Sphingobium sp. RAC03

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium; unclassified Sphingobium

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4109 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B3MTR0|A0A1B3MTR0_9SPHN Bacterial regulatory s tetR family protein OS=Sphingobium sp. RAC03 OX=1843368 GN=BSY17_2335 PE=4 SV=1
MM1 pKa = 8.13DD2 pKa = 3.97FTGGAGDD9 pKa = 5.82DD10 pKa = 4.37IYY12 pKa = 11.26TGDD15 pKa = 4.2NSDD18 pKa = 4.14EE19 pKa = 4.42YY20 pKa = 11.01ISGLGGNDD28 pKa = 3.01WLSGGEE34 pKa = 4.11GADD37 pKa = 3.92NIAGGAGADD46 pKa = 3.87RR47 pKa = 11.84LNGGDD52 pKa = 3.54GSDD55 pKa = 3.36VLFSFGRR62 pKa = 11.84TTNMMAPYY70 pKa = 9.6FPLLAGVVSDD80 pKa = 6.4DD81 pKa = 3.47IFADD85 pKa = 3.22VDD87 pKa = 3.97TLIGGAGDD95 pKa = 4.03DD96 pKa = 4.45FFFAGYY102 pKa = 10.04GDD104 pKa = 4.95HH105 pKa = 7.46IDD107 pKa = 3.78GGSYY111 pKa = 11.0DD112 pKa = 3.42SFGNRR117 pKa = 11.84LFISFQGATSGVVADD132 pKa = 6.13FRR134 pKa = 11.84PLQNGEE140 pKa = 4.37SITIGGGIISNIQNIGFLEE159 pKa = 4.11GRR161 pKa = 11.84EE162 pKa = 3.9YY163 pKa = 11.45DD164 pKa = 4.73DD165 pKa = 5.92FIASIDD171 pKa = 3.47TYY173 pKa = 10.32YY174 pKa = 10.57PSGADD179 pKa = 2.77IFGRR183 pKa = 11.84GGNDD187 pKa = 3.4TIIADD192 pKa = 4.39YY193 pKa = 10.58YY194 pKa = 10.4SGWGGAIWGGDD205 pKa = 3.22GDD207 pKa = 4.4DD208 pKa = 4.06TVDD211 pKa = 3.47ATGAQYY217 pKa = 10.78GARR220 pKa = 11.84VYY222 pKa = 11.18GEE224 pKa = 4.46AGNDD228 pKa = 3.82IIRR231 pKa = 11.84MNYY234 pKa = 7.29GTADD238 pKa = 3.59GGDD241 pKa = 3.89GDD243 pKa = 5.19DD244 pKa = 4.32IIYY247 pKa = 10.56GGSAFGGAGNDD258 pKa = 3.33RR259 pKa = 11.84LIDD262 pKa = 3.73VMSGDD267 pKa = 4.21GGDD270 pKa = 3.82GNDD273 pKa = 3.83IITLNFSYY281 pKa = 10.55YY282 pKa = 10.72GNGVATGGRR291 pKa = 11.84GDD293 pKa = 3.86DD294 pKa = 4.71LIRR297 pKa = 11.84GTDD300 pKa = 3.52YY301 pKa = 11.56GSVLVGGEE309 pKa = 4.09GADD312 pKa = 3.63EE313 pKa = 4.41LFGGAGADD321 pKa = 4.17LIYY324 pKa = 10.7TGGRR328 pKa = 11.84NEE330 pKa = 5.18DD331 pKa = 3.35GSALADD337 pKa = 3.36VGVEE341 pKa = 3.85KK342 pKa = 11.08DD343 pKa = 3.98FVSAGAGADD352 pKa = 3.96TIWAGVGDD360 pKa = 4.83DD361 pKa = 3.83VDD363 pKa = 5.12GGDD366 pKa = 4.5GADD369 pKa = 3.06TLYY372 pKa = 11.19YY373 pKa = 10.59SFGGATTGVDD383 pKa = 2.89ISTHH387 pKa = 4.67QFLGATARR395 pKa = 11.84VIGGGTIISIEE406 pKa = 4.09SLAEE410 pKa = 3.75LRR412 pKa = 11.84ASSFDD417 pKa = 3.53DD418 pKa = 4.27RR419 pKa = 11.84IVATTQDD426 pKa = 3.5VRR428 pKa = 11.84LSIYY432 pKa = 10.48GGAGNDD438 pKa = 3.42VVTSSGSSITFRR450 pKa = 11.84GEE452 pKa = 3.59EE453 pKa = 4.09GDD455 pKa = 4.07DD456 pKa = 3.4RR457 pKa = 11.84LISGEE462 pKa = 4.0AADD465 pKa = 4.02MFVGGFGTDD474 pKa = 3.25TIDD477 pKa = 3.4YY478 pKa = 10.37SLYY481 pKa = 8.9TNGVTVTLGLSGAIGIGGGGDD502 pKa = 3.43SLISVEE508 pKa = 4.3NVVGSAFSDD517 pKa = 4.11DD518 pKa = 3.45IQGNEE523 pKa = 4.29LANMLEE529 pKa = 4.46GGAGNDD535 pKa = 4.28TIHH538 pKa = 6.96GGAGDD543 pKa = 4.43DD544 pKa = 3.67RR545 pKa = 11.84LLPGTGHH552 pKa = 6.81NIVDD556 pKa = 4.08GGDD559 pKa = 3.65GFDD562 pKa = 3.6TLVLTGGVASYY573 pKa = 11.23NYY575 pKa = 9.56LVSGASTFVIGEE587 pKa = 4.17EE588 pKa = 4.2GASRR592 pKa = 11.84IVNVEE597 pKa = 3.7HH598 pKa = 6.59VAFANGALGSADD610 pKa = 3.46LAGSLSAFDD619 pKa = 3.73GLRR622 pKa = 11.84YY623 pKa = 8.98IAGYY627 pKa = 10.23GDD629 pKa = 5.42LIAAFGSDD637 pKa = 3.51ADD639 pKa = 3.95SATAHH644 pKa = 5.63YY645 pKa = 9.48VASGFAEE652 pKa = 4.24GRR654 pKa = 11.84DD655 pKa = 3.32AKK657 pKa = 10.91EE658 pKa = 3.92FDD660 pKa = 3.67VLDD663 pKa = 4.37YY664 pKa = 11.01IAGYY668 pKa = 10.5DD669 pKa = 3.71DD670 pKa = 6.12LIAAFGTDD678 pKa = 3.18TQAATSHH685 pKa = 6.3YY686 pKa = 9.32IDD688 pKa = 3.91GGFVEE693 pKa = 5.02GRR695 pKa = 11.84SDD697 pKa = 3.7ALFDD701 pKa = 3.64GLQYY705 pKa = 10.23IASYY709 pKa = 10.92GDD711 pKa = 3.93LIEE714 pKa = 5.77AFGTDD719 pKa = 2.83ADD721 pKa = 3.49AGARR725 pKa = 11.84HH726 pKa = 6.8FIQGGRR732 pKa = 11.84DD733 pKa = 3.2EE734 pKa = 5.16GRR736 pKa = 11.84SDD738 pKa = 4.61DD739 pKa = 5.51LFDD742 pKa = 3.91GLQYY746 pKa = 10.2VASYY750 pKa = 10.81GDD752 pKa = 4.23LIGAIGTDD760 pKa = 3.27VEE762 pKa = 4.28AAAEE766 pKa = 4.05HH767 pKa = 6.67FIKK770 pKa = 10.71NGYY773 pKa = 9.35GEE775 pKa = 4.86GRR777 pKa = 11.84MADD780 pKa = 3.72DD781 pKa = 4.28FDD783 pKa = 3.83GLRR786 pKa = 11.84YY787 pKa = 9.29IASNADD793 pKa = 3.99LIVALGDD800 pKa = 4.28DD801 pKa = 4.1DD802 pKa = 5.53DD803 pKa = 4.39AAARR807 pKa = 11.84HH808 pKa = 6.02YY809 pKa = 10.69ILAGQQEE816 pKa = 4.5GRR818 pKa = 11.84DD819 pKa = 3.52VDD821 pKa = 4.41GFDD824 pKa = 3.54ALAYY828 pKa = 10.06AAANPDD834 pKa = 3.09LAAAFGSDD842 pKa = 2.95VDD844 pKa = 4.76ALTEE848 pKa = 4.16HH849 pKa = 6.83YY850 pKa = 10.33IDD852 pKa = 3.05TGYY855 pKa = 10.87FEE857 pKa = 5.13HH858 pKa = 7.26RR859 pKa = 11.84VVAPGADD866 pKa = 2.83MFMAGG871 pKa = 4.09

Molecular weight: 88.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B3MRB7|A0A1B3MRB7_9SPHN Uncharacterized protein OS=Sphingobium sp. RAC03 OX=1843368 GN=BSY17_494 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.33RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 5.19GYY18 pKa = 7.59RR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84NIIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4109

0

4109

1290787

29

2504

314.1

33.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.085 ± 0.057

0.819 ± 0.012

6.323 ± 0.033

5.002 ± 0.038

3.485 ± 0.023

8.8 ± 0.054

2.044 ± 0.02

5.295 ± 0.023

3.06 ± 0.029

9.797 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.643 ± 0.018

2.564 ± 0.025

5.307 ± 0.03

3.357 ± 0.024

7.191 ± 0.034

5.224 ± 0.027

5.33 ± 0.026

6.956 ± 0.024

1.442 ± 0.014

2.277 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski