Escherichia virus mEp460_4F5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A653FRG7|A0A653FRG7_9CAUD Ad1 adapter protein OS=Escherichia virus mEp460_4F5 OX=2686058 PE=4 SV=1
MM1 pKa = 8.0ADD3 pKa = 3.13PMNRR7 pKa = 11.84HH8 pKa = 3.81TQIRR12 pKa = 11.84QVVLARR18 pKa = 11.84LRR20 pKa = 11.84EE21 pKa = 4.03QCGDD25 pKa = 3.08SATFFDD31 pKa = 4.77GLPAFVDD38 pKa = 3.93AQEE41 pKa = 4.48LPAVVVWLSDD51 pKa = 3.17AQYY54 pKa = 8.79TGKK57 pKa = 7.91MTDD60 pKa = 3.19EE61 pKa = 5.38DD62 pKa = 3.58DD63 pKa = 3.67WQAVLHH69 pKa = 5.99IAVFIRR75 pKa = 11.84AQAPDD80 pKa = 3.93SEE82 pKa = 5.25LDD84 pKa = 2.98MWMEE88 pKa = 4.06STIFPALNDD97 pKa = 3.5VPALSGLIDD106 pKa = 3.52TLIPLGFNYY115 pKa = 9.81QRR117 pKa = 11.84DD118 pKa = 3.81NEE120 pKa = 4.26MATWAMTEE128 pKa = 3.32ITYY131 pKa = 10.58QITYY135 pKa = 9.38TNN137 pKa = 3.43

Molecular weight: 15.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A653FRM3|A0A653FRM3_9CAUD Uncharacterized protein OS=Escherichia virus mEp460_4F5 OX=2686058 PE=4 SV=1
MM1 pKa = 6.97QRR3 pKa = 11.84FGRR6 pKa = 11.84RR7 pKa = 11.84IDD9 pKa = 3.87LRR11 pKa = 11.84VKK13 pKa = 9.79TGAEE17 pKa = 4.31AIRR20 pKa = 11.84ALATQLPVFRR30 pKa = 11.84QKK32 pKa = 11.14LSDD35 pKa = 2.4GWYY38 pKa = 8.28QVRR41 pKa = 11.84IAGRR45 pKa = 11.84DD46 pKa = 3.38VSTSGLTAQLHH57 pKa = 4.92EE58 pKa = 4.58TLPDD62 pKa = 3.34GAVIHH67 pKa = 6.17IVPRR71 pKa = 11.84VAGAKK76 pKa = 9.96SGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKK136 pKa = 10.24ARR138 pKa = 11.84TPRR141 pKa = 11.84TQTTDD146 pKa = 2.73NGKK149 pKa = 9.57QNTYY153 pKa = 10.29FSSLDD158 pKa = 3.41NMVAQGNVLPVLYY171 pKa = 10.98GEE173 pKa = 4.33MRR175 pKa = 11.84VGSRR179 pKa = 11.84VVSQEE184 pKa = 3.03ISTADD189 pKa = 3.49EE190 pKa = 4.43GDD192 pKa = 3.44GGQVVVIGRR201 pKa = 3.65

Molecular weight: 20.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

13113

40

1160

215.0

23.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.411 ± 0.574

1.243 ± 0.158

5.925 ± 0.215

6.383 ± 0.295

3.508 ± 0.231

7.1 ± 0.26

1.746 ± 0.165

5.239 ± 0.23

5.483 ± 0.293

7.847 ± 0.297

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.646 ± 0.191

4.354 ± 0.297

4.057 ± 0.325

4.553 ± 0.501

6.429 ± 0.371

6.49 ± 0.253

5.964 ± 0.388

6.879 ± 0.358

1.914 ± 0.194

2.829 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski