Cherry rusty mottle associated virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Robigovirus

Average proteome isoelectric point is 7.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M9QTU6|M9QTU6_9VIRU Uncharacterized protein OS=Cherry rusty mottle associated virus OX=1312929 PE=4 SV=1
MM1 pKa = 7.63EE2 pKa = 5.22IAHH5 pKa = 7.03GYY7 pKa = 9.99LLDD10 pKa = 4.16ANFEE14 pKa = 4.14RR15 pKa = 11.84TEE17 pKa = 4.1FSLSFPIVVHH27 pKa = 6.15GVPGCGKK34 pKa = 8.15STFVKK39 pKa = 10.44RR40 pKa = 11.84LLDD43 pKa = 3.91CEE45 pKa = 5.06DD46 pKa = 4.21FHH48 pKa = 7.85AQSYY52 pKa = 9.73GVVKK56 pKa = 8.74PTNLAGRR63 pKa = 11.84GVEE66 pKa = 4.54KK67 pKa = 10.53ALQPLQSGFNVLDD80 pKa = 4.47EE81 pKa = 4.39YY82 pKa = 11.6LSGPSYY88 pKa = 11.04EE89 pKa = 5.09GFDD92 pKa = 4.41LLLSDD97 pKa = 5.16PYY99 pKa = 11.53QNFRR103 pKa = 11.84KK104 pKa = 9.6PLTAHH109 pKa = 7.29FINSSTYY116 pKa = 9.19RR117 pKa = 11.84FGHH120 pKa = 5.45SVCKK124 pKa = 10.33YY125 pKa = 9.91LNLLGFEE132 pKa = 4.36INSKK136 pKa = 10.17RR137 pKa = 11.84EE138 pKa = 3.61KK139 pKa = 9.96DD140 pKa = 3.24TEE142 pKa = 4.82LIFGRR147 pKa = 11.84IFEE150 pKa = 4.28GTIKK154 pKa = 11.09GEE156 pKa = 3.99IICFEE161 pKa = 4.4KK162 pKa = 10.64EE163 pKa = 4.11VQEE166 pKa = 4.96LLDD169 pKa = 3.58NHH171 pKa = 5.8SAKK174 pKa = 10.29YY175 pKa = 9.18HH176 pKa = 5.94HH177 pKa = 7.28PCNLRR182 pKa = 11.84GAEE185 pKa = 4.08FEE187 pKa = 4.26HH188 pKa = 6.12VTFISAHH195 pKa = 6.36SDD197 pKa = 3.02LQEE200 pKa = 3.96IVGPDD205 pKa = 3.73LYY207 pKa = 11.37VALTRR212 pKa = 11.84ASEE215 pKa = 4.27SLTILTPP222 pKa = 3.99

Molecular weight: 24.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M9QXN6|M9QXN6_9VIRU Movement protein TGBp3 OS=Cherry rusty mottle associated virus OX=1312929 PE=3 SV=1
MM1 pKa = 7.07MMEE4 pKa = 4.56PSSWTLQGKK13 pKa = 9.32RR14 pKa = 11.84SQRR17 pKa = 11.84KK18 pKa = 8.15RR19 pKa = 11.84RR20 pKa = 11.84PGRR23 pKa = 11.84TLSFLEE29 pKa = 4.4QEE31 pKa = 4.26ASNPKK36 pKa = 10.3DD37 pKa = 3.04RR38 pKa = 11.84TLRR41 pKa = 11.84SSGQEE46 pKa = 3.9EE47 pKa = 4.72EE48 pKa = 4.63EE49 pKa = 4.8LPLTPKK55 pKa = 10.26IPPLVLAEE63 pKa = 4.06TSSAVFKK70 pKa = 10.85IRR72 pKa = 11.84TQLRR76 pKa = 11.84LTLPLTTPSRR86 pKa = 11.84QLRR89 pKa = 11.84LIGSSILRR97 pKa = 11.84FQRR100 pKa = 11.84QKK102 pKa = 11.41YY103 pKa = 9.86LIASLILSGTVIITAPVTKK122 pKa = 10.31QSLLVEE128 pKa = 4.1QSVEE132 pKa = 4.08LNLKK136 pKa = 9.64VLLVLLGATALYY148 pKa = 10.3AVSARR153 pKa = 11.84NMLQQ157 pKa = 2.86

Molecular weight: 17.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

2977

67

2027

425.3

47.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.013 ± 0.535

2.251 ± 0.329

5.039 ± 0.759

6.214 ± 0.798

6.449 ± 1.109

5.778 ± 0.419

2.754 ± 0.596

6.349 ± 0.695

7.054 ± 1.008

10.648 ± 1.916

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.78 ± 0.298

4.232 ± 0.565

3.93 ± 0.566

2.788 ± 0.448

5.173 ± 0.524

9.271 ± 0.577

4.636 ± 1.056

6.013 ± 0.684

0.773 ± 0.157

2.855 ± 0.325
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski