Plasmodiophora brassicae (Clubroot disease)

Taxonomy: cellular organisms; Eukaryota; Sar; Rhizaria; Endomyxa; Phytomyxea; Plasmodiophorida; Plasmodiophoridae; Plasmodiophora

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9720 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G4ISR1|A0A0G4ISR1_PLABS Uncharacterized protein OS=Plasmodiophora brassicae OX=37360 GN=PBRA_006245 PE=4 SV=1
MM1 pKa = 7.61YY2 pKa = 7.89DD3 pKa = 3.26TPEE6 pKa = 4.38FGDD9 pKa = 3.58DD10 pKa = 3.91RR11 pKa = 11.84EE12 pKa = 4.65DD13 pKa = 3.46VSSGDD18 pKa = 3.58DD19 pKa = 3.59SVDD22 pKa = 3.24EE23 pKa = 4.31RR24 pKa = 11.84AVDD27 pKa = 4.17CGSDD31 pKa = 3.77DD32 pKa = 5.55DD33 pKa = 5.97DD34 pKa = 5.05SEE36 pKa = 4.94NYY38 pKa = 10.87GDD40 pKa = 4.33FEE42 pKa = 4.89VVGPADD48 pKa = 3.55QLTPTNPALAVVIEE62 pKa = 4.16AAEE65 pKa = 3.95QGDD68 pKa = 3.84VDD70 pKa = 3.97RR71 pKa = 11.84LEE73 pKa = 4.76NGLSALTTSVNCVGDD88 pKa = 4.42DD89 pKa = 3.78GDD91 pKa = 4.34SALHH95 pKa = 5.87LASLFGHH102 pKa = 6.06LQCVEE107 pKa = 4.31LLLSHH112 pKa = 6.62GAEE115 pKa = 5.08ASFTDD120 pKa = 4.31CNGGLALHH128 pKa = 6.91DD129 pKa = 4.2ACASGHH135 pKa = 6.13LDD137 pKa = 3.1IVKK140 pKa = 10.15VLLKK144 pKa = 10.39SAPHH148 pKa = 6.21TIGVIDD154 pKa = 4.54EE155 pKa = 5.54DD156 pKa = 4.73GDD158 pKa = 4.49SPLHH162 pKa = 5.26NAARR166 pKa = 11.84GNHH169 pKa = 6.0LDD171 pKa = 3.27IVSFLLDD178 pKa = 3.34NGARR182 pKa = 11.84SCLEE186 pKa = 3.52IANLEE191 pKa = 4.22GEE193 pKa = 4.6LPAMCTTCPKK203 pKa = 10.0VRR205 pKa = 11.84SLLTHH210 pKa = 5.38QTPAPP215 pKa = 3.73

Molecular weight: 22.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G4IMF8|A0A0G4IMF8_PLABS Uncharacterized protein OS=Plasmodiophora brassicae OX=37360 GN=PBRA_005102 PE=4 SV=1
MM1 pKa = 7.41RR2 pKa = 11.84LAWVVVLLATAVLTGPLQALIDD24 pKa = 4.23GVTPQQVVDD33 pKa = 4.56LLNSIRR39 pKa = 11.84GNVVPTAAQMVRR51 pKa = 11.84LTWNDD56 pKa = 3.1TLAAAAARR64 pKa = 11.84YY65 pKa = 8.01AATQCAANFDD75 pKa = 4.29GQGPQCPDD83 pKa = 3.03CANWIGEE90 pKa = 4.01GMYY93 pKa = 10.47PSATTHH99 pKa = 6.14GMLDD103 pKa = 3.83SINDD107 pKa = 3.59LMGEE111 pKa = 4.14KK112 pKa = 10.18AQWSCTQSGTTLSCKK127 pKa = 9.99CSSYY131 pKa = 10.8HH132 pKa = 7.44CYY134 pKa = 10.94DD135 pKa = 2.88NWKK138 pKa = 10.68VILNDD143 pKa = 3.29GATTVGCGEE152 pKa = 4.83LLASTCGGSYY162 pKa = 10.6NIYY165 pKa = 9.19VCAFNGFSSYY175 pKa = 10.65AHH177 pKa = 7.71PYY179 pKa = 8.47TLSATNSSCTQGTCPSAYY197 pKa = 8.76PSCVNSLCTRR207 pKa = 11.84RR208 pKa = 11.84PATQFTTRR216 pKa = 11.84RR217 pKa = 11.84TSVTKK222 pKa = 10.03MRR224 pKa = 11.84TKK226 pKa = 9.76RR227 pKa = 11.84TSVKK231 pKa = 10.18QLTKK235 pKa = 10.38RR236 pKa = 11.84RR237 pKa = 11.84TSVKK241 pKa = 10.01QLTKK245 pKa = 10.38RR246 pKa = 11.84RR247 pKa = 11.84TSVKK251 pKa = 10.01QLTKK255 pKa = 10.45RR256 pKa = 11.84RR257 pKa = 11.84TSVTTLRR264 pKa = 11.84ATLQRR269 pKa = 11.84ANVPSLRR276 pKa = 11.84KK277 pKa = 8.6RR278 pKa = 11.84TSRR281 pKa = 11.84PATGHH286 pKa = 4.27VKK288 pKa = 10.29RR289 pKa = 11.84RR290 pKa = 11.84TSTMRR295 pKa = 11.84PRR297 pKa = 11.84SRR299 pKa = 11.84LTTTPRR305 pKa = 11.84LLANSSGVSTRR316 pKa = 11.84SLEE319 pKa = 4.02PVRR322 pKa = 11.84QGSVRR327 pKa = 11.84SFVTSLGTGMAVTTPRR343 pKa = 11.84SKK345 pKa = 10.58RR346 pKa = 11.84SSSKK350 pKa = 11.01GPGADD355 pKa = 3.22TRR357 pKa = 11.84SASAPRR363 pKa = 11.84SPSLFSDD370 pKa = 3.76VSDD373 pKa = 3.27TTLFVVGSVAVVCMAALALLIRR395 pKa = 11.84RR396 pKa = 11.84RR397 pKa = 11.84NQQEE401 pKa = 3.54LSARR405 pKa = 11.84AIPEE409 pKa = 4.49FINWPTDD416 pKa = 3.48PLATSSVQSVGQSAFTGDD434 pKa = 3.94TRR436 pKa = 11.84PADD439 pKa = 4.19PPAHH443 pKa = 7.0DD444 pKa = 5.33PFNAMPKK451 pKa = 9.88AVRR454 pKa = 11.84RR455 pKa = 11.84LQARR459 pKa = 11.84GVARR463 pKa = 11.84DD464 pKa = 3.86KK465 pKa = 11.11FNQGARR471 pKa = 11.84TVSDD475 pKa = 3.82PSEE478 pKa = 4.51SDD480 pKa = 2.94AA481 pKa = 5.34

Molecular weight: 51.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9720

0

9720

4369470

23

8637

449.5

49.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.297 ± 0.031

1.859 ± 0.015

6.347 ± 0.023

4.919 ± 0.024

3.66 ± 0.017

6.112 ± 0.021

2.517 ± 0.011

4.573 ± 0.014

3.564 ± 0.02

9.544 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.375 ± 0.011

3.024 ± 0.011

5.319 ± 0.024

3.885 ± 0.015

7.378 ± 0.028

7.755 ± 0.029

5.458 ± 0.02

7.912 ± 0.019

1.245 ± 0.009

2.249 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski