Acidimicrobiia bacterium

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Acidimicrobiia; unclassified Acidimicrobiia

Average proteome isoelectric point is 5.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3762 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L5F832|A0A6L5F832_9ACTN Uncharacterized protein OS=Acidimicrobiia bacterium OX=2080302 GN=GEU79_11390 PE=4 SV=1
MM1 pKa = 7.1SVKK4 pKa = 10.25VLSRR8 pKa = 11.84LSLLLVLSALLIVGVATLGSAAGCGRR34 pKa = 11.84GGNGDD39 pKa = 4.22EE40 pKa = 4.53GWAGCTPEE48 pKa = 5.43KK49 pKa = 10.68IPDD52 pKa = 3.62RR53 pKa = 11.84NYY55 pKa = 11.19DD56 pKa = 3.93DD57 pKa = 4.42PQEE60 pKa = 4.38DD61 pKa = 3.69PRR63 pKa = 11.84GNPISEE69 pKa = 4.33DD70 pKa = 4.01EE71 pKa = 4.31LWQMYY76 pKa = 10.14CVDD79 pKa = 5.15RR80 pKa = 11.84YY81 pKa = 10.35PDD83 pKa = 3.59YY84 pKa = 11.62DD85 pKa = 4.02GDD87 pKa = 4.26TEE89 pKa = 4.29TGPWDD94 pKa = 3.9NDD96 pKa = 3.72PEE98 pKa = 4.43SLNDD102 pKa = 3.98FYY104 pKa = 11.62YY105 pKa = 9.62DD106 pKa = 3.48TRR108 pKa = 11.84PIPDD112 pKa = 5.42FILDD116 pKa = 4.87DD117 pKa = 4.04MNLDD121 pKa = 3.49PTGVYY126 pKa = 8.33IWVWAVCGGPDD137 pKa = 3.19WEE139 pKa = 4.52RR140 pKa = 11.84NRR142 pKa = 11.84DD143 pKa = 3.49QFGYY147 pKa = 10.42GPQGANFANWW157 pKa = 2.97

Molecular weight: 17.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L5F3N4|A0A6L5F3N4_9ACTN DUF1989 domain-containing protein OS=Acidimicrobiia bacterium OX=2080302 GN=GEU79_03310 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.37RR3 pKa = 11.84TYY5 pKa = 9.3QPKK8 pKa = 8.17VRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.22RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84HH20 pKa = 5.9RR21 pKa = 11.84MQTRR25 pKa = 11.84AGRR28 pKa = 11.84NVLKK32 pKa = 10.5SRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.74GRR39 pKa = 11.84KK40 pKa = 8.82RR41 pKa = 11.84LAAA44 pKa = 4.42

Molecular weight: 5.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3762

0

3762

1092149

19

2505

290.3

31.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.397 ± 0.047

0.66 ± 0.013

6.751 ± 0.048

6.797 ± 0.038

3.262 ± 0.026

9.129 ± 0.038

2.145 ± 0.018

5.0 ± 0.029

1.98 ± 0.022

9.774 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.248 ± 0.016

2.307 ± 0.023

5.375 ± 0.032

2.615 ± 0.019

6.897 ± 0.044

5.962 ± 0.024

6.25 ± 0.034

8.928 ± 0.037

1.498 ± 0.018

2.025 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski