Burdock mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Hepelivirales; Benyviridae; Benyvirus

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S6B9F8|S6B9F8_9VIRU Coat protein read-through product OS=Burdock mottle virus OX=1324959 GN=CP-RT PE=4 SV=1
MM1 pKa = 7.33TEE3 pKa = 3.65WASEE7 pKa = 4.02NPHH10 pKa = 6.92DD11 pKa = 4.19FFSVLEE17 pKa = 4.28RR18 pKa = 11.84NCGNAGFSWTGVRR31 pKa = 11.84PRR33 pKa = 11.84TITYY37 pKa = 10.19SDD39 pKa = 3.84LMSSGALVNLQSLLEE54 pKa = 4.22SEE56 pKa = 4.74SFSGCVRR63 pKa = 11.84SGDD66 pKa = 3.39IAAVKK71 pKa = 10.37SDD73 pKa = 3.89VVSKK77 pKa = 10.6MDD79 pKa = 3.56GKK81 pKa = 10.4DD82 pKa = 2.86WQARR86 pKa = 11.84CGLVTGVAGSGKK98 pKa = 8.53STLIKK103 pKa = 9.88TLLTSGEE110 pKa = 4.02GRR112 pKa = 11.84VVLGLPNSSLLKK124 pKa = 10.61GVFSGCPNAFLIDD137 pKa = 3.92DD138 pKa = 5.1LFTSEE143 pKa = 4.33IHH145 pKa = 5.21LQRR148 pKa = 11.84YY149 pKa = 5.38QTMLVDD155 pKa = 4.41EE156 pKa = 4.65FTKK159 pKa = 10.73VHH161 pKa = 5.5MCEE164 pKa = 4.24VMCLCVLLGVKK175 pKa = 10.29NLVCFGDD182 pKa = 4.8FSQSLNYY189 pKa = 9.86KK190 pKa = 9.56AGSVVNYY197 pKa = 8.82GLPVLAKK204 pKa = 9.92SDD206 pKa = 3.3TSKK209 pKa = 11.15RR210 pKa = 11.84FGKK213 pKa = 10.31KK214 pKa = 8.89IAGLMSGSGCGNVRR228 pKa = 11.84GSDD231 pKa = 3.6SVNDD235 pKa = 3.82DD236 pKa = 3.37VSFEE240 pKa = 4.22DD241 pKa = 5.07LMGKK245 pKa = 9.32LRR247 pKa = 11.84DD248 pKa = 3.61MSTVLVASEE257 pKa = 4.28EE258 pKa = 4.46SQKK261 pKa = 10.94EE262 pKa = 3.91LADD265 pKa = 3.87CDD267 pKa = 3.32IDD269 pKa = 4.03SFLWSEE275 pKa = 4.35VQGQTFDD282 pKa = 3.38VVEE285 pKa = 4.11VVLYY289 pKa = 10.72DD290 pKa = 3.73EE291 pKa = 5.58YY292 pKa = 11.42DD293 pKa = 3.62DD294 pKa = 6.51KK295 pKa = 11.62LICDD299 pKa = 3.74SNIRR303 pKa = 11.84TVLLSRR309 pKa = 11.84ARR311 pKa = 11.84KK312 pKa = 9.75CNVLRR317 pKa = 11.84FGPNIRR323 pKa = 11.84ARR325 pKa = 11.84FEE327 pKa = 4.22SGNFGCGGNDD337 pKa = 2.91SSYY340 pKa = 11.57SGDD343 pKa = 3.38TLRR346 pKa = 11.84EE347 pKa = 3.86EE348 pKa = 4.42RR349 pKa = 4.46

Molecular weight: 37.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S6C3R5|S6C3R5_9VIRU Capsid protein OS=Burdock mottle virus OX=1324959 GN=CP PE=4 SV=1
MM1 pKa = 6.86TRR3 pKa = 11.84EE4 pKa = 3.39IRR6 pKa = 11.84ARR8 pKa = 11.84EE9 pKa = 4.17SNTKK13 pKa = 10.34YY14 pKa = 10.17IVLGVCVVAFICFLGFSQQKK34 pKa = 9.64HH35 pKa = 4.69ATHH38 pKa = 6.54SGDD41 pKa = 3.28GVGVPRR47 pKa = 11.84FANGGSYY54 pKa = 10.5RR55 pKa = 11.84DD56 pKa = 3.89GTRR59 pKa = 11.84SMNFNSNNPNAYY71 pKa = 8.42GCKK74 pKa = 9.7SEE76 pKa = 4.62GFFGGFEE83 pKa = 3.98KK84 pKa = 10.85LALLFLVLGIILYY97 pKa = 9.96VAGGCAGGDD106 pKa = 3.58HH107 pKa = 6.4VCNGGCCKK115 pKa = 10.3VV116 pKa = 3.14

Molecular weight: 12.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

3711

116

2217

530.1

58.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.114 ± 0.945

1.967 ± 0.614

6.117 ± 0.379

5.901 ± 0.482

5.012 ± 0.228

6.871 ± 0.949

2.129 ± 0.213

4.554 ± 0.436

5.74 ± 0.415

9.054 ± 0.396

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.506 ± 0.263

3.961 ± 0.248

3.719 ± 0.407

3.288 ± 0.42

5.039 ± 0.186

8.946 ± 0.581

5.147 ± 0.238

8.569 ± 1.053

1.293 ± 0.211

3.045 ± 0.405
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski