKa = 11.16

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

568

0

568

167082

30

1419

294.2

32.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.423 ± 0.097

1.283 ± 0.032

5.174 ± 0.066

5.452 ± 0.104

3.726 ± 0.069

6.846 ± 0.086

2.4 ± 0.048

6.942 ± 0.088

4.991 ± 0.089

10.757 ± 0.155

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.724 ± 0.046

4.172 ± 0.068

3.848 ± 0.062

4.307 ± 0.078

6.377 ± 0.077

6.017 ± 0.079

5.376 ± 0.054

7.116 ± 0.083

1.14 ± 0.045

2.928 ± 0.058
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski