Amino acid dipepetide frequency for Caulobacter phage Kronos

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.785AlaAla: 22.785 ± 2.202
1.559AlaCys: 1.559 ± 0.442
7.347AlaAsp: 7.347 ± 0.742
7.867AlaGlu: 7.867 ± 0.997
5.344AlaPhe: 5.344 ± 0.676
11.133AlaGly: 11.133 ± 1.273
2.969AlaHis: 2.969 ± 0.412
4.973AlaIle: 4.973 ± 0.688
6.68AlaLys: 6.68 ± 1.033
11.133AlaLeu: 11.133 ± 0.964
4.973AlaMet: 4.973 ± 0.701
4.601AlaAsn: 4.601 ± 0.659
7.051AlaPro: 7.051 ± 0.772
5.418AlaGln: 5.418 ± 0.746
7.644AlaArg: 7.644 ± 0.942
7.719AlaSer: 7.719 ± 0.661
7.867AlaThr: 7.867 ± 0.976
10.687AlaVal: 10.687 ± 1.165
2.375AlaTrp: 2.375 ± 0.519
2.969AlaTyr: 2.969 ± 0.529
0.0AlaXaa: 0.0 ± 0.0
Cys
1.187CysAla: 1.187 ± 0.346
0.371CysCys: 0.371 ± 0.158
0.445CysAsp: 0.445 ± 0.206
0.445CysGlu: 0.445 ± 0.234
0.148CysPhe: 0.148 ± 0.115
1.113CysGly: 1.113 ± 0.261
0.297CysHis: 0.297 ± 0.199
0.371CysIle: 0.371 ± 0.185
0.668CysLys: 0.668 ± 0.258
0.668CysLeu: 0.668 ± 0.215
0.148CysMet: 0.148 ± 0.116
0.074CysAsn: 0.074 ± 0.077
0.668CysPro: 0.668 ± 0.26
0.52CysGln: 0.52 ± 0.246
0.816CysArg: 0.816 ± 0.221
0.297CysSer: 0.297 ± 0.164
0.594CysThr: 0.594 ± 0.235
0.52CysVal: 0.52 ± 0.221
0.0CysTrp: 0.0 ± 0.0
0.594CysTyr: 0.594 ± 0.206
0.0CysXaa: 0.0 ± 0.0
Asp
8.164AspAla: 8.164 ± 0.691
0.594AspCys: 0.594 ± 0.231
2.523AspAsp: 2.523 ± 0.359
3.488AspGlu: 3.488 ± 0.646
2.227AspPhe: 2.227 ± 0.429
6.086AspGly: 6.086 ± 0.64
1.336AspHis: 1.336 ± 0.332
1.633AspIle: 1.633 ± 0.441
2.078AspLys: 2.078 ± 0.579
3.785AspLeu: 3.785 ± 0.542
0.52AspMet: 0.52 ± 0.202
0.965AspAsn: 0.965 ± 0.283
3.711AspPro: 3.711 ± 0.68
2.301AspGln: 2.301 ± 0.371
2.82AspArg: 2.82 ± 0.466
2.523AspSer: 2.523 ± 0.419
3.414AspThr: 3.414 ± 0.687
4.082AspVal: 4.082 ± 0.61
0.742AspTrp: 0.742 ± 0.202
1.484AspTyr: 1.484 ± 0.381
0.0AspXaa: 0.0 ± 0.0
Glu
8.758GluAla: 8.758 ± 1.312
0.297GluCys: 0.297 ± 0.152
2.449GluAsp: 2.449 ± 0.484
3.191GluGlu: 3.191 ± 0.595
1.707GluPhe: 1.707 ± 0.367
3.562GluGly: 3.562 ± 0.586
1.41GluHis: 1.41 ± 0.409
1.707GluIle: 1.707 ± 0.413
2.152GluLys: 2.152 ± 0.517
5.121GluLeu: 5.121 ± 0.671
1.707GluMet: 1.707 ± 0.385
1.559GluAsn: 1.559 ± 0.369
3.34GluPro: 3.34 ± 0.542
2.449GluGln: 2.449 ± 0.402
4.23GluArg: 4.23 ± 0.638
1.484GluSer: 1.484 ± 0.329
3.859GluThr: 3.859 ± 0.485
4.379GluVal: 4.379 ± 0.565
0.52GluTrp: 0.52 ± 0.189
1.336GluTyr: 1.336 ± 0.328
0.0GluXaa: 0.0 ± 0.0
Phe
4.23PheAla: 4.23 ± 0.599
0.371PheCys: 0.371 ± 0.172
2.078PheAsp: 2.078 ± 0.464
1.262PheGlu: 1.262 ± 0.268
0.594PhePhe: 0.594 ± 0.225
3.637PheGly: 3.637 ± 0.514
0.52PheHis: 0.52 ± 0.204
1.113PheIle: 1.113 ± 0.266
1.484PheLys: 1.484 ± 0.325
2.746PheLeu: 2.746 ± 0.463
1.41PheMet: 1.41 ± 0.345
1.187PheAsn: 1.187 ± 0.339
1.113PhePro: 1.113 ± 0.326
1.262PheGln: 1.262 ± 0.31
1.855PheArg: 1.855 ± 0.455
1.707PheSer: 1.707 ± 0.401
1.93PheThr: 1.93 ± 0.345
2.672PheVal: 2.672 ± 0.322
0.445PheTrp: 0.445 ± 0.229
0.594PheTyr: 0.594 ± 0.248
0.0PheXaa: 0.0 ± 0.0
Gly
10.094GlyAla: 10.094 ± 1.209
0.594GlyCys: 0.594 ± 0.236
5.269GlyAsp: 5.269 ± 0.63
4.305GlyGlu: 4.305 ± 0.627
3.934GlyPhe: 3.934 ± 0.575
8.98GlyGly: 8.98 ± 0.955
1.262GlyHis: 1.262 ± 0.344
2.523GlyIle: 2.523 ± 0.4
3.785GlyLys: 3.785 ± 0.504
7.199GlyLeu: 7.199 ± 0.887
1.781GlyMet: 1.781 ± 0.321
3.191GlyAsn: 3.191 ± 0.388
3.711GlyPro: 3.711 ± 0.631
4.008GlyGln: 4.008 ± 0.633
5.344GlyArg: 5.344 ± 0.627
4.601GlySer: 4.601 ± 0.787
6.086GlyThr: 6.086 ± 0.971
6.234GlyVal: 6.234 ± 0.84
2.598GlyTrp: 2.598 ± 0.433
2.078GlyTyr: 2.078 ± 0.494
0.0GlyXaa: 0.0 ± 0.0
His
2.078HisAla: 2.078 ± 0.391
0.0HisCys: 0.0 ± 0.0
1.41HisAsp: 1.41 ± 0.298
1.262HisGlu: 1.262 ± 0.376
0.52HisPhe: 0.52 ± 0.166
1.781HisGly: 1.781 ± 0.453
0.371HisHis: 0.371 ± 0.228
0.297HisIle: 0.297 ± 0.16
0.965HisLys: 0.965 ± 0.245
2.227HisLeu: 2.227 ± 0.507
0.445HisMet: 0.445 ± 0.232
0.445HisAsn: 0.445 ± 0.205
1.484HisPro: 1.484 ± 0.286
0.742HisGln: 0.742 ± 0.193
1.559HisArg: 1.559 ± 0.461
0.742HisSer: 0.742 ± 0.228
0.891HisThr: 0.891 ± 0.264
1.336HisVal: 1.336 ± 0.332
0.223HisTrp: 0.223 ± 0.139
0.445HisTyr: 0.445 ± 0.144
0.0HisXaa: 0.0 ± 0.0
Ile
4.75IleAla: 4.75 ± 0.628
0.223IleCys: 0.223 ± 0.114
2.078IleAsp: 2.078 ± 0.426
1.781IleGlu: 1.781 ± 0.34
0.742IlePhe: 0.742 ± 0.289
3.414IleGly: 3.414 ± 0.867
0.891IleHis: 0.891 ± 0.256
1.855IleIle: 1.855 ± 0.364
1.633IleLys: 1.633 ± 0.34
2.078IleLeu: 2.078 ± 0.45
0.891IleMet: 0.891 ± 0.242
0.594IleAsn: 0.594 ± 0.283
2.078IlePro: 2.078 ± 0.487
1.113IleGln: 1.113 ± 0.249
2.598IleArg: 2.598 ± 0.497
1.41IleSer: 1.41 ± 0.379
2.375IleThr: 2.375 ± 0.469
2.746IleVal: 2.746 ± 0.379
0.445IleTrp: 0.445 ± 0.177
0.223IleTyr: 0.223 ± 0.16
0.0IleXaa: 0.0 ± 0.0
Lys
8.015LysAla: 8.015 ± 1.043
0.297LysCys: 0.297 ± 0.151
1.93LysAsp: 1.93 ± 0.435
1.93LysGlu: 1.93 ± 0.419
0.891LysPhe: 0.891 ± 0.264
3.562LysGly: 3.562 ± 0.442
1.039LysHis: 1.039 ± 0.304
1.336LysIle: 1.336 ± 0.385
1.262LysLys: 1.262 ± 0.327
3.562LysLeu: 3.562 ± 0.581
1.41LysMet: 1.41 ± 0.307
1.41LysAsn: 1.41 ± 0.298
3.191LysPro: 3.191 ± 0.584
1.187LysGln: 1.187 ± 0.213
2.969LysArg: 2.969 ± 0.434
1.781LysSer: 1.781 ± 0.306
2.078LysThr: 2.078 ± 0.47
2.746LysVal: 2.746 ± 0.484
0.891LysTrp: 0.891 ± 0.313
0.594LysTyr: 0.594 ± 0.263
0.0LysXaa: 0.0 ± 0.0
Leu
13.582LeuAla: 13.582 ± 1.26
1.187LeuCys: 1.187 ± 0.361
5.047LeuAsp: 5.047 ± 0.539
5.64LeuGlu: 5.64 ± 0.627
2.894LeuPhe: 2.894 ± 0.523
7.125LeuGly: 7.125 ± 0.901
2.152LeuHis: 2.152 ± 0.447
2.078LeuIle: 2.078 ± 0.429
3.711LeuLys: 3.711 ± 0.542
6.754LeuLeu: 6.754 ± 0.881
1.93LeuMet: 1.93 ± 0.424
2.078LeuAsn: 2.078 ± 0.444
4.305LeuPro: 4.305 ± 0.789
2.672LeuGln: 2.672 ± 0.45
6.308LeuArg: 6.308 ± 0.771
3.785LeuSer: 3.785 ± 0.587
4.75LeuThr: 4.75 ± 0.6
4.75LeuVal: 4.75 ± 0.559
1.559LeuTrp: 1.559 ± 0.421
1.336LeuTyr: 1.336 ± 0.306
0.0LeuXaa: 0.0 ± 0.0
Met
3.488MetAla: 3.488 ± 0.553
0.371MetCys: 0.371 ± 0.174
1.707MetAsp: 1.707 ± 0.413
1.113MetGlu: 1.113 ± 0.304
0.52MetPhe: 0.52 ± 0.182
1.93MetGly: 1.93 ± 0.425
0.297MetHis: 0.297 ± 0.181
0.891MetIle: 0.891 ± 0.232
1.41MetLys: 1.41 ± 0.409
2.078MetLeu: 2.078 ± 0.461
0.371MetMet: 0.371 ± 0.209
1.113MetAsn: 1.113 ± 0.212
2.449MetPro: 2.449 ± 0.423
0.965MetGln: 0.965 ± 0.234
2.078MetArg: 2.078 ± 0.472
1.93MetSer: 1.93 ± 0.366
2.449MetThr: 2.449 ± 0.49
1.41MetVal: 1.41 ± 0.36
0.445MetTrp: 0.445 ± 0.195
0.074MetTyr: 0.074 ± 0.083
0.0MetXaa: 0.0 ± 0.0
Asn
4.601AsnAla: 4.601 ± 0.592
0.297AsnCys: 0.297 ± 0.179
1.262AsnAsp: 1.262 ± 0.26
1.41AsnGlu: 1.41 ± 0.338
0.52AsnPhe: 0.52 ± 0.201
2.969AsnGly: 2.969 ± 0.651
0.297AsnHis: 0.297 ± 0.142
1.336AsnIle: 1.336 ± 0.465
0.445AsnLys: 0.445 ± 0.17
1.633AsnLeu: 1.633 ± 0.258
0.742AsnMet: 0.742 ± 0.197
0.742AsnAsn: 0.742 ± 0.246
2.672AsnPro: 2.672 ± 0.509
0.965AsnGln: 0.965 ± 0.282
2.004AsnArg: 2.004 ± 0.34
1.113AsnSer: 1.113 ± 0.227
2.227AsnThr: 2.227 ± 0.395
2.375AsnVal: 2.375 ± 0.416
0.816AsnTrp: 0.816 ± 0.253
0.52AsnTyr: 0.52 ± 0.233
0.0AsnXaa: 0.0 ± 0.0
Pro
8.535ProAla: 8.535 ± 0.817
0.816ProCys: 0.816 ± 0.256
3.785ProAsp: 3.785 ± 0.596
4.156ProGlu: 4.156 ± 0.653
1.93ProPhe: 1.93 ± 0.492
5.195ProGly: 5.195 ± 0.648
0.742ProHis: 0.742 ± 0.29
1.707ProIle: 1.707 ± 0.35
2.598ProLys: 2.598 ± 0.56
4.379ProLeu: 4.379 ± 0.624
1.187ProMet: 1.187 ± 0.288
2.375ProAsn: 2.375 ± 0.314
4.23ProPro: 4.23 ± 0.821
1.484ProGln: 1.484 ± 0.359
3.34ProArg: 3.34 ± 0.563
2.746ProSer: 2.746 ± 0.485
3.785ProThr: 3.785 ± 0.788
4.75ProVal: 4.75 ± 0.596
1.336ProTrp: 1.336 ± 0.354
0.742ProTyr: 0.742 ± 0.194
0.0ProXaa: 0.0 ± 0.0
Gln
5.715GlnAla: 5.715 ± 0.64
0.148GlnCys: 0.148 ± 0.1
1.41GlnAsp: 1.41 ± 0.295
1.336GlnGlu: 1.336 ± 0.347
1.336GlnPhe: 1.336 ± 0.325
2.82GlnGly: 2.82 ± 0.493
0.965GlnHis: 0.965 ± 0.243
1.187GlnIle: 1.187 ± 0.243
1.187GlnLys: 1.187 ± 0.317
4.379GlnLeu: 4.379 ± 0.509
0.668GlnMet: 0.668 ± 0.224
0.965GlnAsn: 0.965 ± 0.294
1.855GlnPro: 1.855 ± 0.375
1.633GlnGln: 1.633 ± 0.429
2.82GlnArg: 2.82 ± 0.5
1.559GlnSer: 1.559 ± 0.423
2.152GlnThr: 2.152 ± 0.292
2.523GlnVal: 2.523 ± 0.434
0.297GlnTrp: 0.297 ± 0.148
0.594GlnTyr: 0.594 ± 0.27
0.0GlnXaa: 0.0 ± 0.0
Arg
8.683ArgAla: 8.683 ± 0.798
0.52ArgCys: 0.52 ± 0.24
2.375ArgAsp: 2.375 ± 0.476
4.898ArgGlu: 4.898 ± 0.812
2.523ArgPhe: 2.523 ± 0.458
4.156ArgGly: 4.156 ± 0.596
1.039ArgHis: 1.039 ± 0.313
2.894ArgIle: 2.894 ± 0.394
3.117ArgLys: 3.117 ± 0.552
6.531ArgLeu: 6.531 ± 0.664
2.449ArgMet: 2.449 ± 0.491
2.152ArgAsn: 2.152 ± 0.421
3.191ArgPro: 3.191 ± 0.539
2.523ArgGln: 2.523 ± 0.532
5.269ArgArg: 5.269 ± 0.828
2.375ArgSer: 2.375 ± 0.494
3.859ArgThr: 3.859 ± 0.545
4.305ArgVal: 4.305 ± 0.557
0.816ArgTrp: 0.816 ± 0.223
1.484ArgTyr: 1.484 ± 0.343
0.0ArgXaa: 0.0 ± 0.0
Ser
5.789SerAla: 5.789 ± 0.633
0.223SerCys: 0.223 ± 0.127
2.746SerAsp: 2.746 ± 0.45
2.227SerGlu: 2.227 ± 0.411
2.152SerPhe: 2.152 ± 0.405
5.195SerGly: 5.195 ± 0.654
0.668SerHis: 0.668 ± 0.24
1.262SerIle: 1.262 ± 0.295
1.559SerLys: 1.559 ± 0.4
5.789SerLeu: 5.789 ± 0.613
1.559SerMet: 1.559 ± 0.343
1.039SerAsn: 1.039 ± 0.261
3.414SerPro: 3.414 ± 0.44
0.891SerGln: 0.891 ± 0.248
2.672SerArg: 2.672 ± 0.397
1.93SerSer: 1.93 ± 0.423
3.562SerThr: 3.562 ± 0.556
4.082SerVal: 4.082 ± 0.546
0.594SerTrp: 0.594 ± 0.233
0.223SerTyr: 0.223 ± 0.154
0.0SerXaa: 0.0 ± 0.0
Thr
7.793ThrAla: 7.793 ± 0.864
1.113ThrCys: 1.113 ± 0.289
3.859ThrAsp: 3.859 ± 0.543
2.598ThrGlu: 2.598 ± 0.514
1.93ThrPhe: 1.93 ± 0.403
6.902ThrGly: 6.902 ± 0.986
1.187ThrHis: 1.187 ± 0.262
3.191ThrIle: 3.191 ± 0.49
2.894ThrLys: 2.894 ± 0.447
5.863ThrLeu: 5.863 ± 0.682
2.004ThrMet: 2.004 ± 0.368
1.336ThrAsn: 1.336 ± 0.348
4.23ThrPro: 4.23 ± 0.498
1.633ThrGln: 1.633 ± 0.314
3.043ThrArg: 3.043 ± 0.545
3.266ThrSer: 3.266 ± 0.562
4.379ThrThr: 4.379 ± 0.917
4.676ThrVal: 4.676 ± 0.58
0.742ThrTrp: 0.742 ± 0.193
1.484ThrTyr: 1.484 ± 0.321
0.0ThrXaa: 0.0 ± 0.0
Val
10.168ValAla: 10.168 ± 0.959
0.594ValCys: 0.594 ± 0.262
4.379ValAsp: 4.379 ± 0.724
4.527ValGlu: 4.527 ± 0.673
1.855ValPhe: 1.855 ± 0.496
5.269ValGly: 5.269 ± 0.652
1.262ValHis: 1.262 ± 0.282
2.82ValIle: 2.82 ± 0.413
3.637ValLys: 3.637 ± 0.565
4.973ValLeu: 4.973 ± 0.583
1.855ValMet: 1.855 ± 0.374
2.152ValAsn: 2.152 ± 0.496
3.934ValPro: 3.934 ± 0.614
2.449ValGln: 2.449 ± 0.362
4.527ValArg: 4.527 ± 0.639
4.527ValSer: 4.527 ± 0.709
5.195ValThr: 5.195 ± 0.627
6.531ValVal: 6.531 ± 0.682
1.262ValTrp: 1.262 ± 0.411
2.004ValTyr: 2.004 ± 0.602
0.0ValXaa: 0.0 ± 0.0
Trp
2.523TrpAla: 2.523 ± 0.392
0.297TrpCys: 0.297 ± 0.179
0.816TrpAsp: 0.816 ± 0.202
0.668TrpGlu: 0.668 ± 0.235
0.074TrpPhe: 0.074 ± 0.08
0.816TrpGly: 0.816 ± 0.293
0.297TrpHis: 0.297 ± 0.15
0.223TrpIle: 0.223 ± 0.136
0.594TrpLys: 0.594 ± 0.267
1.336TrpLeu: 1.336 ± 0.292
0.52TrpMet: 0.52 ± 0.233
0.594TrpAsn: 0.594 ± 0.22
1.41TrpPro: 1.41 ± 0.418
0.742TrpGln: 0.742 ± 0.211
1.41TrpArg: 1.41 ± 0.343
1.187TrpSer: 1.187 ± 0.263
1.336TrpThr: 1.336 ± 0.339
1.41TrpVal: 1.41 ± 0.413
0.594TrpTrp: 0.594 ± 0.206
0.223TrpTyr: 0.223 ± 0.14
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.152TyrAla: 2.152 ± 0.516
0.074TyrCys: 0.074 ± 0.08
1.559TyrAsp: 1.559 ± 0.342
1.113TyrGlu: 1.113 ± 0.33
0.371TyrPhe: 0.371 ± 0.139
1.93TyrGly: 1.93 ± 0.352
0.223TyrHis: 0.223 ± 0.113
0.52TyrIle: 0.52 ± 0.205
0.297TyrLys: 0.297 ± 0.14
1.41TyrLeu: 1.41 ± 0.333
0.445TyrMet: 0.445 ± 0.176
0.371TyrAsn: 0.371 ± 0.207
1.781TyrPro: 1.781 ± 0.423
0.594TyrGln: 0.594 ± 0.195
1.855TyrArg: 1.855 ± 0.361
0.891TyrSer: 0.891 ± 0.233
1.336TyrThr: 1.336 ± 0.32
1.707TyrVal: 1.707 ± 0.374
0.371TyrTrp: 0.371 ± 0.169
0.371TyrTyr: 0.371 ± 0.203
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (13475 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski